##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633140.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424818
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.740462974732708	33.0	33.0	33.0	27.0	33.0
2	31.231336713604414	33.0	33.0	33.0	27.0	33.0
3	31.67819395599998	33.0	33.0	33.0	27.0	33.0
4	31.806973809960972	33.0	33.0	33.0	27.0	33.0
5	31.928891901943892	33.0	33.0	33.0	33.0	33.0
6	34.734639304360925	37.0	37.0	37.0	27.0	37.0
7	34.93353624375615	37.0	37.0	37.0	27.0	37.0
8	35.11589668987661	37.0	37.0	37.0	33.0	37.0
9	35.20207477084304	37.0	37.0	37.0	33.0	37.0
10	35.17981347306376	37.0	37.0	37.0	33.0	37.0
11	35.2200377573455	37.0	37.0	37.0	33.0	37.0
12	35.164413937262545	37.0	37.0	37.0	33.0	37.0
13	35.190144956193	37.0	37.0	37.0	33.0	37.0
14	35.15023139320839	37.0	37.0	37.0	33.0	37.0
15	35.191390195330705	37.0	37.0	37.0	33.0	37.0
16	35.20524083254476	37.0	37.0	37.0	33.0	37.0
17	35.183596269461276	37.0	37.0	37.0	33.0	37.0
18	35.17992410867713	37.0	37.0	37.0	33.0	37.0
19	35.166546615256415	37.0	37.0	37.0	33.0	37.0
20	35.17469834140738	37.0	37.0	37.0	33.0	37.0
21	35.16463050059084	37.0	37.0	37.0	33.0	37.0
22	35.16407026067634	37.0	37.0	37.0	33.0	37.0
23	35.162617874007225	37.0	37.0	37.0	33.0	37.0
24	35.16133967958043	37.0	37.0	37.0	33.0	37.0
25	35.14709357889732	37.0	37.0	37.0	33.0	37.0
26	35.11410533451972	37.0	37.0	37.0	33.0	37.0
27	35.04259471114689	37.0	37.0	37.0	27.0	37.0
28	35.01413781901897	37.0	37.0	37.0	27.0	37.0
29	35.041912065872914	37.0	37.0	37.0	27.0	37.0
30	34.97286838128328	37.0	37.0	37.0	27.0	37.0
31	34.95039758202336	37.0	37.0	37.0	27.0	37.0
32	34.96476373411673	37.0	37.0	37.0	27.0	37.0
33	34.95634836565306	37.0	37.0	37.0	27.0	37.0
34	34.844858268717424	37.0	37.0	37.0	27.0	37.0
35	34.80657128464425	37.0	37.0	37.0	27.0	37.0
36	34.77694918765212	37.0	37.0	37.0	27.0	37.0
37	34.767232085269455	37.0	37.0	37.0	27.0	37.0
38	34.59964267050831	37.0	37.0	37.0	27.0	37.0
39	34.645262206403686	37.0	37.0	37.0	27.0	37.0
40	34.63154809824442	37.0	37.0	37.0	27.0	37.0
41	34.60067134631772	37.0	37.0	37.0	27.0	37.0
42	34.44508001073401	37.0	37.0	37.0	27.0	37.0
43	34.191976328686636	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	35.0
17	25.0
18	22.0
19	20.0
20	39.0
21	94.0
22	237.0
23	627.0
24	1314.0
25	2405.0
26	3957.0
27	5828.0
28	8196.0
29	11009.0
30	14298.0
31	18001.0
32	22564.0
33	29294.0
34	40817.0
35	71978.0
36	194043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51581147691482	19.772702663258148	13.960331247734322	22.75115461209271
2	16.75988305580272	23.825732431299993	38.411037197105586	21.003347315791704
3	17.935680691496124	28.951692254094695	30.24495195589641	22.867675098512773
4	11.695832097509992	18.359391551205455	40.58914641093362	29.355629940350923
5	13.770602940553367	36.82730016148092	34.14732897381938	15.25476792414634
6	26.462861743146476	42.4876064573535	16.446572414539872	14.60295938496015
7	25.428536455611578	33.19468572423956	22.686891798370127	18.689886021778737
8	24.353487846560174	34.14638739413113	19.92194304384466	21.578181715464034
9	27.389376156377555	13.506254443079154	19.558493284182873	39.54587611636042
10	16.91971620788196	26.614926862797716	32.203202312519714	24.262154616800608
11	34.39896614550231	24.62819372060506	22.39594367470305	18.57689645918958
12	24.292991351590562	27.10266514130757	27.78436883559548	20.819974671506387
13	29.346449538390555	22.842723236774336	25.415589734898237	22.395237489936868
14	23.32339966762237	21.193546412816783	28.770908953952045	26.712144965608804
15	25.81999821099859	26.8962237946603	26.807009119199282	20.476768875141826
16	21.62172977604527	27.970330824023463	26.077520255733045	24.33041914419822
17	21.98706269508354	25.686058500346032	25.87649299229317	26.450385812277254
18	23.884581161815177	24.712935892546923	29.00088979280539	22.401593152832508
19	26.65070689095095	23.965321620082012	28.940157902913715	20.443813586053324
20	25.65828189954286	23.779595026576086	29.25488091370893	21.30724216017212
21	22.8441356063067	26.289846475431833	29.056207599489664	21.809810318771802
22	22.591321460013464	25.92074723764059	29.108229877265085	22.37970142508086
23	22.461148068113875	26.102472117471482	30.251072223869986	21.185307590544657
24	22.932408702079478	26.310561228573174	27.720106021872898	23.036924047474447
25	22.818948349646202	26.194276137075175	30.411376165793353	20.575399347485277
26	22.838015338333122	26.549251679542767	28.995711104520055	21.617021877604056
27	23.316337819960548	26.03891548851508	28.76690724027701	21.877839451247358
28	21.739898026919764	25.937931066951027	29.971658451383888	22.350512454745324
29	23.90741446925507	26.055863922903455	28.039772326031382	21.996949281810092
30	23.625646747548362	25.289888846517805	29.717667330480346	21.36679707545349
31	22.747152898417674	25.70795022809768	29.10917145695333	22.435725416531316
32	20.807969530481287	25.572362752990692	29.971187661539766	23.648480054988255
33	21.136110051833963	25.58413249909373	30.513772956889774	22.765984492182533
34	21.95834451459213	25.51798652599466	30.20375784453578	22.31991111487743
35	22.045440635754606	26.73709682734724	28.81657556883183	22.400886968066324
36	22.58755514126049	26.57796986003418	28.768319609809378	22.06615538889595
37	22.508697842370147	25.707714833175622	29.01713204242758	22.76645528202666
38	22.277304633984436	26.107650805756816	28.36626508292963	23.248779477329116
39	21.53533983964898	25.904034198174276	29.396117866945374	23.16450809523137
40	21.660805333107355	27.13797437961668	29.785931857877962	21.415288429398004
41	22.62521832879021	25.263995405091123	28.9342730298622	23.17651323625647
42	21.035596420114025	27.50942756662853	28.983705963494955	22.471270049762488
43	22.582611847897216	25.617087788182236	28.741719983616516	23.058580380304036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	78.5
2	98.0
3	357.5
4	617.0
5	617.0
6	811.5
7	1006.0
8	980.0
9	954.0
10	1305.0
11	1656.0
12	1656.0
13	2542.0
14	3428.0
15	5775.5
16	8123.0
17	8405.0
18	8687.0
19	8687.0
20	6557.5
21	4428.0
22	3189.0
23	1950.0
24	2015.0
25	2080.0
26	2080.0
27	2498.5
28	2917.0
29	4021.0
30	5125.0
31	7239.0
32	9353.0
33	9353.0
34	12895.0
35	16437.0
36	18565.0
37	20693.0
38	23532.5
39	26372.0
40	26372.0
41	29738.5
42	33105.0
43	35850.5
44	38596.0
45	38380.0
46	38164.0
47	38164.0
48	38778.5
49	39393.0
50	39481.5
51	39570.0
52	37261.5
53	34953.0
54	34953.0
55	32166.5
56	29380.0
57	24141.0
58	18902.0
59	17254.5
60	15607.0
61	15607.0
62	12894.0
63	10181.0
64	7359.0
65	4537.0
66	4140.0
67	3743.0
68	3743.0
69	2966.0
70	2189.0
71	1658.0
72	1127.0
73	1049.0
74	971.0
75	971.0
76	591.0
77	211.0
78	147.0
79	83.0
80	66.0
81	49.0
82	49.0
83	47.5
84	46.0
85	30.0
86	14.0
87	11.5
88	9.0
89	9.0
90	7.0
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	424818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.948356141410372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6271698947574	17.632496863237783
2	9.366990736467642	4.486480602604378
3	3.9937330679278333	2.8693002553338967
4	2.2394119580816527	2.145209404778903
5	1.5687978584860645	1.8785064914453091
6	1.1920804688689417	1.7129020570615727
7	0.8763236367216635	1.4690557383142424
8	0.6794755404846139	1.3017857786322269
9	0.5805518935427039	1.251293715461775
>10	4.72335421657053	22.86572353259883
>50	0.6155248989627404	10.22787237977914
>100	0.4976159085770206	22.827196416833758
>500	0.03197531942663586	5.0652388728103
>1k	0.005995372392494224	2.8191798157782464
>5k	9.992287320823706E-4	1.4477580753296317
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6050	1.424139278467485	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4886	1.150139589188782	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2348	0.5527072769986205	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1294	0.30460102914659926	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1110	0.26128836348742285	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1088	0.25610967520208655	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1055	0.24834164277408205	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	988	0.2325701829960124	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	906	0.21326779938703164	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	845	0.1989087091413264	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	772	0.1817248798308923	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	767	0.18054790522058858	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	758	0.1784293509220419	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	745	0.17536921693525226	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	725	0.17066131849403746	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	723	0.17019052864991596	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	717	0.1687781591175515	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	709	0.16689499974106559	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	704	0.1657180251307619	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	696	0.16383486575427597	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.15700841301451446	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	665	0.15653762317039296	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	659	0.1551252536380285	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	642	0.15112353996299593	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	639	0.15041735519681368	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	599	0.14100155831438405	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	589	0.13864760909377663	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	589	0.13864760909377663	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	582	0.13699984463935144	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	580	0.13652905479522995	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	578	0.13605826495110848	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	574	0.13511668526286552	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	568	0.13370431573050107	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	567	0.13346892080844033	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	554	0.1304087868216507	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	534	0.12570088838043586	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	510	0.12005141025097806	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	509	0.11981601532891732	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	507	0.11934522548479586	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	497	0.11699127626418843	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	495	0.11652048642006696	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	495	0.11652048642006696	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	494	0.1162850914980062	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	488	0.11487272196564176	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	488	0.11487272196564176	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	482	0.1134603524332773	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	481	0.11322495751121657	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	479	0.11275416766709509	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	467	0.10992942860236618	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	465	0.10945863875824471	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	463	0.10898784891412322	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	457	0.10757547938175878	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	451	0.10616310984939432	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	450	0.10592771492733358	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	448	0.10545692508321211	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	446	0.10498613523909062	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	443	0.10427995047290839	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	443	0.10427995047290839	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	437	0.10286758094054395	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.10263218601848322	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	436	0.10263218601848322	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	433	0.10192600125230099	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	432	0.10169060633024025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0014123695323644478	0.0	0.0
2	4.707898441214826E-4	0.0	0.002353949220607413	2.353949220607413E-4	0.0
3	4.707898441214826E-4	0.0	0.0035309238309111195	2.353949220607413E-4	0.0
4	4.707898441214826E-4	0.0	0.0035309238309111195	4.707898441214826E-4	0.0
5	7.061847661822239E-4	0.0	0.006591057817700757	4.707898441214826E-4	0.0
6	7.061847661822239E-4	0.0	0.01647764454425189	4.707898441214826E-4	0.0
7	7.061847661822239E-4	0.0	0.03271989416644304	4.707898441214826E-4	0.0
8	7.061847661822239E-4	0.0	0.03860476721796158	4.707898441214826E-4	0.0
9	7.061847661822239E-4	0.0	0.047078984412148264	9.415796882429652E-4	0.0
10	0.0032955289088503785	0.0	0.053905437151909756	9.415796882429652E-4	0.0
11	0.0035309238309111195	0.0	0.07650334966974093	0.0014123695323644478	0.0
12	0.0035309238309111195	0.0	0.08238822272125945	0.0014123695323644478	0.0
13	0.0035309238309111195	0.0	0.08874388561689947	0.0014123695323644478	0.0
14	0.0035309238309111195	0.0	0.09486415359047874	0.0016477644544251892	0.0
15	0.0035309238309111195	0.0	0.10475074031702988	0.0016477644544251892	0.0
16	0.0035309238309111195	0.0	0.1139311422773988	0.0016477644544251892	0.0
17	0.0035309238309111195	0.0	0.11769746103037065	0.0016477644544251892	0.0
18	0.0035309238309111195	0.0	0.12193456962746399	0.0018831593764859305	0.0
19	0.0035309238309111195	0.0	0.12546549345837513	0.0021185542985466717	0.0
20	0.0035309238309111195	0.0	0.1287610223672255	0.0021185542985466717	0.0
21	0.0035309238309111195	0.0	0.1344105004966833	0.0025893441426681546	0.0
22	0.003766318752971861	0.0	0.13652905479522995	0.004001713675032602	0.0
23	0.003766318752971861	0.0	0.13770602940553367	0.004707898441214826	0.0
24	0.003766318752971861	0.0	0.13864760909377663	0.006120267973579274	0.0
25	0.003766318752971861	0.0	0.13911839893789812	0.006591057817700757	0.0
26	0.003766318752971861	0.0	0.13911839893789812	0.007061847661822239	0.0
27	0.003766318752971861	0.0	0.13935379385995886	0.011298956258915582	0.0
28	0.003766318752971861	0.0	0.13935379385995886	0.03578002815323268	0.0
29	0.003766318752971861	0.0	0.1395891887820196	0.08921467546102095	0.0
30	0.003766318752971861	0.0	0.1395891887820196	0.15300669933948186	0.0
31	0.003766318752971861	0.0	0.1395891887820196	0.3053072139127815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGAGAT	20	0.0018406305	37.0	35
CTCGTAT	40	1.5969254E-6	32.375	36
GGACCGA	30	3.5956924E-4	30.833334	6
CGTGCCT	25	0.005492492	29.6	13
AGCCCGC	25	0.005492492	29.6	18
TAAGTTC	25	0.005492492	29.6	30
CCGCTCG	25	0.005492492	29.6	21
GCGTGCC	25	0.005492492	29.6	12
GCGTGAT	25	0.005492492	29.6	36
ATAAAGC	25	0.005492492	29.6	3
GGTATCA	770	0.0	28.831167	1
TCGTATG	45	4.0004415E-6	28.777777	37
ACATATA	55	6.246137E-7	26.90909	1
CCTACAT	35	8.8601536E-4	26.428572	1
GTATTAT	65	9.362702E-8	25.615385	1
GTATCAA	2445	0.0	25.120655	1
TTATAGA	60	1.3340923E-6	24.666668	4
ATCTCGT	55	1.8990002E-5	23.545454	34
ATCTCCG	55	1.8990002E-5	23.545454	7
CGAGCCC	55	1.8990002E-5	23.545454	12
>>END_MODULE