##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399059
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797160319651983	33.0	33.0	33.0	33.0	33.0
2	31.324142545337907	33.0	33.0	33.0	27.0	33.0
3	31.74841314191636	33.0	33.0	33.0	27.0	33.0
4	31.864188002275352	33.0	33.0	33.0	33.0	33.0
5	31.98069458400888	33.0	33.0	33.0	33.0	33.0
6	34.88627245595263	37.0	37.0	37.0	27.0	37.0
7	35.02701104347979	37.0	37.0	37.0	33.0	37.0
8	35.210189470729894	37.0	37.0	37.0	33.0	37.0
9	35.2353511636124	37.0	37.0	37.0	33.0	37.0
10	35.299700044354346	37.0	37.0	37.0	33.0	37.0
11	35.33066789622587	37.0	37.0	37.0	33.0	37.0
12	35.27775592080369	37.0	37.0	37.0	33.0	37.0
13	35.30225355147986	37.0	37.0	37.0	33.0	37.0
14	35.27216276289972	37.0	37.0	37.0	33.0	37.0
15	35.29568810627	37.0	37.0	37.0	33.0	37.0
16	35.29645491017619	37.0	37.0	37.0	33.0	37.0
17	35.29632961542028	37.0	37.0	37.0	33.0	37.0
18	35.257783936711114	37.0	37.0	37.0	33.0	37.0
19	35.290916881964826	37.0	37.0	37.0	33.0	37.0
20	35.177066549056654	37.0	37.0	37.0	33.0	37.0
21	35.25717751009249	37.0	37.0	37.0	33.0	37.0
22	35.292523160735634	37.0	37.0	37.0	33.0	37.0
23	35.26437945266239	37.0	37.0	37.0	33.0	37.0
24	35.25669136643955	37.0	37.0	37.0	33.0	37.0
25	35.27342072224909	37.0	37.0	37.0	33.0	37.0
26	35.188024828408835	37.0	37.0	37.0	33.0	37.0
27	35.1559318296292	37.0	37.0	37.0	33.0	37.0
28	35.141222225284984	37.0	37.0	37.0	33.0	37.0
29	35.18445893965554	37.0	37.0	37.0	33.0	37.0
30	35.1542303268439	37.0	37.0	37.0	33.0	37.0
31	35.140234902608384	37.0	37.0	37.0	33.0	37.0
32	35.169736805835726	37.0	37.0	37.0	33.0	37.0
33	35.13254431049043	37.0	37.0	37.0	33.0	37.0
34	35.080837670620134	37.0	37.0	37.0	33.0	37.0
35	35.07009239235301	37.0	37.0	37.0	33.0	37.0
36	35.04529154836754	37.0	37.0	37.0	27.0	37.0
37	35.08429831177846	37.0	37.0	37.0	33.0	37.0
38	34.92655722587387	37.0	37.0	37.0	27.0	37.0
39	34.974111597533195	37.0	37.0	37.0	27.0	37.0
40	35.00829701873658	37.0	37.0	37.0	27.0	37.0
41	35.03129863002714	37.0	37.0	37.0	27.0	37.0
42	34.88677363497628	37.0	37.0	37.0	27.0	37.0
43	34.715255137711466	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	20.0
17	24.0
18	16.0
19	17.0
20	35.0
21	85.0
22	226.0
23	507.0
24	1051.0
25	1905.0
26	3156.0
27	4872.0
28	6687.0
29	9190.0
30	11820.0
31	15358.0
32	19688.0
33	25609.0
34	37014.0
35	68686.0
36	193080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.821282066060405	20.946777293583153	14.268566803405012	23.96337383695143
2	15.421278557807241	23.937061938209638	39.12579342904182	21.5158660749413
3	17.67132178449803	27.625990141808604	31.3830786926244	23.319609381068965
4	12.460563475576294	18.55966160392323	40.19104944381658	28.788725476683897
5	13.143921074327356	37.64180234000486	35.916493551078915	13.29778303458887
6	27.570359270183104	41.02576310771089	18.21159277199612	13.192284850109884
7	24.979764896920003	33.39656542015091	24.11297577551189	17.5106939074172
8	23.501036187631403	36.43571501958357	21.44269393748794	18.620554855297087
9	25.789419609631658	15.754813198048408	20.632287456240807	37.82347973607912
10	14.329961233802521	28.755397071610965	34.710155641145796	22.204486053440718
11	32.50697265316658	24.651993815450847	24.84244184443904	17.998591686943534
12	21.909542198020844	26.43644172916787	31.342984370732147	20.31103170207914
13	27.489418857863125	23.031431442468406	27.44556569329347	22.033584006374998
14	21.89951861754778	21.890497395122026	28.68473082927587	27.525253158054326
15	23.180782791517043	29.195682843890253	25.568149070688794	22.05538529390391
16	21.89801508047682	29.342277708308796	26.093134097965464	22.666573113248916
17	21.468003478182425	28.20410014559251	27.26739655038478	23.06049982584029
18	22.129058610380923	27.571361628230413	28.80325966836984	21.496320093018827
19	24.19441736685553	26.674752354914936	28.822053881756833	20.3087763964727
20	24.187400860524384	26.336707103460892	28.64288238080083	20.833009655213893
21	22.301965373541254	27.698661100238308	28.05625233361483	21.943121192605606
22	21.73012010755302	27.586898177963658	28.281782894258743	22.40119882022458
23	21.80980757231387	27.504454228572715	28.888460102390873	21.79727809672254
24	22.139583369877638	27.476638792759967	28.36948922339804	22.01428861396435
25	22.33379024154323	27.481901172508326	28.832578641253548	21.351729944694895
26	22.478630979378988	27.443811566710686	28.243693288461102	21.833864165449217
27	22.414480064351388	27.374648861446556	27.972555436664752	22.238315637537305
28	21.72686244389927	27.42677147990648	28.827566851017018	22.01879922517723
29	22.45808263940921	27.735748347988643	28.080058337238352	21.726110675363795
30	22.245332143868453	27.763814373313217	28.541393628511074	21.449459854307257
31	22.505193467632605	27.231061071169925	28.4539378888836	21.80980757231387
32	21.29885555769949	27.250607053092402	28.87217178412215	22.578365605085963
33	21.575255789244196	27.324781548593062	29.178141578062395	21.921821084100344
34	21.765703818232392	27.23532109287098	28.60880220719242	22.39017288170421
35	21.85942429565553	27.96904718349918	28.50230166466613	21.66922685617916
36	22.16288819447751	27.545801498024105	28.450429635718027	21.840880671780365
37	22.627481149403973	26.964433830586454	28.28654409498345	22.121540925026125
38	22.570597330219343	27.394445432880854	28.126417396926268	21.908539839973535
39	21.99749911667197	27.34407694100371	28.454188478395427	22.204235463928892
40	21.76069202799586	27.557829794591775	28.879438879965118	21.802039297447244
41	22.042605228800756	26.956414966208005	28.762162988430283	22.23881681656096
42	22.02756985809116	27.44832217792357	28.32463370078109	22.199474263204188
43	21.814318183526744	26.972452694964904	28.681473165622123	22.531755955886222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	25.0
2	38.0
3	121.0
4	204.0
5	204.0
6	292.0
7	380.0
8	343.0
9	306.0
10	430.0
11	554.0
12	554.0
13	884.0
14	1214.0
15	2244.0
16	3274.0
17	3764.0
18	4254.0
19	4254.0
20	4079.5
21	3905.0
22	4558.0
23	5211.0
24	6613.5
25	8016.0
26	8016.0
27	9537.5
28	11059.0
29	13404.5
30	15750.0
31	17687.0
32	19624.0
33	19624.0
34	21167.5
35	22711.0
36	23968.0
37	25225.0
38	26761.0
39	28297.0
40	28297.0
41	29124.5
42	29952.0
43	29435.0
44	28918.0
45	28992.0
46	29066.0
47	29066.0
48	28370.0
49	27674.0
50	27763.5
51	27853.0
52	28008.5
53	28164.0
54	28164.0
55	24934.5
56	21705.0
57	19355.0
58	17005.0
59	14730.5
60	12456.0
61	12456.0
62	10945.0
63	9434.0
64	7612.5
65	5791.0
66	4956.0
67	4121.0
68	4121.0
69	3339.5
70	2558.0
71	2199.0
72	1840.0
73	1532.5
74	1225.0
75	1225.0
76	876.0
77	527.0
78	428.5
79	330.0
80	248.0
81	166.0
82	166.0
83	129.5
84	93.0
85	84.0
86	75.0
87	65.0
88	55.0
89	55.0
90	34.0
91	13.0
92	8.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	399059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.39662760570999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8102481838332	56.936668592868656
2	5.532479173051437	7.0148104374057665
3	1.4198423917911467	2.700396581135517
4	0.6879144653723098	1.7444582874315766
5	0.4389943915476582	1.3915381980971067
6	0.30221201422283533	1.1495533514193976
7	0.22665769448888848	1.0058533416036595
8	0.18617138168231184	0.9442110204283217
9	0.13486090615311477	0.7694753981361858
>10	1.0875788588351354	14.231696582504302
>50	0.10939344420982818	4.7721572707916025
>100	0.062453711784405126	6.15490516037288
>500	3.9779434257582885E-4	0.21259465091347773
>1k	7.955886851516577E-4	0.9716811268916129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2217	0.5555569477195101	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1636	0.4099644413482718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	843	0.21124695846980018	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.11000879569186511	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	408	0.10224052082524139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.51768535479716E-4	0.0	0.0
2	0.0	0.0	0.001002358047306288	0.0	0.0
3	0.0	0.0	0.0012529475591328602	0.0	0.0
4	0.0	0.0	0.0012529475591328602	0.0	0.0
5	0.0	0.0	0.001503537070959432	0.0	0.0
6	0.0	0.0	0.0025058951182657203	0.0	0.0
7	0.0	0.0	0.005011790236531441	0.0	0.0
8	0.0	0.0	0.007016506331144016	0.0	0.0
9	0.0	0.0	0.008018864378450305	5.01179023653144E-4	0.0
10	5.01179023653144E-4	0.0	0.009271811937583165	7.51768535479716E-4	0.0
11	7.51768535479716E-4	0.0	0.012780065103155172	7.51768535479716E-4	0.0
12	7.51768535479716E-4	0.0	0.014784781197767749	0.001002358047306288	0.0
13	7.51768535479716E-4	0.0	0.015787139245074036	0.001002358047306288	0.0
14	7.51768535479716E-4	0.0	0.016288318268727182	0.001002358047306288	0.0
15	7.51768535479716E-4	0.0	0.017290676316033468	0.001002358047306288	0.0
16	0.001002358047306288	2.50589511826572E-4	0.019044802898819473	0.001002358047306288	0.0
17	0.001002358047306288	2.50589511826572E-4	0.019545981922472616	0.001002358047306288	0.0
18	0.001002358047306288	2.50589511826572E-4	0.020047160946125762	0.001002358047306288	0.0
19	0.001002358047306288	2.50589511826572E-4	0.020297750457952332	0.001002358047306288	0.0
20	0.001002358047306288	2.50589511826572E-4	0.020548339969778905	0.001002358047306288	0.0
21	0.0012529475591328602	2.50589511826572E-4	0.021049518993432048	0.0012529475591328602	0.0
22	0.0012529475591328602	2.50589511826572E-4	0.02130010850525862	0.002004716094612576	0.0
23	0.0012529475591328602	2.50589511826572E-4	0.021550698017085194	0.0025058951182657203	0.0
24	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.0032576636537454363	0.0
25	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.0032576636537454363	0.0
26	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.003508253165572008	0.0
27	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.004510611212878296	0.0
28	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.012780065103155172	0.0
29	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.02355541411169777	0.0
30	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.04635905968791582	0.0
31	0.0012529475591328602	2.50589511826572E-4	0.021801287528911764	0.11176292227465112	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGACC	20	0.0018404968	37.0	3
GGTATCA	375	0.0	29.599998	1
ATACGGA	45	3.999641E-6	28.777779	4
GTATTAG	85	1.8189894E-12	28.294119	1
TAATACG	40	5.9305643E-5	27.75	2
CACGGTA	60	1.333754E-6	24.666668	2
ACGGTAC	60	1.333754E-6	24.666668	3
CGGACAG	40	0.0019289907	23.125	5
GTATCAA	1100	0.0	22.704544	1
AGATTGG	50	2.6978218E-4	22.199999	14
GCACCGC	45	0.0038214976	20.555557	10
TCGGACA	45	0.0038214976	20.555557	4
CCGTTAA	45	0.0038214976	20.555557	16
ACCATCG	110	1.7444108E-9	20.181818	18
CGGAGGG	55	5.135012E-4	20.181818	7
AGTGTTC	55	5.135012E-4	20.181818	8
TACGGAG	65	6.88771E-5	19.923077	5
TCACGGT	75	9.2409355E-6	19.733334	1
TCATATA	85	1.2411438E-6	19.588236	2
TCCGCCG	60	9.222417E-4	18.5	31
>>END_MODULE