##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	929978
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82422810001957	33.0	33.0	33.0	27.0	33.0
2	31.939152324033472	33.0	33.0	33.0	33.0	33.0
3	31.658836015475636	33.0	33.0	33.0	27.0	33.0
4	31.950944000825825	33.0	33.0	33.0	33.0	33.0
5	32.047646288406824	33.0	33.0	33.0	33.0	33.0
6	35.186903346100664	37.0	37.0	37.0	33.0	37.0
7	35.34562860626811	37.0	37.0	37.0	33.0	37.0
8	35.47467251913486	37.0	37.0	37.0	33.0	37.0
9	35.59655282167965	37.0	37.0	37.0	33.0	37.0
10	35.583364337651	37.0	37.0	37.0	33.0	37.0
11	35.60320674252509	37.0	37.0	37.0	33.0	37.0
12	35.5863224721445	37.0	37.0	37.0	33.0	37.0
13	35.586839688680804	37.0	37.0	37.0	33.0	37.0
14	35.56769299918923	37.0	37.0	37.0	33.0	37.0
15	35.596264642819506	37.0	37.0	37.0	33.0	37.0
16	35.5816030056625	37.0	37.0	37.0	33.0	37.0
17	35.570969420782	37.0	37.0	37.0	33.0	37.0
18	35.54950762276097	37.0	37.0	37.0	33.0	37.0
19	35.53762132007424	37.0	37.0	37.0	33.0	37.0
20	35.53782024951128	37.0	37.0	37.0	33.0	37.0
21	35.53174913815165	37.0	37.0	37.0	33.0	37.0
22	35.441964218508396	37.0	37.0	37.0	33.0	37.0
23	35.48887177976253	37.0	37.0	37.0	33.0	37.0
24	35.47753602773399	37.0	37.0	37.0	33.0	37.0
25	35.50134089193508	37.0	37.0	37.0	33.0	37.0
26	35.39157915563594	37.0	37.0	37.0	33.0	37.0
27	35.40816449421384	37.0	37.0	37.0	33.0	37.0
28	35.416958250625285	37.0	37.0	37.0	33.0	37.0
29	35.42037015929409	37.0	37.0	37.0	33.0	37.0
30	35.422341173662176	37.0	37.0	37.0	33.0	37.0
31	35.406285955151624	37.0	37.0	37.0	33.0	37.0
32	35.402214891104954	37.0	37.0	37.0	33.0	37.0
33	35.390239338995116	37.0	37.0	37.0	33.0	37.0
34	35.36609360651542	37.0	37.0	37.0	33.0	37.0
35	35.29790597196923	37.0	37.0	37.0	33.0	37.0
36	35.32248504803339	37.0	37.0	37.0	33.0	37.0
37	35.31615156487573	37.0	37.0	37.0	33.0	37.0
38	35.292458531277084	37.0	37.0	37.0	33.0	37.0
39	35.19697132620342	37.0	37.0	37.0	33.0	37.0
40	34.9709702810174	37.0	37.0	37.0	27.0	37.0
41	35.1397000789266	37.0	37.0	37.0	33.0	37.0
42	35.16798246840248	37.0	37.0	37.0	33.0	37.0
43	34.8181580639542	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	28.0
16	41.0
17	36.0
18	30.0
19	45.0
20	65.0
21	172.0
22	475.0
23	1141.0
24	2279.0
25	4006.0
26	6326.0
27	9594.0
28	13474.0
29	18264.0
30	23967.0
31	30375.0
32	38815.0
33	50774.0
34	72842.0
35	145678.0
36	511547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.745849901825636	19.469492826712028	13.842155405826803	23.94250186563553
2	16.428560675628887	21.484271670942753	39.37146900249253	22.715698650935828
3	18.338820918344307	27.733989406201008	29.519838103697076	24.407351571757612
4	12.38513169128732	17.92698321895787	39.772876347612524	29.915008742142284
5	14.135710737243246	36.678394542666595	34.42221213835166	14.763682581738493
6	28.78767024596281	40.08567944618045	17.054596990466443	14.072053317390305
7	26.74826716330924	32.35517399336329	22.709892061962755	18.18666678136472
8	24.87220127787969	34.66877711085638	20.13628279378652	20.322738817477404
9	26.863108589665565	14.914008718485814	19.808102987382497	38.41477970446613
10	16.148016404689145	27.293226291374634	32.48786530434054	24.070891999595688
11	34.29124129818125	24.093903296637126	23.100761523390876	18.514093881790753
12	24.05508517405788	25.87125716952444	28.966169092172073	21.107488564245607
13	28.893371671157812	22.326549660314544	25.473075707167265	23.307002961360375
14	22.9573172698709	20.870601240029334	27.904423545503228	28.267657944596543
15	24.963493760067443	27.54419997032188	25.108766013819682	22.383540255790997
16	22.56451227878509	27.75710823266787	25.195434730714062	24.482944757832982
17	22.66171887937134	26.537401960046363	25.68748938146924	25.113389779113053
18	24.056375527163006	25.418773347326496	27.663987750247856	22.860863375262642
19	26.28664333994998	24.840910215080356	27.475703726324706	21.39674271864496
20	25.5457655987561	24.712950198821908	27.62796539272972	22.113318809692274
21	23.877123974975753	26.089972020843504	27.612373626042768	22.420530378137975
22	23.480555453999987	25.736522799464073	27.585813857962233	23.197107888573708
23	23.195494947192298	25.900720232091512	28.39045654843448	22.51332827228171
24	23.344853319110776	26.214276036637425	27.0320373170118	23.40883332724
25	23.31754084505225	26.001045186015155	28.418521728470996	22.2628922404616
26	23.664968418607753	25.971152005746372	27.899477191933574	22.464402383712304
27	23.71658254281284	25.9357748247808	27.163545804309347	23.184096828097008
28	22.86570219940687	25.870289404695594	28.15658004813017	23.107428347767367
29	24.25197155201521	25.642757140491497	27.187094748477918	22.918176559015375
30	23.90518915501227	25.516302536189027	28.015178853693314	22.56332945510539
31	23.658086535380406	25.45597852852433	27.821948476200514	23.06398645989475
32	22.28945200854214	25.57361571994176	28.22980758684614	23.90712468466996
33	22.662041467647622	25.768996685943108	28.336691835720845	23.232270010688424
34	23.114955407547274	25.598239958364605	28.079696508949674	23.207108125138447
35	23.212269537558953	26.268363337627342	27.545382794001576	22.97398433081213
36	23.820025850073872	25.94609764962182	27.26623640559239	22.967640094711918
37	23.505179692422832	25.60662725354793	27.487531963121707	23.400661090907526
38	23.656366064573568	26.029110366051672	27.004939901804132	23.309583667570628
39	22.950542916068983	25.786631511713182	27.752591996799925	23.510233575417914
40	23.290443429844576	26.31481604941192	27.81958282884111	22.57515769190239
41	23.340982259795393	25.289738036813773	27.689364694648688	23.679915008742142
42	22.445047087135393	26.71149209981311	27.553877618610333	23.28958319444116
43	23.58281593758132	25.560389600614208	27.300108174601977	23.556686287202496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	56.0
2	81.0
3	357.0
4	633.0
5	633.0
6	800.0
7	967.0
8	908.0
9	849.0
10	1135.0
11	1421.0
12	1421.0
13	2345.0
14	3269.0
15	5854.0
16	8439.0
17	9542.5
18	10646.0
19	10646.0
20	8991.0
21	7336.0
22	6678.0
23	6020.0
24	7525.0
25	9030.0
26	9030.0
27	11546.0
28	14062.0
29	17226.5
30	20391.0
31	24199.5
32	28008.0
33	28008.0
34	34750.5
35	41493.0
36	45850.5
37	50208.0
38	54536.0
39	58864.0
40	58864.0
41	63619.5
42	68375.0
43	71067.5
44	73760.0
45	74188.5
46	74617.0
47	74617.0
48	74220.5
49	73824.0
50	74078.0
51	74332.0
52	72002.5
53	69673.0
54	69673.0
55	66713.5
56	63754.0
57	55394.5
58	47035.0
59	42618.0
60	38201.0
61	38201.0
62	34156.0
63	30111.0
64	23708.0
65	17305.0
66	14764.5
67	12224.0
68	12224.0
69	10175.0
70	8126.0
71	6880.5
72	5635.0
73	5427.5
74	5220.0
75	5220.0
76	4193.5
77	3167.0
78	2360.5
79	1554.0
80	1077.0
81	600.0
82	600.0
83	462.0
84	324.0
85	268.0
86	212.0
87	156.5
88	101.0
89	101.0
90	84.0
91	67.0
92	39.0
93	11.0
94	6.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	929978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.24965648375391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.53295595813935	39.09620174620244
2	8.45152361325985	7.817601277552052
3	2.218171616752168	3.0776902589040236
4	1.0251285538999086	1.896473738782327
5	0.6290498800469444	1.4546670431658888
6	0.41471982537561547	1.150838967637477
7	0.3077756339141102	0.9964162139818038
8	0.24257349873988157	0.8975152791025466
9	0.20367891990813264	0.8478072070859843
>10	1.5763705895723905	14.919294971199944
>50	0.2219545213526499	7.2421277140061635
>100	0.1609695596457195	13.995543326702172
>500	0.011582244379234243	3.4271163373241813
>1k	0.0033092126797812124	2.3935918914706353
>5k	2.3637233427008662E-4	0.787114026882328
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7200	0.7742118630763308	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4822	0.5185068894102871	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2857	0.3072115684457052	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1504	0.16172425584261133	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1472	0.15828331422893877	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1268	0.13634731144177603	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1182	0.12709978085503099	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1170	0.12580942774990375	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1154	0.12408895694306747	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1145	0.12312119211422205	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1117	0.12011036820225855	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1065	0.11451883808004061	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1056	0.11355107325119519	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1055	0.1134435438257679	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1028	0.11054024933923168	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	982	0.10559389576957734	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	975	0.10484118979158646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.3011770170907267E-4	0.0	0.0
2	0.0	0.0	4.3011770170907267E-4	0.0	0.0
3	0.0	0.0	6.45176552563609E-4	0.0	0.0
4	0.0	0.0	8.602354034181453E-4	1.0752942542726817E-4	0.0
5	0.0	0.0	0.0015054119559817544	1.0752942542726817E-4	0.0
6	0.0	0.0	0.002903294486536241	1.0752942542726817E-4	0.0
7	1.0752942542726817E-4	0.0	0.0076345892053360405	3.225882762818045E-4	0.0
8	2.1505885085453634E-4	0.0	0.009355060012172331	3.225882762818045E-4	0.0
9	2.1505885085453634E-4	0.0	0.011075530819008621	0.0022581179339726315	0.0
10	4.3011770170907267E-4	0.0	0.012258354498708572	0.0022581179339726315	0.0
11	4.3011770170907267E-4	0.0	0.01817247289720832	0.0024731767848271678	0.0
12	5.376471271363409E-4	0.0	0.01957035542776281	0.002903294486536241	0.0
13	5.376471271363409E-4	0.0	0.020753179107462758	0.002903294486536241	0.0
14	5.376471271363409E-4	0.0	0.021828473361735438	0.0030108239119635088	0.0
15	6.45176552563609E-4	0.0	0.024301650146562607	0.0030108239119635088	0.0
16	6.45176552563609E-4	0.0	0.026237179804253434	0.0030108239119635088	0.0
17	6.45176552563609E-4	0.0	0.02645223865510797	0.0030108239119635088	0.0
18	6.45176552563609E-4	0.0	0.026882356356817044	0.0030108239119635088	0.0
19	6.45176552563609E-4	0.0	0.027420003483953383	0.003118353337390777	0.0
20	6.45176552563609E-4	0.0	0.02752753290938065	0.0034409416136725814	0.0
21	6.45176552563609E-4	0.0	0.02828023888737153	0.003763529889954386	0.0
22	6.45176552563609E-4	0.0	0.028387768312798798	0.00408611816623619	0.0
23	6.45176552563609E-4	0.0	0.02881788601450787	0.004516235867945263	0.0
24	6.45176552563609E-4	0.0	0.029032944865362405	0.0054840006967906765	0.0
25	6.45176552563609E-4	0.0	0.029032944865362405	0.006129177249354286	0.0
26	6.45176552563609E-4	0.0	0.029032944865362405	0.006666824376490627	0.0
27	6.45176552563609E-4	0.0	0.029032944865362405	0.008494824608754186	0.0
28	7.527059779908772E-4	0.0	0.029032944865362405	0.01817247289720832	0.0
29	7.527059779908772E-4	0.0	0.029140474290789673	0.04064612281150737	0.0
30	7.527059779908772E-4	0.0	0.029140474290789673	0.06903389112430616	0.0
31	7.527059779908772E-4	0.0	0.029140474290789673	0.13903554707745774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1225	0.0	25.06939	1
GTATCAA	3440	0.0	23.501453	1
ACGGTGT	40	0.0019309118	23.125	35
CCGCTCT	310	0.0	20.887096	28
CTGATCG	55	5.142081E-4	20.181818	8
TTAACGG	300	0.0	19.733334	35
ATTAGAG	350	0.0	19.557144	3
AGCTTCG	340	0.0	19.044117	21
GCCGGCA	350	0.0	19.028572	15
GCCGCTC	355	0.0	18.760563	27
TTCGCCG	355	0.0	18.760563	24
GTATTAG	510	0.0	18.5	1
TCGGGTA	50	0.007033896	18.5	25
CGCTCTC	340	0.0	18.5	29
ACGGTAC	200	0.0	18.5	3
TGCTCGC	375	0.0	18.253334	10
AACGGCC	335	0.0	18.22388	37
CGCCGGC	395	0.0	17.797468	14
TATTAGA	355	0.0	17.71831	2
TGTCCGG	285	0.0	17.526318	14
>>END_MODULE