FastQCFastQC Report
Fri 10 Feb 2017
ERR1633135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435877
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17260.3959832705097998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.28586046063453685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6360.14591272308472344No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6160.14132427267325415No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5720.13122968176802172No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5570.12778834395941974No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4940.11333472516329149No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4860.11149934499870376No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4850.11126992247813029No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4830.11081107743698337No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4560.10461666938149984No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4540.1041578243403529No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4480.1027812892169121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAA358.860525E-426.4285721
GGTATCA2850.025.3157881
GTATCAA9050.024.3259661
TTAACGG851.9681465E-923.94117735
AACGGCC903.812602E-922.61111337
AACTGTA450.003822054720.5555577
TATTAGA1700.019.5882362
TATACAG851.2416822E-619.5882365
TAACGGC1052.2497261E-819.3809536
ATGCTAA1306.9303496E-1018.55
CGGTACT609.2242763E-418.54
CCTATAT609.2242763E-418.51
CTGTCTA500.0070280518.4999989
ATTAGAC500.0070280518.4999983
GGACTGA500.0070280518.4999986
GGACCCC500.0070280518.4999986
GGGGCTA500.0070280518.49999818
ACGGTAC500.0070280518.4999983
AGCACGT500.0070280518.49999835
ATTAGAG1451.546141E-1017.862073
Produced by FastQC (version 0.11.5)