FastQCFastQC Report
Fri 10 Feb 2017
ERR1633143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929978
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72000.7742118630763308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48220.5185068894102871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28570.3072115684457052No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15040.16172425584261133No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14720.15828331422893877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.13634731144177603No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11820.12709978085503099No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11700.12580942774990375No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC11540.12408895694306747No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11450.12312119211422205No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11170.12011036820225855No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10650.11451883808004061No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10560.11355107325119519No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10550.1134435438257679No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10280.11054024933923168No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9820.10559389576957734No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9750.10484118979158646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.025.069391
GTATCAA34400.023.5014531
ACGGTGT400.001930911823.12535
CCGCTCT3100.020.88709628
CTGATCG555.142081E-420.1818188
TTAACGG3000.019.73333435
ATTAGAG3500.019.5571443
AGCTTCG3400.019.04411721
GCCGGCA3500.019.02857215
GCCGCTC3550.018.76056327
TTCGCCG3550.018.76056324
GTATTAG5100.018.51
TCGGGTA500.00703389618.525
CGCTCTC3400.018.529
ACGGTAC2000.018.53
TGCTCGC3750.018.25333410
AACGGCC3350.018.2238837
CGCCGGC3950.017.79746814
TATTAGA3550.017.718312
TGTCCGG2850.017.52631814
Produced by FastQC (version 0.11.5)