##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633141.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319924
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719545892149384	33.0	33.0	33.0	27.0	33.0
2	31.249284204998688	33.0	33.0	33.0	27.0	33.0
3	31.678433002838172	33.0	33.0	33.0	27.0	33.0
4	31.809204686112952	33.0	33.0	33.0	27.0	33.0
5	31.906462159762945	33.0	33.0	33.0	33.0	33.0
6	34.84144046711094	37.0	37.0	37.0	27.0	37.0
7	35.0223709380978	37.0	37.0	37.0	33.0	37.0
8	35.14427801602881	37.0	37.0	37.0	33.0	37.0
9	35.23772833547967	37.0	37.0	37.0	33.0	37.0
10	35.28720883709881	37.0	37.0	37.0	33.0	37.0
11	35.2942667633563	37.0	37.0	37.0	33.0	37.0
12	35.26939523136745	37.0	37.0	37.0	33.0	37.0
13	35.28638676685713	37.0	37.0	37.0	33.0	37.0
14	35.264184618846976	37.0	37.0	37.0	33.0	37.0
15	35.273677498405874	37.0	37.0	37.0	33.0	37.0
16	35.2778378614921	37.0	37.0	37.0	33.0	37.0
17	35.232189520011005	37.0	37.0	37.0	33.0	37.0
18	35.24282329553269	37.0	37.0	37.0	33.0	37.0
19	35.245414535952285	37.0	37.0	37.0	33.0	37.0
20	35.244142358810215	37.0	37.0	37.0	33.0	37.0
21	35.24457996274115	37.0	37.0	37.0	33.0	37.0
22	35.25737362623623	37.0	37.0	37.0	33.0	37.0
23	35.237343869168924	37.0	37.0	37.0	33.0	37.0
24	35.241426088696066	37.0	37.0	37.0	33.0	37.0
25	35.20097585676598	37.0	37.0	37.0	33.0	37.0
26	35.184709493504705	37.0	37.0	37.0	33.0	37.0
27	35.13364111476476	37.0	37.0	37.0	33.0	37.0
28	35.10792563233768	37.0	37.0	37.0	33.0	37.0
29	35.156424650854575	37.0	37.0	37.0	33.0	37.0
30	35.10477488403496	37.0	37.0	37.0	33.0	37.0
31	35.10454357910004	37.0	37.0	37.0	33.0	37.0
32	35.13838286593066	37.0	37.0	37.0	33.0	37.0
33	35.114786636826246	37.0	37.0	37.0	33.0	37.0
34	35.05137157574924	37.0	37.0	37.0	33.0	37.0
35	35.046986159212814	37.0	37.0	37.0	27.0	37.0
36	35.02314299646166	37.0	37.0	37.0	27.0	37.0
37	35.05024005701354	37.0	37.0	37.0	27.0	37.0
38	34.901673522461586	37.0	37.0	37.0	27.0	37.0
39	34.958590165164225	37.0	37.0	37.0	27.0	37.0
40	35.0003625861142	37.0	37.0	37.0	27.0	37.0
41	35.029650792063116	37.0	37.0	37.0	27.0	37.0
42	34.945518310598764	37.0	37.0	37.0	27.0	37.0
43	34.732527100186296	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	19.0
17	9.0
18	4.0
19	16.0
20	30.0
21	59.0
22	172.0
23	424.0
24	864.0
25	1546.0
26	2718.0
27	3958.0
28	5591.0
29	7366.0
30	9850.0
31	12593.0
32	15832.0
33	20918.0
34	29530.0
35	54091.0
36	154324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.761005738862984	21.554494192370687	14.112414198372115	24.57208587039422
2	15.214238381615633	23.755016816493917	39.03864667858616	21.992098123304284
3	17.325364774133856	27.21177529663295	31.764731623760646	23.698128305472547
4	12.69207686825621	18.2959077780973	39.21493854790513	29.797076805741362
5	13.144996936772483	38.2275165351771	35.946974906540305	12.68051162151011
6	28.697753216388893	40.135469674047584	18.017716707718083	13.14906040184544
7	25.040947224965933	33.1278678686188	24.275452920068517	17.555731986346757
8	23.11767794851277	37.41732411447719	21.30912341681149	18.155874520198548
9	24.957802478088546	16.206974156362136	20.559570397969516	38.2756529675798
10	14.10366211975344	29.0887835861017	34.829209437241346	21.978344856903515
11	32.247658818969505	24.410484990185168	25.291006614070845	18.050849576774482
12	21.284117477901003	26.706342756404645	30.90296445405784	21.106575311636515
13	26.703529588277213	22.71258173816281	27.711268926370014	22.872619747189958
14	21.172528475512934	21.863630112151636	28.108550780810443	28.855290631524987
15	23.069854090346457	29.57139820707418	25.344144234255634	22.014603468323727
16	22.67569797826984	29.62859929233193	25.089708805841386	22.605993923556845
17	21.90051387204461	28.662744901914206	26.651954839274328	22.784786386766857
18	21.339755691976844	28.761518360610644	28.202323051724786	21.696402895687726
19	23.810029882096998	27.396819244570587	28.371738287843364	20.421412585489055
20	23.483702379315087	26.664457808729576	28.84528825596079	21.006551555994548
21	21.742976456908515	28.488328478013532	27.415886272989837	22.352808792088123
22	21.6338880484115	28.092609494755006	27.432140133281653	22.841362323551845
23	21.351633512959328	28.350483239769446	28.227328990635275	22.07055425663595
24	22.92200647653818	28.397369375226617	26.847626311248924	21.832997836986284
25	22.85136469911604	27.440579637663948	27.79722684137483	21.91082882184519
26	22.372188394743752	27.766281991973095	27.50872082119503	22.352808792088123
27	22.254660481864445	27.64062714894788	27.289293707255474	22.81541866193221
28	21.34944549330466	28.08354484189995	28.359547892624498	22.20746177217089
29	22.815106087695828	28.230142158762707	27.249284204998688	21.705467548542778
30	21.965216738975506	28.563346294745003	27.507783098485888	21.9636538677936
31	22.552856303372053	27.892249409234694	27.711894074842775	21.843000212550482
32	21.392580737925257	27.934759505382527	27.997274352658756	22.675385404033456
33	21.7332866555807	27.560295570197923	28.326102449331714	22.38031532488966
34	21.830809817331616	27.706580312824297	27.953201385329013	22.509408484515074
35	22.244345532063864	28.314224628349233	27.375876770733047	22.065553068853852
36	22.324364536577438	28.246396019054526	27.237406384016204	22.191833060351833
37	22.82948450256936	27.028294219877218	27.514659731686276	22.627561545867145
38	22.91638014028332	27.780347832610246	27.640939723184253	21.662332303922184
39	22.241219789700054	27.574986559307835	28.14480939223066	22.038984258761456
40	22.253097610682538	27.48058913992073	28.043535339643167	22.222777909753567
41	21.645765869393983	27.701579125042198	28.24170740550881	22.41094760005501
42	22.463460071767045	27.462772408447005	27.539040522124004	22.534726997661945
43	22.059926732598992	27.15676223102987	27.981645640839698	22.80166539553144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	8.5
2	10.0
3	55.0
4	100.0
5	100.0
6	128.5
7	157.0
8	152.5
9	148.0
10	208.5
11	269.0
12	269.0
13	411.0
14	553.0
15	1131.5
16	1710.0
17	1972.0
18	2234.0
19	2234.0
20	2320.5
21	2407.0
22	3052.5
23	3698.0
24	4665.5
25	5633.0
26	5633.0
27	6781.0
28	7929.0
29	10431.5
30	12934.0
31	14013.0
32	15092.0
33	15092.0
34	16541.0
35	17990.0
36	19412.5
37	20835.0
38	22695.0
39	24555.0
40	24555.0
41	26021.0
42	27487.0
43	25813.0
44	24139.0
45	24333.5
46	24528.0
47	24528.0
48	23947.5
49	23367.0
50	23670.0
51	23973.0
52	23957.5
53	23942.0
54	23942.0
55	20265.5
56	16589.0
57	14350.5
58	12112.0
59	10415.0
60	8718.0
61	8718.0
62	7684.5
63	6651.0
64	5445.5
65	4240.0
66	3539.5
67	2839.0
68	2839.0
69	2344.0
70	1849.0
71	1594.5
72	1340.0
73	1127.5
74	915.0
75	915.0
76	660.0
77	405.0
78	327.5
79	250.0
80	191.5
81	133.0
82	133.0
83	114.0
84	95.0
85	77.5
86	60.0
87	39.0
88	18.0
89	18.0
90	14.5
91	11.0
92	6.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	319924.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.712447352359796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67639172538532	50.29055197123149
2	5.840907800578659	6.625043522606096
3	1.6381919079722973	2.787176170018223
4	0.8435897071310323	1.9136814741064518
5	0.480834236819819	1.3634643170428042
6	0.33604349644281867	1.1434709460069756
7	0.2754981455430317	1.0936921852347359
8	0.19335518190946074	0.8772516459476994
9	0.16325316024897446	0.833263763015369
>10	1.2705454439990664	14.939937000000663
>50	0.178965730019756	7.108456718043109
>100	0.09801815367235865	9.408196427720178
>500	0.0044053102774093775	1.6158138590262299
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	943	0.2947575049074155	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	724	0.22630374713994575	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	703	0.2197396881759418	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	620	0.19379602655630712	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	606	0.18941998724697115	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	550	0.1719158300096273	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	516	0.1612883059726685	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	512	0.16003800902714393	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	444	0.1387829609532264	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	406	0.12690513997074304	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	401	0.12534226878883736	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	400	0.1250296945524562	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	394	0.12315424913416936	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	387	0.12096622947950138	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	383	0.1197159325339768	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	376	0.11752791287930883	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	349	0.10908840849701805	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	331	0.1034620722421575	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	329	0.10283692376939522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	3.125742363811405E-4	0.0	0.0
7	0.0	0.0	9.377227091434216E-4	0.0	0.0
8	0.0	0.0	9.377227091434216E-4	0.0	0.0
9	0.0	0.0	0.0015628711819057027	0.0	0.0
10	0.0	0.0	0.0018754454182868432	0.0	0.0
11	0.0	0.0	0.004376039309335967	0.0	0.0
12	0.0	0.0	0.004688613545717108	0.0	0.0
13	0.0	0.0	0.005313762018479389	0.0	0.0
14	0.0	0.0	0.00593891049124167	0.0	0.0
15	0.0	0.0	0.00593891049124167	0.0	0.0
16	0.0	0.0	0.006564058964003951	0.0	0.0
17	0.0	0.0	0.006564058964003951	0.0	0.0
18	0.0	0.0	0.006564058964003951	3.125742363811405E-4	0.0
19	0.0	0.0	0.006564058964003951	3.125742363811405E-4	0.0
20	0.0	0.0	0.006564058964003951	3.125742363811405E-4	0.0
21	0.0	0.0	0.007189207436766232	3.125742363811405E-4	0.0
22	0.0	0.0	0.007501781673147373	3.125742363811405E-4	0.0
23	0.0	0.0	0.007501781673147373	3.125742363811405E-4	0.0
24	0.0	0.0	0.007501781673147373	3.125742363811405E-4	0.0
25	0.0	0.0	0.007501781673147373	9.377227091434216E-4	0.0
26	0.0	0.0	0.007501781673147373	9.377227091434216E-4	0.0
27	0.0	0.0	0.007501781673147373	0.0015628711819057027	0.0
28	0.0	0.0	0.007501781673147373	0.006876633200385091	0.0
29	0.0	0.0	0.007501781673147373	0.01812930571010615	0.0
30	0.0	0.0	0.007501781673147373	0.029381978219827208	0.0
31	0.0	0.0	0.007501781673147373	0.07439266825871145	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAG	35	2.3829627E-5	31.714285	1
GCACTAC	30	3.5937247E-4	30.833334	13
GGTATCA	200	0.0	28.675001	1
TAATGTG	40	5.926942E-5	27.75	5
GTATCAA	510	0.0	23.215687	1
TGCACTA	45	0.003819866	20.555555	12
CACTAGG	55	5.13195E-4	20.181818	26
ATTATAC	55	5.13195E-4	20.181818	3
AAAGCTA	55	5.13195E-4	20.181818	5
GTATTAG	75	9.231535E-6	19.733334	1
GTTTGCA	60	9.2169654E-4	18.5	11
TACCCTC	100	2.858833E-7	18.5	5
GACGCCT	50	0.007024058	18.5	26
TCACGTA	50	0.007024058	18.5	25
TTTATTC	155	2.0008883E-11	17.903227	3
AGATCGG	95	3.5899538E-6	17.526316	19
GTACTGT	95	3.5899538E-6	17.526316	6
GTTCTAG	85	2.7131815E-5	17.411764	1
CAACCCA	85	2.7131815E-5	17.411764	17
GTCAACC	75	2.0613958E-4	17.266666	15
>>END_MODULE