FastQCFastQC Report
Fri 10 Feb 2017
ERR1633136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437840
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67711.5464553261465377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54971.2554814544125708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27340.6244290151653572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.33779462817467565No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13510.30856020464096473No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10620.242554357756258No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10610.2423259638224009No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9450.21583226749497536No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9110.2080668737438334No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8600.1964187831171204No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8310.189795359035264No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8160.18636945002740726No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8010.18294354101955054No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.17266581399598027No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7430.16969669285583777No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7370.16832632925269506No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7350.1678695413849808No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7320.16718435958340946No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7120.16261648090626712No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6910.15782020829526766No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6740.1539375114196967No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6690.1527955417504111No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6690.1527955417504111No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6160.14069066325598392No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6160.14069066325598392No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6090.1390919057189841No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6010.13726475424812717No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5970.1363511785126987No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5880.13429563310798465No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5830.13315366343869908No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5750.13132651196784215No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5690.12995614836469943No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5260.12013520920884341No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5190.1185364516718436No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5150.11762287593641513No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5100.11648090626712954No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5010.1144253608624155No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4900.11191302758998721No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4830.1103142700529874No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4820.11008587611913029No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4780.1091723003837018No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.1089439064498447No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4760.10871551251598757No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4660.1064315731774164No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4650.1062031792435593No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4590.10483281564041659No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4590.10483281564041659No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4560.10414763383884525No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4550.10391923990498812No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4520.10323405810341676No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4500.10277727023570253No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4500.10277727023570253No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4490.10254887630184542No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4480.1023204823679883No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4420.10095011876484561No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.1007217248309885No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4380.10003654302941714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACT401.5970727E-632.3754
CACTGTG401.5970727E-632.3757
AAGGTGT352.3849563E-531.71428514
CGGGTTA250.005492672329.617
GCGACAG250.005492672329.635
CGACAGC250.005492672329.636
TACGAGT250.005492672329.611
GAACCTA250.005492672329.66
GGTATCA8800.029.0113641
ACTGTGC454.000809E-628.7777778
ATTAGAG1300.027.0384623
GACAGCG358.86059E-426.4285737
GATCAAC358.86059E-426.428571
AGGCACC1350.026.0370378
CTAGTGC509.074311E-625.9000029
CCCTTGC751.3687895E-824.66666632
AGTGACA1450.024.24137923
GTGACAC1450.024.24137924
GTATCAA25850.023.8317221
TCCCTTG701.9172694E-723.78571331
Produced by FastQC (version 0.11.5)