FastQCFastQC Report
Fri 10 Feb 2017
ERR1633137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321919
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9440.2932414675741413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.2186885520891901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC303.5937745E-430.8333323
ACTATGA250.005490539629.68
GCCTAAT250.005490539629.635
AGTGTTA250.005490539629.68
GGTATCA2050.028.8780481
GTCATAA400.001928184323.1250021
GTATCAA5300.022.3396221
ATATAGC603.7155693E-521.5833326
GCCGTTA705.083115E-621.14285915
CCGTTAA705.083115E-621.14285916
TATTAGA1151.3460522E-1020.9130422
CGCGCGC450.003819917320.55555510
TATCGCG450.003819917320.5555557
TCGCGCG450.003819917320.5555559
CCGTACA555.1320455E-420.18181817
CGTTAAA759.231833E-619.73333417
GGTACCG801.610786E-518.56
GGCCGTT801.610786E-518.514
CAACCCA1205.133188E-918.517
ACGCTTC500.00702415318.525
Produced by FastQC (version 0.11.5)