##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633135.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435877
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.715880856296614	33.0	33.0	33.0	27.0	33.0
2	31.25348435453121	33.0	33.0	33.0	27.0	33.0
3	31.691119283651123	33.0	33.0	33.0	27.0	33.0
4	31.833703085962323	33.0	33.0	33.0	27.0	33.0
5	31.945101485051975	33.0	33.0	33.0	33.0	33.0
6	34.83007362168685	37.0	37.0	37.0	27.0	37.0
7	34.992279932182704	37.0	37.0	37.0	33.0	37.0
8	35.16616155475054	37.0	37.0	37.0	33.0	37.0
9	35.24106800771777	37.0	37.0	37.0	33.0	37.0
10	35.282015339189726	37.0	37.0	37.0	33.0	37.0
11	35.29723293497936	37.0	37.0	37.0	33.0	37.0
12	35.27429527137243	37.0	37.0	37.0	33.0	37.0
13	35.28099440897317	37.0	37.0	37.0	33.0	37.0
14	35.26018578635716	37.0	37.0	37.0	33.0	37.0
15	35.27249659881113	37.0	37.0	37.0	33.0	37.0
16	35.27310915694106	37.0	37.0	37.0	33.0	37.0
17	35.24564498700322	37.0	37.0	37.0	33.0	37.0
18	35.26252589606701	37.0	37.0	37.0	33.0	37.0
19	35.25590705634846	37.0	37.0	37.0	33.0	37.0
20	35.2538422536633	37.0	37.0	37.0	33.0	37.0
21	35.2464800849781	37.0	37.0	37.0	33.0	37.0
22	35.247209648593525	37.0	37.0	37.0	33.0	37.0
23	35.24555321799498	37.0	37.0	37.0	33.0	37.0
24	35.23522461611877	37.0	37.0	37.0	33.0	37.0
25	35.22963817774281	37.0	37.0	37.0	33.0	37.0
26	35.1898677838014	37.0	37.0	37.0	33.0	37.0
27	35.11672329579216	37.0	37.0	37.0	33.0	37.0
28	35.11292176462626	37.0	37.0	37.0	33.0	37.0
29	35.15904716238755	37.0	37.0	37.0	33.0	37.0
30	35.114649316206176	37.0	37.0	37.0	33.0	37.0
31	35.10796394395667	37.0	37.0	37.0	33.0	37.0
32	35.15168269947715	37.0	37.0	37.0	33.0	37.0
33	35.12839631363894	37.0	37.0	37.0	33.0	37.0
34	35.062421279397626	37.0	37.0	37.0	33.0	37.0
35	35.06134528777614	37.0	37.0	37.0	27.0	37.0
36	35.04013517574912	37.0	37.0	37.0	27.0	37.0
37	35.061785779015636	37.0	37.0	37.0	27.0	37.0
38	34.91190863477541	37.0	37.0	37.0	27.0	37.0
39	34.98720969447803	37.0	37.0	37.0	27.0	37.0
40	35.01462568568656	37.0	37.0	37.0	27.0	37.0
41	35.03245410976032	37.0	37.0	37.0	27.0	37.0
42	34.94579663528931	37.0	37.0	37.0	27.0	37.0
43	34.72708814642663	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	14.0
16	19.0
17	19.0
18	19.0
19	19.0
20	38.0
21	76.0
22	262.0
23	600.0
24	1204.0
25	2077.0
26	3499.0
27	5523.0
28	7463.0
29	10195.0
30	13290.0
31	17093.0
32	21523.0
33	28366.0
34	40474.0
35	72767.0
36	211337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32421990607442	21.242919447458803	14.148486843765557	24.28437380270122
2	15.635144777081608	23.465335404253953	38.63589957717429	22.263620241490145
3	17.644886057305158	27.13724284603225	31.34554014091131	23.872330955751277
4	12.693948063329794	18.437540865886476	39.33999729281425	29.528513777969472
5	13.431082621932333	37.535359745983385	35.64101799360829	13.392539638475991
6	28.6317011450478	40.211802870993424	17.854119396068157	13.302376587890622
7	25.537250187552914	33.11163470428584	23.72550054258426	17.625614565576985
8	23.72733592274885	36.491028432332975	21.154591777038018	18.627043867880158
9	25.504901612152054	16.003138500081445	20.517256014884932	37.97470387288157
10	14.692447640045241	28.54291462958587	34.172484439417545	22.592153290951348
11	32.86936452256026	24.321081405992974	24.713623338694173	18.09593073275259
12	22.263620241490145	26.287920674869287	30.363382330336307	21.085076753304257
13	27.23589452987884	22.660980047123385	27.164544125980495	22.938581297017276
14	21.590953411168748	21.890349800517118	28.1051764603317	28.413520327982432
15	23.556874989962765	29.020801739940396	25.347976608079804	22.074346662017035
16	22.562787208317943	29.374571266664677	25.233265347793072	22.829376177224308
17	22.106465814897323	28.14807847167894	26.455399114887918	23.290056598535823
18	22.05209267752141	27.808303718709638	28.335975516028604	21.80362808774035
19	24.34723557333835	26.96494653308158	28.118253544004386	20.569564349575682
20	24.09831213851614	26.34114669964233	28.47454671845498	21.085994443386554
21	22.198923090688428	28.00193632607364	27.4359509678189	22.363189615419028
22	21.956423486442276	27.89777850173329	27.416450053570156	22.72934795825428
23	22.068381676482126	27.788114536899172	28.1535846121727	21.989919174446
24	23.098488793856983	27.956740089520665	26.645590384443317	22.299180732179032
25	23.13611408723103	27.23566510735827	27.806697761065625	21.821523044345078
26	22.71948278986962	27.55043280558506	27.57842235309502	22.151662051450295
27	22.60821286739149	27.407731997788364	27.310686271585794	22.67336886323435
28	21.735030754088882	27.71561702039796	28.15289634461098	22.39645588090218
29	23.173509958084505	27.60641190060499	27.246677388345798	21.97340075296471
30	22.445322877784328	27.57016314235438	27.68005652970907	22.30445745015222
31	22.911509439589608	27.272602133170597	27.646790264225917	22.169098163013878
32	21.505378810994845	27.37951302775783	28.06479809671077	23.050310064536557
33	21.820605354262785	27.268472527800274	28.44219814305412	22.468723974882824
34	22.02089121472342	27.34418195958952	28.03313778887163	22.601789036815433
35	22.458170538936443	28.108388375619725	27.2760434709792	22.15739761446463
36	22.82593483941571	27.660555615460325	27.279255386267227	22.234254158856743
37	23.19438740745669	26.656832087951415	27.42493868683138	22.723841817760515
38	23.009243433353905	27.27283155569117	27.338446396575183	22.379478614379742
39	22.35080079930806	27.197580968943075	27.99069462256554	22.460923609183325
40	22.378102079256305	27.683039022476525	27.79385009991351	22.145008798353665
41	22.1092188851442	27.073922230353975	28.14693135907607	22.669927525425752
42	22.4127448798629	27.236812219961138	27.771825537938454	22.578617362237512
43	22.386361289996948	26.639854821428983	27.98679443971579	22.986989448858278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	22.5
2	34.0
3	106.0
4	178.0
5	178.0
6	234.5
7	291.0
8	269.5
9	248.0
10	354.5
11	461.0
12	461.0
13	704.0
14	947.0
15	1848.0
16	2749.0
17	3119.5
18	3490.0
19	3490.0
20	3458.0
21	3426.0
22	4093.5
23	4761.0
24	5872.5
25	6984.0
26	6984.0
27	8369.0
28	9754.0
29	12556.0
30	15358.0
31	17191.0
32	19024.0
33	19024.0
34	21159.5
35	23295.0
36	24974.5
37	26654.0
38	29264.5
39	31875.0
40	31875.0
41	33851.5
42	35828.0
43	34830.0
44	33832.0
45	33692.0
46	33552.0
47	33552.0
48	33027.0
49	32502.0
50	32863.5
51	33225.0
52	33068.5
53	32912.0
54	32912.0
55	28731.5
56	24551.0
57	21499.5
58	18448.0
59	15997.5
60	13547.0
61	13547.0
62	11778.0
63	10009.0
64	8209.5
65	6410.0
66	5283.0
67	4156.0
68	4156.0
69	3507.5
70	2859.0
71	2310.5
72	1762.0
73	1489.0
74	1216.0
75	1216.0
76	927.0
77	638.0
78	505.0
79	372.0
80	294.0
81	216.0
82	216.0
83	172.5
84	129.0
85	109.0
86	89.0
87	68.0
88	47.0
89	47.0
90	36.5
91	26.0
92	18.5
93	11.0
94	5.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	435877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.06548482973575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.74973887660968	47.44232176046614
2	6.356422002039302	6.87326074645309
3	1.7825085100165	2.8911656042151583
4	0.8849196010539042	1.9137442906526267
5	0.5280726250712897	1.4275251249895275
6	0.3602166724116597	1.168517342261429
7	0.2707222903480074	1.024571231731706
8	0.2119620198081347	0.9167863493133486
9	0.17462805734452727	0.8497215526686088
>10	1.3523236397786287	15.05732556099319
>50	0.20626843910500195	7.782521626797504
>100	0.11964427133097848	11.411443737863728
>500	0.0017153300549244228	0.552035749199568
>1k	8.576650274622114E-4	0.6890593223944208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1726	0.3959832705097998	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1246	0.28586046063453685	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	636	0.14591272308472344	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	616	0.14132427267325415	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	572	0.13122968176802172	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	557	0.12778834395941974	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	494	0.11333472516329149	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	486	0.11149934499870376	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	485	0.11126992247813029	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	483	0.11081107743698337	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	456	0.10461666938149984	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	454	0.1041578243403529	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	448	0.1027812892169121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.2942252057346453E-4	0.0	0.0
4	0.0	0.0	2.2942252057346453E-4	0.0	0.0
5	0.0	0.0	6.882675617203936E-4	2.2942252057346453E-4	0.0
6	0.0	0.0	0.0011471126028673227	2.2942252057346453E-4	0.0
7	0.0	0.0	0.002982492767455039	2.2942252057346453E-4	0.0
8	0.0	0.0	0.0032119152880285035	2.2942252057346453E-4	0.0
9	0.0	0.0	0.003900182849748897	2.2942252057346453E-4	0.0
10	4.5884504114692906E-4	0.0	0.004129605370322362	2.2942252057346453E-4	0.0
11	4.5884504114692906E-4	0.0	0.00504729545261622	2.2942252057346453E-4	0.0
12	4.5884504114692906E-4	0.0	0.00504729545261622	2.2942252057346453E-4	0.0
13	4.5884504114692906E-4	0.0	0.005506140493763149	2.2942252057346453E-4	0.0
14	4.5884504114692906E-4	0.0	0.005964985534910078	2.2942252057346453E-4	0.0
15	4.5884504114692906E-4	0.0	0.0061944080554835424	2.2942252057346453E-4	0.0
16	4.5884504114692906E-4	0.0	0.0061944080554835424	2.2942252057346453E-4	0.0
17	4.5884504114692906E-4	0.0	0.0061944080554835424	2.2942252057346453E-4	0.0
18	4.5884504114692906E-4	0.0	0.0061944080554835424	4.5884504114692906E-4	0.0
19	4.5884504114692906E-4	0.0	0.0061944080554835424	4.5884504114692906E-4	0.0
20	4.5884504114692906E-4	0.0	0.006423830576057007	4.5884504114692906E-4	0.0
21	4.5884504114692906E-4	0.0	0.0066532530966304715	4.5884504114692906E-4	0.0
22	4.5884504114692906E-4	0.0	0.006882675617203936	6.882675617203936E-4	0.0
23	4.5884504114692906E-4	0.0	0.006882675617203936	0.0011471126028673227	0.0
24	4.5884504114692906E-4	0.0	0.006882675617203936	0.0013765351234407872	0.0
25	4.5884504114692906E-4	0.0	0.006882675617203936	0.0013765351234407872	0.0
26	4.5884504114692906E-4	0.0	0.006882675617203936	0.0016059576440142517	0.0
27	4.5884504114692906E-4	0.0	0.0071120981377774005	0.0018353801645877162	0.0
28	4.5884504114692906E-4	0.0	0.007341520658350865	0.010324013425805904	0.0
29	4.5884504114692906E-4	0.0	0.007341520658350865	0.025007054742507633	0.0
30	4.5884504114692906E-4	0.0	0.007341520658350865	0.048408151841001015	0.0
31	4.5884504114692906E-4	0.0	0.007341520658350865	0.10553435946379368	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTAA	35	8.860525E-4	26.428572	1
GGTATCA	285	0.0	25.315788	1
GTATCAA	905	0.0	24.325966	1
TTAACGG	85	1.9681465E-9	23.941177	35
AACGGCC	90	3.812602E-9	22.611113	37
AACTGTA	45	0.0038220547	20.555557	7
TATTAGA	170	0.0	19.588236	2
TATACAG	85	1.2416822E-6	19.588236	5
TAACGGC	105	2.2497261E-8	19.38095	36
ATGCTAA	130	6.9303496E-10	18.5	5
CGGTACT	60	9.2242763E-4	18.5	4
CCTATAT	60	9.2242763E-4	18.5	1
CTGTCTA	50	0.00702805	18.499998	9
ATTAGAC	50	0.00702805	18.499998	3
GGACTGA	50	0.00702805	18.499998	6
GGACCCC	50	0.00702805	18.499998	6
GGGGCTA	50	0.00702805	18.499998	18
ACGGTAC	50	0.00702805	18.499998	3
AGCACGT	50	0.00702805	18.499998	35
ATTAGAG	145	1.546141E-10	17.86207	3
>>END_MODULE