##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633146.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	749437
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85817220126575	33.0	33.0	33.0	33.0	33.0
2	31.97772728061198	33.0	33.0	33.0	33.0	33.0
3	31.69950776382805	33.0	33.0	33.0	27.0	33.0
4	31.975358836032914	33.0	33.0	33.0	33.0	33.0
5	32.07093991889912	33.0	33.0	33.0	33.0	33.0
6	35.23850303627923	37.0	37.0	37.0	33.0	37.0
7	35.37682820570642	37.0	37.0	37.0	33.0	37.0
8	35.50216095549059	37.0	37.0	37.0	33.0	37.0
9	35.62046843163601	37.0	37.0	37.0	33.0	37.0
10	35.63706222137418	37.0	37.0	37.0	33.0	37.0
11	35.65405230859965	37.0	37.0	37.0	33.0	37.0
12	35.641792438857436	37.0	37.0	37.0	33.0	37.0
13	35.63657385477365	37.0	37.0	37.0	33.0	37.0
14	35.617117916516	37.0	37.0	37.0	33.0	37.0
15	35.64039805880948	37.0	37.0	37.0	33.0	37.0
16	35.62157726399951	37.0	37.0	37.0	33.0	37.0
17	35.61424375898174	37.0	37.0	37.0	33.0	37.0
18	35.596750627471025	37.0	37.0	37.0	33.0	37.0
19	35.59202841599761	37.0	37.0	37.0	33.0	37.0
20	35.600824352147015	37.0	37.0	37.0	33.0	37.0
21	35.594178029640915	37.0	37.0	37.0	33.0	37.0
22	35.50046368140351	37.0	37.0	37.0	33.0	37.0
23	35.54897743239258	37.0	37.0	37.0	33.0	37.0
24	35.537779693289764	37.0	37.0	37.0	33.0	37.0
25	35.55907968248165	37.0	37.0	37.0	33.0	37.0
26	35.45145088913411	37.0	37.0	37.0	33.0	37.0
27	35.472095719853705	37.0	37.0	37.0	33.0	37.0
28	35.48991442909811	37.0	37.0	37.0	33.0	37.0
29	35.50664832400856	37.0	37.0	37.0	33.0	37.0
30	35.50455742110411	37.0	37.0	37.0	33.0	37.0
31	35.48968625781753	37.0	37.0	37.0	33.0	37.0
32	35.487543315849095	37.0	37.0	37.0	33.0	37.0
33	35.48022849152097	37.0	37.0	37.0	33.0	37.0
34	35.461517112178875	37.0	37.0	37.0	33.0	37.0
35	35.40767002429824	37.0	37.0	37.0	33.0	37.0
36	35.438236969885395	37.0	37.0	37.0	33.0	37.0
37	35.440171755597866	37.0	37.0	37.0	33.0	37.0
38	35.422634591033	37.0	37.0	37.0	33.0	37.0
39	35.32105300378818	37.0	37.0	37.0	33.0	37.0
40	35.13684539194088	37.0	37.0	37.0	33.0	37.0
41	35.293445613173624	37.0	37.0	37.0	33.0	37.0
42	35.333849276189994	37.0	37.0	37.0	33.0	37.0
43	34.99684563212118	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	17.0
16	38.0
17	42.0
18	19.0
19	28.0
20	44.0
21	132.0
22	341.0
23	844.0
24	1675.0
25	2992.0
26	4881.0
27	7446.0
28	10432.0
29	13896.0
30	18162.0
31	23041.0
32	29337.0
33	38897.0
34	56593.0
35	115422.0
36	425153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24789675449704	20.697670384568685	14.202127730549732	23.85230513038454
2	15.80773300490902	21.671201181687053	39.64856285451612	22.872502958887804
3	17.80363125919857	27.24631957055763	30.64353641466861	24.306512755575184
4	12.658168731994818	17.91825063347553	39.16099685497247	30.26258377955719
5	13.596606519293816	37.62210832931921	35.37188582896228	13.4093993224247
6	29.314538780444522	39.58024490384115	17.478854126497627	13.626362189216707
7	25.913185497913766	32.896294151476376	23.475222066698066	17.71529828391179
8	24.096354997151195	36.6016089411118	20.59626092653552	18.705775135201492
9	25.525427754434325	16.232318393674188	20.136182227458747	38.106071624432744
10	14.867827449138488	28.68499953965443	33.71090565317699	22.736267358030094
11	33.11152238280202	24.28609743047114	24.287031464953024	18.315348721773812
12	22.55306316608334	26.24450087198791	30.039883272376468	21.162552689552292
13	27.308499580351654	22.498488865641807	27.05310786630497	23.13990368770157
14	21.7171023047968	21.584069107876978	27.69559015634403	29.003238430982194
15	23.724876140356027	28.934787046809806	25.071486996238512	22.268849816595658
16	22.815526855492855	29.100378017098166	24.999699774630823	23.084395352778152
17	22.212674314185183	28.00835827427789	26.425436694478655	23.35353071705827
18	22.27165192004131	27.75216595924674	27.981938441790305	21.99424367892164
19	24.460628445086112	26.805455295108192	28.058796136299648	20.675120123506044
20	24.064330957772302	26.29026856160024	28.384107002990245	21.261293477637214
21	22.247100156517494	27.84103266852317	27.571497003750817	22.34037017120852
22	22.149293402914456	27.506381457013735	27.5768343436473	22.767490796424518
23	22.077906481799005	27.478226989059788	28.13085022490216	22.31301630423905
24	22.990324737102654	27.858779323678974	26.916471964955026	22.234423974263347
25	23.038894530160643	26.90299518171641	27.901878343343068	22.15623194477988
26	22.5648053138556	27.21429553117874	27.757236432148396	22.46366272281726
27	22.738135426993864	26.903662349203465	27.381487703436047	22.976714520366624
28	21.85507254112087	27.409909038384818	28.249205737106653	22.485812683387664
29	23.282277229440233	27.420717151675188	27.293154728149265	22.003850890735315
30	22.52637646660093	27.447003550665368	27.730549732665988	22.296070250067718
31	22.813525353031675	27.079394265295146	27.784189998625635	22.322890383047543
32	21.804901546093937	27.179736255349013	27.976734535391234	23.038627663165816
33	22.1011239103487	26.981987812184343	28.29163758928369	22.625250688183264
34	22.240161614652067	26.985323649619648	27.98367307725666	22.790841658471624
35	22.63872747142188	27.585907821471316	27.511985663905037	22.263379043201763
36	22.941888377542075	27.498242013671597	27.19054436863939	22.369325240146935
37	23.100273938970187	26.534051561372067	27.356935939912226	23.008738559745513
38	23.03088852031592	27.258862319314364	27.431925565457803	22.278323594911914
39	22.550661363129922	27.172797713483586	27.81327850106146	22.463262422325027
40	22.701307781708135	27.15665226029673	27.796332446890133	22.345707511105005
41	22.298071752528898	27.135703201203036	27.933368648732316	22.63285639753575
42	22.558133638984998	27.290486058201026	27.495973644215592	22.655406658598388
43	22.56293724489183	26.729798502075557	27.694656121862142	23.012608131170463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	46.5
2	59.0
3	196.0
4	333.0
5	333.0
6	424.5
7	516.0
8	458.0
9	400.0
10	608.0
11	816.0
12	816.0
13	1247.5
14	1679.0
15	3394.0
16	5109.0
17	5800.5
18	6492.0
19	6492.0
20	6259.0
21	6026.0
22	6912.5
23	7799.0
24	9754.5
25	11710.0
26	11710.0
27	14175.5
28	16641.0
29	21227.0
30	25813.0
31	28403.0
32	30993.0
33	30993.0
34	35555.5
35	40118.0
36	42550.5
37	44983.0
38	49479.5
39	53976.0
40	53976.0
41	57330.0
42	60684.0
43	58151.5
44	55619.0
45	56428.0
46	57237.0
47	57237.0
48	56153.0
49	55069.0
50	55978.5
51	56888.0
52	55739.0
53	54590.0
54	54590.0
55	48525.5
56	42461.0
57	37099.0
58	31737.0
59	28470.5
60	25204.0
61	25204.0
62	22265.5
63	19327.0
64	15944.5
65	12562.0
66	10661.0
67	8760.0
68	8760.0
69	7259.0
70	5758.0
71	4905.5
72	4053.0
73	3327.0
74	2601.0
75	2601.0
76	1999.0
77	1397.0
78	1100.0
79	803.0
80	630.5
81	458.0
82	458.0
83	383.5
84	309.0
85	272.0
86	235.0
87	180.5
88	126.0
89	126.0
90	88.0
91	50.0
92	30.0
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	749437.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.52648355360963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.85318896286917	46.812724960088254
2	8.060233561515394	8.78992385460443
3	2.1697064095659018	3.5491938257206965
4	0.9870444673957041	2.1528025567253297
5	0.561606368318858	1.5311210202870322
6	0.34865799735970426	1.140665673532103
7	0.2743507245470082	1.047156618895333
8	0.19101774685128511	0.8332420825707333
9	0.15121704602908853	0.7420800395997473
>10	1.1119285339317557	12.344091703157755
>50	0.16578073400421495	6.2952164525733725
>100	0.11905378453589026	11.647718116011404
>500	0.005219476983801651	1.9645512508924075
>1k	9.941860921526954E-4	1.1495118453414321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3404	0.4542076251906431	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2369	0.31610395536916375	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1430	0.19080990129924197	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1279	0.1706614431900213	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	974	0.12996422647934383	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	956	0.12756242352592678	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	866	0.11555340875884162	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	850	0.11341847280024872	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	842	0.11235100482095225	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	776	0.10354439399175648	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	757	0.10100915754092739	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.334334974120573E-4	0.0	0.0
2	0.0	0.0	1.334334974120573E-4	0.0	0.0
3	0.0	0.0	5.337339896482292E-4	0.0	0.0
4	0.0	0.0	6.671674870602866E-4	0.0	0.0
5	1.334334974120573E-4	0.0	6.671674870602866E-4	0.0	0.0
6	1.334334974120573E-4	0.0	8.006009844723439E-4	0.0	0.0
7	1.334334974120573E-4	0.0	0.001734635466356745	0.0	0.0
8	1.334334974120573E-4	0.0	0.002535236450829089	0.0	0.0
9	1.334334974120573E-4	0.0	0.002935536943065261	0.0010674679792964585	0.0
10	1.334334974120573E-4	0.0	0.0032024039378893755	0.0010674679792964585	0.0
11	1.334334974120573E-4	0.0	0.004937039404246121	0.0013343349741205732	0.0
12	1.334334974120573E-4	0.0	0.005070472901658178	0.0014677684715326306	0.0
13	1.334334974120573E-4	0.0	0.0052039063990702355	0.0014677684715326306	0.0
14	1.334334974120573E-4	0.0	0.00547077339389435	0.0016012019689446877	0.0
15	1.334334974120573E-4	0.0	0.006271374378366694	0.001734635466356745	0.0
16	1.334334974120573E-4	0.0	0.00693854186542698	0.001734635466356745	0.0
17	1.334334974120573E-4	0.0	0.0073388423576631525	0.0022683694560049743	0.0
18	1.334334974120573E-4	0.0	0.00747227585507521	0.0022683694560049743	0.0
19	1.334334974120573E-4	0.0	0.007739142849899325	0.0024018029534170317	0.0
20	1.334334974120573E-4	0.0	0.007739142849899325	0.0024018029534170317	0.0
21	1.334334974120573E-4	0.0	0.008139443342135497	0.0024018029534170317	0.0
22	1.334334974120573E-4	0.0	0.008139443342135497	0.003068970440477318	0.0
23	1.334334974120573E-4	0.0	0.008139443342135497	0.003335837435301433	0.0
24	1.334334974120573E-4	0.0	0.008139443342135497	0.00346927093271349	0.0
25	1.334334974120573E-4	0.0	0.008139443342135497	0.004136438419773777	0.0
26	1.334334974120573E-4	0.0	0.008139443342135497	0.004269871917185834	0.0
27	1.334334974120573E-4	0.0	0.008139443342135497	0.004403305414597891	0.0
28	1.334334974120573E-4	0.0	0.008139443342135497	0.010674679792964586	0.0
29	1.334334974120573E-4	0.0	0.008139443342135497	0.022016527072989456	0.0
30	1.334334974120573E-4	0.0	0.008139443342135497	0.03776167976761222	0.0
31	1.334334974120573E-4	0.0	0.008139443342135497	0.08179473391359114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTCGC	25	0.0054951385	29.6	22
GGTATCA	710	0.0	22.929577	1
GTATCAA	1855	0.0	22.439352	1
TACGTCA	45	0.0038245847	20.555555	5
GCTATAC	60	9.232727E-4	18.5	3
GGCCGTT	115	6.3961124E-8	17.695652	14
GCCGTTA	105	4.791764E-7	17.619047	15
CGTCGGT	75	2.0660566E-4	17.266666	19
CCCCTAG	65	0.0015792402	17.076923	1
CGAGACT	65	0.0015792402	17.076923	4
CCGTTAA	110	7.7986806E-7	16.818182	16
ATAGAAC	100	5.8723017E-6	16.650002	3
CAACCCA	180	1.0913936E-11	16.444445	17
GTACTAT	90	4.442636E-5	16.444445	6
GTATTAG	295	0.0	16.305084	1
CGATCGG	70	0.0025913646	15.857143	5
ATCAACG	2620	0.0	15.604962	3
CTTATGC	95	7.054436E-5	15.578948	3
TCAACCC	190	2.7284841E-11	15.578948	16
AACGCAG	2690	0.0	15.473977	6
>>END_MODULE