FastQCFastQC Report
Fri 10 Feb 2017
ERR1633134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406901
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10770.2646835471035952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.19095553955384725No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5440.13369345369020966No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4640.11403265167694353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4200.10321921056964717No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4120.10125313036832054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT250.005492224429.59999825
TAGCGTT250.005492224429.59999826
GGACCGT250.005492224429.5999986
GTTATGC250.005492224429.5999983
GTATCAA6950.023.6906471
TCGGCGT400.00192905523.12500211
TAAGGCA400.00192905523.1250024
ATTATAC957.1104296E-921.4210533
TGGGGCG705.0885665E-621.14285937
GTACTAA450.003821625620.5555551
GGTATCA2250.019.7333341
GTATTAT951.6694685E-719.4736841
GTGCACT609.2228444E-418.54
CGGTTGG801.6124914E-518.533
GCAGTAC701.2167425E-418.53
TTACCCC609.2228444E-418.54
CAATACT500.00702726718.4999984
ATTAGAG1351.1441443E-917.8148163
AACCGTG1054.778467E-717.6190478
TAGACTG852.7161223E-517.4117645
Produced by FastQC (version 0.11.5)