FastQCFastQC Report
Fri 10 Feb 2017
ERR1633133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406058
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11980.29503174423358236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.20711326953292386No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC8090.19923262194070798No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7940.19553856838185676No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7680.18913554221318135No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6260.15416516852272336No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT6250.15391889828546662No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6200.15268754709918286No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5820.14332927808342652No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5560.1369262519147511No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA5360.13200084716961616No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5080.12510528052642728No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA5040.12412019957740027No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4990.12288884839111655No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4740.11673209245969787No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4730.11648582222244114No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4530.1115604174773062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4110.10121706751252285No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC4100.10097079727526609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6400.028.0390641
GGTATCA1650.028.0303021
GGTCGGT400.001929048123.12500211
CTGACGC502.697933E-422.227
TAGGGGT502.697933E-422.24
CGTGCAA656.8880894E-519.92307711
TAACCGT656.8880894E-519.9230777
GGGGTTA656.8880894E-519.9230776
GGGTTAG656.8880894E-519.9230777
ATCAACG9250.019.03
GTCGCCA902.1452488E-618.512
CGAACTA500.007027237718.524
ATAATAC609.2227996E-418.53
CGCAGGC609.2227996E-418.531
TCAACGC9500.018.54
AGGCTTA609.2227996E-418.534
TCTATGC500.007027237718.53
CAACGCA9850.017.842645
TATCAAC9950.017.6633172
TCGCCAT1054.778394E-717.61904713
Produced by FastQC (version 0.11.5)