FastQCFastQC Report
Fri 10 Feb 2017
ERR1633139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399059
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22170.5555569477195101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16360.4099644413482718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8430.21124695846980018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.11000879569186511No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4080.10224052082524139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGACC200.001840496837.03
GGTATCA3750.029.5999981
ATACGGA453.999641E-628.7777794
GTATTAG851.8189894E-1228.2941191
TAATACG405.9305643E-527.752
CACGGTA601.333754E-624.6666682
ACGGTAC601.333754E-624.6666683
CGGACAG400.001928990723.1255
GTATCAA11000.022.7045441
AGATTGG502.6978218E-422.19999914
GCACCGC450.003821497620.55555710
TCGGACA450.003821497620.5555574
CCGTTAA450.003821497620.55555716
ACCATCG1101.7444108E-920.18181818
CGGAGGG555.135012E-420.1818187
AGTGTTC555.135012E-420.1818188
TACGGAG656.88771E-519.9230775
TCACGGT759.2409355E-619.7333341
TCATATA851.2411438E-619.5882362
TCCGCCG609.222417E-418.531
Produced by FastQC (version 0.11.5)