FastQCFastQC Report
Fri 10 Feb 2017
ERR1633142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458484
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12950.2824526046710463No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT11360.2477730956805472No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10350.22574397361739992No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC10320.22508964325908865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.21047626525680288No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8930.19477233665733154No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8000.17448809554968114No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7830.1707802235192504No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7830.1707802235192504No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC7310.15943849730852114No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA7110.1550762949197791No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT6980.15224086336709677No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC6780.14787866097835475No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG6390.13937236632030778No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA6290.1371912651259368No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA5900.12868497046788982No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT5670.12366843772083648No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATGTTTTCAAAC5590.12192355676533968No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG5280.11516214306278953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5140.11210860139067012No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5050.1101456103157362No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG4990.10883694959911361No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT4950.10796450912136521No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC4850.10578340792699419No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA4810.10491096744924576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATC200.001840781937.04
GGTATCA2900.024.2413791
ATTTCGG705.0908893E-621.14285929
GTATCAA6550.020.618321
GGCCGTT450.003822353720.55555514
GCCGTTA450.003822353720.55555515
GACTCCG450.003822353720.55555537
TAATGGG555.136613E-420.181824
TAACGGC851.2419696E-619.58823636
AACGGCC851.2419696E-619.58823637
TTAACGG851.2419696E-619.58823635
AGCTTCG701.2171695E-418.521
GCTTCGC701.2171695E-418.522
AATTGTA1306.9303496E-1018.55
TATTAGC609.225274E-418.52
GAAGTGT609.225274E-418.56
GACGTTA500.00702859118.49999820
GTGACAC1002.8655631E-718.49999824
TCGCCAT2050.018.0487813
TTGGCCG2100.017.6190531
Produced by FastQC (version 0.11.5)