FastQCFastQC Report
Fri 10 Feb 2017
ERR1633146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749437
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34040.4542076251906431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23690.31610395536916375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14300.19080990129924197No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT12790.1706614431900213No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9740.12996422647934383No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9560.12756242352592678No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8660.11555340875884162No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8500.11341847280024872No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8420.11235100482095225No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7760.10354439399175648No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT7570.10100915754092739No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGC250.005495138529.622
GGTATCA7100.022.9295771
GTATCAA18550.022.4393521
TACGTCA450.003824584720.5555555
GCTATAC609.232727E-418.53
GGCCGTT1156.3961124E-817.69565214
GCCGTTA1054.791764E-717.61904715
CGTCGGT752.0660566E-417.26666619
CCCCTAG650.001579240217.0769231
CGAGACT650.001579240217.0769234
CCGTTAA1107.7986806E-716.81818216
ATAGAAC1005.8723017E-616.6500023
CAACCCA1801.0913936E-1116.44444517
GTACTAT904.442636E-516.4444456
GTATTAG2950.016.3050841
CGATCGG700.002591364615.8571435
ATCAACG26200.015.6049623
CTTATGC957.054436E-515.5789483
TCAACCC1902.7284841E-1115.57894816
AACGCAG26900.015.4739776
Produced by FastQC (version 0.11.5)