FastQCFastQC Report
Fri 10 Feb 2017
ERR1633138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504660
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23270.4611025244719217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.34775888717156106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7780.15416319898545555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.029.1374991
GTATCAA11400.023.368421
CCGTCCG1005.329639E-1022.19999928
GCGGGTA855.1662937E-821.76470622
TGCGGGT957.1213435E-921.42105321
CGTCCGC1059.786163E-1021.14285929
CGGGTAA909.451833E-820.55555523
GGTAACG909.451833E-820.55555525
AACGTCA951.6714694E-719.47368428
CGAGTCG1153.0377123E-919.30434821
GAGTCGC1254.110916E-1019.2422
ATTAGAG1501.2732926E-1118.53
ATTGTAC701.21747864E-418.53
TTAACGG1103.8387952E-818.535
CCGCCGC1205.153197E-918.532
AGCGTCG1002.866982E-718.49999817
ACGTCAA1002.866982E-718.49999829
GCCGTCC1451.546141E-1017.86206827
CGTCAAT1054.7840877E-717.6190530
CGTCGGT953.598283E-617.52631619
Produced by FastQC (version 0.11.5)