##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633144.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648293
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.695862827456104	33.0	33.0	33.0	27.0	33.0
2	31.837184729744113	33.0	33.0	33.0	33.0	33.0
3	31.565687119867096	33.0	33.0	33.0	27.0	33.0
4	31.87004332917369	33.0	33.0	33.0	27.0	33.0
5	31.97627615908239	33.0	33.0	33.0	33.0	33.0
6	35.0958023609695	37.0	37.0	37.0	33.0	37.0
7	35.24442805953481	37.0	37.0	37.0	33.0	37.0
8	35.380753764115916	37.0	37.0	37.0	33.0	37.0
9	35.50927435588538	37.0	37.0	37.0	33.0	37.0
10	35.51931302667158	37.0	37.0	37.0	33.0	37.0
11	35.514338424138465	37.0	37.0	37.0	33.0	37.0
12	35.48786891112506	37.0	37.0	37.0	33.0	37.0
13	35.508375071148386	37.0	37.0	37.0	33.0	37.0
14	35.507454191237606	37.0	37.0	37.0	33.0	37.0
15	35.52788014061543	37.0	37.0	37.0	33.0	37.0
16	35.50418097989643	37.0	37.0	37.0	33.0	37.0
17	35.489605780102515	37.0	37.0	37.0	33.0	37.0
18	35.47677670436053	37.0	37.0	37.0	33.0	37.0
19	35.469531523554934	37.0	37.0	37.0	33.0	37.0
20	35.455542478478094	37.0	37.0	37.0	33.0	37.0
21	35.46117110627448	37.0	37.0	37.0	33.0	37.0
22	35.37891971685642	37.0	37.0	37.0	33.0	37.0
23	35.418512925482766	37.0	37.0	37.0	33.0	37.0
24	35.40540619750637	37.0	37.0	37.0	33.0	37.0
25	35.42840197256488	37.0	37.0	37.0	33.0	37.0
26	35.31740432181128	37.0	37.0	37.0	33.0	37.0
27	35.3177992049891	37.0	37.0	37.0	33.0	37.0
28	35.351186886176464	37.0	37.0	37.0	33.0	37.0
29	35.34886694750676	37.0	37.0	37.0	33.0	37.0
30	35.35859403078546	37.0	37.0	37.0	33.0	37.0
31	35.35381686984126	37.0	37.0	37.0	33.0	37.0
32	35.3420752653507	37.0	37.0	37.0	33.0	37.0
33	35.34364862801233	37.0	37.0	37.0	33.0	37.0
34	35.311137093875764	37.0	37.0	37.0	33.0	37.0
35	35.23655507617698	37.0	37.0	37.0	33.0	37.0
36	35.27989813248022	37.0	37.0	37.0	33.0	37.0
37	35.26972680562647	37.0	37.0	37.0	33.0	37.0
38	35.26959723458374	37.0	37.0	37.0	33.0	37.0
39	35.16650495994867	37.0	37.0	37.0	33.0	37.0
40	34.95077534386458	37.0	37.0	37.0	27.0	37.0
41	35.11824900160884	37.0	37.0	37.0	33.0	37.0
42	35.16487143930291	37.0	37.0	37.0	33.0	37.0
43	34.808557241864406	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	31.0
17	23.0
18	23.0
19	22.0
20	37.0
21	154.0
22	358.0
23	818.0
24	1696.0
25	3042.0
26	4879.0
27	7079.0
28	10060.0
29	13232.0
30	17427.0
31	22005.0
32	27743.0
33	36546.0
34	52493.0
35	104120.0
36	346489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32747692787058	20.24424141553279	13.164726443136052	23.26355521346058
2	16.68844180023539	22.05885301861967	38.65366431536358	22.599040865781365
3	18.20689101995548	26.871028994605833	29.588010359513365	25.334069625925316
4	12.239218995114863	17.959163526368478	39.55109803746146	30.2505194410552
5	14.342588921984351	36.272796405329075	35.0071649701601	14.377449702526482
6	29.887874772672234	39.794352245049694	17.107850925430323	13.209922056847754
7	28.238157746574466	31.606387852406243	22.64454498197574	17.51090941904355
8	24.92977712238139	35.295152037736024	20.287740265589786	19.4873305742928
9	26.115814917020547	15.201768336230687	20.32938810075074	38.35302864599803
10	15.468622983743463	27.959271502237414	33.4493816838991	23.122723830120023
11	34.3606980177173	24.192456188791176	23.166993936383705	18.279851857107822
12	23.43677935748188	25.485235842435443	30.09811921461438	20.9798655854683
13	28.158255603561972	22.481964173606688	26.192323532723627	23.167456690107713
14	22.59780685585067	21.11576092908608	27.526442519046174	28.75998969601708
15	23.731244977193956	28.748883606640824	25.393302102598668	22.126569313566552
16	23.930229078518508	28.456577504307468	24.571297237514518	23.041896179659503
17	23.320011167789872	26.969749789061424	25.67265110065973	24.037587942488965
18	22.97880742195273	26.335005930960232	28.493752053469652	22.192434593617392
19	26.08450191502916	25.99858397360453	27.164260604387213	20.752653506979097
20	25.108400059849483	26.129697528740863	27.45903472658196	21.302867684827696
21	22.938702099205145	27.224264337267257	27.1897120592078	22.647321504319805
22	23.87315611922387	25.93148468362299	27.47399709699164	22.721362100161503
23	22.39512072473403	26.625461018397544	28.453029725756718	22.526388531111703
24	22.99392404360374	27.6658856412147	26.472289535750036	22.867900779431523
25	23.01181718759882	27.340878275717923	27.86980578226203	21.777498754421227
26	24.496793887948815	26.53460703725013	26.75472355863784	22.213875516163217
27	23.56018035055137	26.545867377867722	26.281635001457676	23.61231727012323
28	22.94317538520391	26.313256505931733	28.112288733643588	22.63127937522077
29	24.501575676430257	26.960957468305224	27.088523244890812	21.448943610373703
30	23.853566211574087	26.316033028275793	27.00846685063698	22.821933909513138
31	22.984977471606204	25.84494973723301	27.69704439196474	23.473028399196043
32	22.258299873668232	26.544633367937028	27.55605875738285	23.641008001011887
33	22.79663670593389	25.876262739224394	28.3416603295115	22.985440225330215
34	23.756233678290524	26.03298200042265	27.924873475419293	22.285910845867534
35	23.520229279045125	27.116442719572788	26.436194745277213	22.927133256104877
36	23.44325790961803	26.373105987570433	26.611732657918562	23.57190344489297
37	23.51992077656245	26.097150516818786	27.416615635214324	22.966313071404443
38	23.18180205555204	25.818418523723068	26.96543075430399	24.034348666420893
39	22.903687067421675	26.34919704516322	27.526288267804834	23.22082761961027
40	23.580850016890512	27.142973933082725	27.080347929099958	22.1958281209268
41	23.17300973479584	26.097150516818786	27.428338729555925	23.30150101882945
42	22.220508319540702	28.221498612510082	26.82506212468745	22.732930943261767
43	24.43833266748214	25.470581974508438	26.511469351049605	23.579616006959817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	44.5
2	66.0
3	203.0
4	340.0
5	340.0
6	442.5
7	545.0
8	511.0
9	477.0
10	615.5
11	754.0
12	754.0
13	1238.5
14	1723.0
15	3087.0
16	4451.0
17	5216.0
18	5981.0
19	5981.0
20	5499.0
21	5017.0
22	5549.5
23	6082.0
24	7581.0
25	9080.0
26	9080.0
27	10829.5
28	12579.0
29	14646.0
30	16713.0
31	19443.5
32	22174.0
33	22174.0
34	29278.5
35	36383.0
36	39074.5
37	41766.0
38	42686.5
39	43607.0
40	43607.0
41	44123.0
42	44639.0
43	46217.5
44	47796.0
45	47014.5
46	46233.0
47	46233.0
48	43794.0
49	41355.0
50	43514.5
51	45674.0
52	46789.5
53	47905.0
54	47905.0
55	46983.5
56	46062.0
57	38312.0
58	30562.0
59	28537.5
60	26513.0
61	26513.0
62	24424.0
63	22335.0
64	17077.0
65	11819.0
66	10015.0
67	8211.0
68	8211.0
69	7167.0
70	6123.0
71	6588.5
72	7054.0
73	6425.0
74	5796.0
75	5796.0
76	3422.0
77	1048.0
78	793.0
79	538.0
80	430.5
81	323.0
82	323.0
83	280.5
84	238.0
85	195.0
86	152.0
87	123.0
88	94.0
89	94.0
90	71.0
91	48.0
92	28.0
93	8.0
94	7.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	648293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.28428218625889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98110823781128	44.60763699741441
2	7.362430120213682	7.551538877233009
3	1.8702411425412464	2.8774192353130954
4	0.8570011094815949	1.7580274693036426
5	0.5276164272723449	1.3529214871170339
6	0.3402048777818103	1.0468297771982447
7	0.25929825593731626	0.9308547449525874
8	0.19776336585147875	0.8113721808345272
9	0.15918925379986262	0.7347515951602926
>10	1.1251138423840608	11.65646830089238
>50	0.16461486573015752	5.925229588040142
>100	0.14223705040342238	14.506829444279216
>500	0.010115997119208901	3.5398883643468557
>1k	0.0030654536724875462	2.700231937914535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3612	0.5571554837087551	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2431	0.37498476768991795	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1771	0.27317894840758733	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1670	0.257599573032564	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1514	0.2335363793840131	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1456	0.22458980738647494	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1337	0.20623390966738805	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1307	0.20160637242728213	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1064	0.164123320782424	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	1014	0.15641075871558077	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	992	0.15301723140616974	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	931	0.143607905684621	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	900	0.1388261172031782	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	875	0.13496983616975658	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	859	0.13250181630836674	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	857	0.13219331382569302	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	852	0.1314220576190087	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	851	0.13126780637767183	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	780	0.12031596824275442	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	765	0.11800219962270146	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	722	0.11136939624521629	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	707	0.10905562762516333	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	700	0.10797586893580526	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	671	0.10350258293703618	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	664	0.10242282424767814	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	652	0.10057180935163575	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	652	0.10057180935163575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.5425124133686466E-4	0.0	0.0
2	1.5425124133686466E-4	0.0	1.5425124133686466E-4	0.0	0.0
3	1.5425124133686466E-4	0.0	3.085024826737293E-4	0.0	0.0
4	1.5425124133686466E-4	0.0	4.62753724010594E-4	0.0	0.0
5	1.5425124133686466E-4	0.0	9.25507448021188E-4	0.0	0.0
6	1.5425124133686466E-4	0.0	0.0012340099306949173	0.0	0.0
7	1.5425124133686466E-4	0.0	0.00231376862005297	0.0	0.0
8	1.5425124133686466E-4	0.0	0.0026222711027266993	0.0	0.0
9	1.5425124133686466E-4	0.0	0.0033935273094110223	0.0010797586893580525	0.0
10	7.712562066843233E-4	0.0	0.004164783516095346	0.0010797586893580525	0.0
11	7.712562066843233E-4	0.0	0.006324300894811451	0.0010797586893580525	0.0
12	7.712562066843233E-4	0.0	0.00663280337748518	0.0010797586893580525	0.0
13	7.712562066843233E-4	0.0	0.007095557101495774	0.0010797586893580525	0.0
14	7.712562066843233E-4	0.0	0.0075583108255063685	0.0010797586893580525	0.0
15	7.712562066843233E-4	0.0	0.007712562066843233	0.0013882611720317818	0.0
16	7.712562066843233E-4	0.0	0.008175315790853828	0.0013882611720317818	0.0
17	7.712562066843233E-4	0.0	0.00863806951486442	0.0013882611720317818	0.0
18	7.712562066843233E-4	0.0	0.008792320756201285	0.0016967636547055112	0.0
19	7.712562066843233E-4	0.0	0.00925507448021188	0.001851014896042376	0.0
20	7.712562066843233E-4	0.0	0.009563576962885609	0.001851014896042376	0.0
21	7.712562066843233E-4	0.0	0.009872079445559338	0.00231376862005297	0.0
22	7.712562066843233E-4	0.0	0.010180581928233068	0.0027765223440635637	0.0
23	7.712562066843233E-4	0.0	0.010334833169569933	0.0033935273094110223	0.0
24	7.712562066843233E-4	0.0	0.010334833169569933	0.00431903475743221	0.0
25	7.712562066843233E-4	0.0	0.010334833169569933	0.004781788481442804	0.0
26	7.712562066843233E-4	0.0	0.010334833169569933	0.005553044688127127	0.0
27	7.712562066843233E-4	0.0	0.010334833169569933	0.00663280337748518	0.0
28	7.712562066843233E-4	0.0	0.010334833169569933	0.014808119168339007	0.0
29	7.712562066843233E-4	0.0	0.010334833169569933	0.031004499508709798	0.0
30	7.712562066843233E-4	0.0	0.010334833169569933	0.05244542205453399	0.0
31	9.25507448021188E-4	0.0	0.010334833169569933	0.10874712514248959	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAA	40	5.9361977E-5	27.75	32
GGTATCA	655	0.0	25.702291	1
TCGCAAG	40	0.0019302844	23.125	33
ACGTATA	40	0.0019302844	23.125	29
ACAACGC	50	2.7003407E-4	22.2	3
GTATCAA	1660	0.0	22.066265	1
GACGTAT	45	0.0038240345	20.555557	28
GCCGGCA	100	1.2871169E-8	20.35	15
TGCGGTA	55	5.1397685E-4	20.181818	36
GGGCGAC	55	5.1397685E-4	20.181818	31
GTTCTAC	65	6.896198E-5	19.923077	1
ACCGCAT	70	1.2181568E-4	18.5	22
CGCTCTC	90	2.1492706E-6	18.5	29
CATAGAT	50	0.007031653	18.5	3
CGCAAGA	50	0.007031653	18.5	34
ATACCGC	50	0.007031653	18.5	20
CCTTGCG	70	1.2181568E-4	18.5	33
TCTATGG	60	9.230893E-4	18.5	3
TATACAG	105	4.78929E-7	17.619047	5
CCGCTCT	95	3.6014808E-6	17.526316	28
>>END_MODULE