FastQCFastQC Report
Fri 10 Feb 2017
ERR1633145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756503
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48900.6463953216312427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34530.4564423406120002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19920.2633168672166535No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9340.12346282830339073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.11883627692157203No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8580.11341660244572724No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8490.11222691780468814No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7930.10482443559377821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.027.8641971
CGGCGTC358.8665815E-426.4285724
CGCTATC509.085725E-625.90000212
GTATCAA23750.023.9136851
CGAATTA400.001930580223.12515
TTACCGG502.7009193E-422.231
TTTGCCG806.953651E-720.81259
TTTACCG656.898145E-519.92307730
TGCGGGT1303.274181E-1119.92307721
CCCGAAT656.898145E-519.92307713
GCCGAGT851.244156E-619.58823612
GGACCGT1052.2562745E-819.380956
CGGGTAA1153.0449883E-919.30434823
CGAGTTC902.1502383E-618.514
CCGAATT500.007032713418.514
TAGGACC1850.018.04
GCGGGTA1258.563802E-917.7622
TTGCCGA1054.7919093E-717.61904710
GGCTATA1054.7919093E-717.61904733
CGAGGGT1800.017.47222116
Produced by FastQC (version 0.11.5)