FastQCFastQC Report
Fri 10 Feb 2017
ERR1633132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335470
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9600.2861656780039944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.20985483053626253No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3970.1183414314245685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3940.11744716368080603No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3850.1147643604495186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1600.024.2812521
TATGACC400.001928352423.1250023
GTAAGAT400.001928352423.1250023
GTATCAA4700.022.436171
TAGAGCA500.00702475518.54
GAACTCG500.00702475518.529
ATACTCT500.00702475518.56
TATCGTG500.00702475518.537
CATGCTA1351.1423253E-917.8148164
GTACATG1553.965397E-1016.7096771
TAGATTG1005.8528694E-616.65000213
CGAACTA1251.6473496E-716.27999929
TGATAGA1151.2357159E-616.08695610
CATACTG700.002587365515.8571435
GTCCAAT1059.303631E-615.85714220
GCGAACT1302.572815E-715.65384628
ACTGTGC957.035881E-515.5789488
CCTATAC1201.9254803E-615.4166663
TTTAAGA855.350103E-415.2352932
ATCAACG6950.015.1726633
Produced by FastQC (version 0.11.5)