FastQCFastQC Report
Fri 10 Feb 2017
ERR1633144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648293
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36120.5571554837087551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.37498476768991795No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG17710.27317894840758733No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA16700.257599573032564No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG15140.2335363793840131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14560.22458980738647494No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG13370.20623390966738805No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG13070.20160637242728213No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC10640.164123320782424No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT10140.15641075871558077No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA9920.15301723140616974No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT9310.143607905684621No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC9000.1388261172031782No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC8750.13496983616975658No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG8590.13250181630836674No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8570.13219331382569302No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT8520.1314220576190087No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG8510.13126780637767183No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7800.12031596824275442No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7650.11800219962270146No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7220.11136939624521629No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT7070.10905562762516333No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG7000.10797586893580526No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC6710.10350258293703618No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA6640.10242282424767814No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA6520.10057180935163575No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6520.10057180935163575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA405.9361977E-527.7532
GGTATCA6550.025.7022911
TCGCAAG400.001930284423.12533
ACGTATA400.001930284423.12529
ACAACGC502.7003407E-422.23
GTATCAA16600.022.0662651
GACGTAT450.003824034520.55555728
GCCGGCA1001.2871169E-820.3515
TGCGGTA555.1397685E-420.18181836
GGGCGAC555.1397685E-420.18181831
GTTCTAC656.896198E-519.9230771
ACCGCAT701.2181568E-418.522
CGCTCTC902.1492706E-618.529
CATAGAT500.00703165318.53
CGCAAGA500.00703165318.534
ATACCGC500.00703165318.520
CCTTGCG701.2181568E-418.533
TCTATGG609.230893E-418.53
TATACAG1054.78929E-717.6190475
CCGCTCT953.6014808E-617.52631628
Produced by FastQC (version 0.11.5)