FastQCFastQC Report
Fri 10 Feb 2017
ERR1633140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424818
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60501.424139278467485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48861.150139589188782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23480.5527072769986205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.30460102914659926No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11100.26128836348742285No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10880.25610967520208655No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10550.24834164277408205No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9880.2325701829960124No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9060.21326779938703164No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8450.1989087091413264No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7720.1817248798308923No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7670.18054790522058858No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7580.1784293509220419No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7450.17536921693525226No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7250.17066131849403746No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7230.17019052864991596No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7170.1687781591175515No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7090.16689499974106559No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7040.1657180251307619No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6960.16383486575427597No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.15700841301451446No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6650.15653762317039296No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6590.1551252536380285No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6420.15112353996299593No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6390.15041735519681368No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5990.14100155831438405No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5890.13864760909377663No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5890.13864760909377663No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5820.13699984463935144No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5800.13652905479522995No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5780.13605826495110848No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5740.13511668526286552No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5680.13370431573050107No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5670.13346892080844033No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5540.1304087868216507No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5340.12570088838043586No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5100.12005141025097806No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5090.11981601532891732No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5070.11934522548479586No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4970.11699127626418843No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4950.11652048642006696No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4950.11652048642006696No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4940.1162850914980062No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4880.11487272196564176No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4880.11487272196564176No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4820.1134603524332773No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4810.11322495751121657No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4790.11275416766709509No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4670.10992942860236618No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4650.10945863875824471No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4630.10898784891412322No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4570.10757547938175878No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4510.10616310984939432No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4500.10592771492733358No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4480.10545692508321211No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4460.10498613523909062No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4430.10427995047290839No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4430.10427995047290839No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC4370.10286758094054395No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.10263218601848322No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4360.10263218601848322No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4330.10192600125230099No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4320.10169060633024025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGAT200.001840630537.035
CTCGTAT401.5969254E-632.37536
GGACCGA303.5956924E-430.8333346
CGTGCCT250.00549249229.613
AGCCCGC250.00549249229.618
TAAGTTC250.00549249229.630
CCGCTCG250.00549249229.621
GCGTGCC250.00549249229.612
GCGTGAT250.00549249229.636
ATAAAGC250.00549249229.63
GGTATCA7700.028.8311671
TCGTATG454.0004415E-628.77777737
ACATATA556.246137E-726.909091
CCTACAT358.8601536E-426.4285721
GTATTAT659.362702E-825.6153851
GTATCAA24450.025.1206551
TTATAGA601.3340923E-624.6666684
ATCTCGT551.8990002E-523.54545434
ATCTCCG551.8990002E-523.5454547
CGAGCCC551.8990002E-523.54545412
Produced by FastQC (version 0.11.5)