FastQCFastQC Report
Fri 10 Feb 2017
ERR1633141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319924
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9430.2947575049074155No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7240.22630374713994575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.2197396881759418No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6200.19379602655630712No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6060.18941998724697115No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5500.1719158300096273No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5160.1612883059726685No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5120.16003800902714393No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4440.1387829609532264No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4060.12690513997074304No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4010.12534226878883736No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4000.1250296945524562No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA3940.12315424913416936No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3870.12096622947950138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3830.1197159325339768No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG3760.11752791287930883No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT3490.10908840849701805No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT3310.1034620722421575No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG3290.10283692376939522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG352.3829627E-531.7142851
GCACTAC303.5937247E-430.83333413
GGTATCA2000.028.6750011
TAATGTG405.926942E-527.755
GTATCAA5100.023.2156871
TGCACTA450.00381986620.55555512
CACTAGG555.13195E-420.18181826
ATTATAC555.13195E-420.1818183
AAAGCTA555.13195E-420.1818185
GTATTAG759.231535E-619.7333341
GTTTGCA609.2169654E-418.511
TACCCTC1002.858833E-718.55
GACGCCT500.00702405818.526
TCACGTA500.00702405818.525
TTTATTC1552.0008883E-1117.9032273
AGATCGG953.5899538E-617.52631619
GTACTGT953.5899538E-617.5263166
GTTCTAG852.7131815E-517.4117641
CAACCCA852.7131815E-517.41176417
GTCAACC752.0613958E-417.26666615
Produced by FastQC (version 0.11.5)