##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633132.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335470
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.662676245267832	33.0	33.0	33.0	27.0	33.0
2	31.212713506423825	33.0	33.0	33.0	27.0	33.0
3	31.644588189704	33.0	33.0	33.0	27.0	33.0
4	31.77398575133395	33.0	33.0	33.0	27.0	33.0
5	31.8742510507646	33.0	33.0	33.0	33.0	33.0
6	34.76744269234209	37.0	37.0	37.0	27.0	37.0
7	34.91589709959162	37.0	37.0	37.0	27.0	37.0
8	35.11184010492742	37.0	37.0	37.0	33.0	37.0
9	35.17473991713119	37.0	37.0	37.0	33.0	37.0
10	35.22300950904701	37.0	37.0	37.0	33.0	37.0
11	35.2351536650073	37.0	37.0	37.0	33.0	37.0
12	35.17767907711569	37.0	37.0	37.0	33.0	37.0
13	35.210239365666084	37.0	37.0	37.0	33.0	37.0
14	35.18422809789251	37.0	37.0	37.0	33.0	37.0
15	35.19124809968105	37.0	37.0	37.0	33.0	37.0
16	35.18655617491877	37.0	37.0	37.0	33.0	37.0
17	35.15102989835157	37.0	37.0	37.0	33.0	37.0
18	35.17462068143202	37.0	37.0	37.0	33.0	37.0
19	35.168617163978894	37.0	37.0	37.0	33.0	37.0
20	35.16991981399231	37.0	37.0	37.0	33.0	37.0
21	35.16949354636778	37.0	37.0	37.0	33.0	37.0
22	35.16219930247116	37.0	37.0	37.0	33.0	37.0
23	35.184174441827885	37.0	37.0	37.0	33.0	37.0
24	35.16315616895699	37.0	37.0	37.0	33.0	37.0
25	35.15777267713953	37.0	37.0	37.0	33.0	37.0
26	35.09974364324679	37.0	37.0	37.0	33.0	37.0
27	35.03054818612693	37.0	37.0	37.0	27.0	37.0
28	35.02534652875071	37.0	37.0	37.0	27.0	37.0
29	35.05350702000179	37.0	37.0	37.0	27.0	37.0
30	35.011470474259994	37.0	37.0	37.0	27.0	37.0
31	34.99975258592423	37.0	37.0	37.0	27.0	37.0
32	35.04055206128715	37.0	37.0	37.0	27.0	37.0
33	35.010296002623186	37.0	37.0	37.0	27.0	37.0
34	34.95908128893791	37.0	37.0	37.0	27.0	37.0
35	34.95083614034042	37.0	37.0	37.0	27.0	37.0
36	34.92338510149939	37.0	37.0	37.0	27.0	37.0
37	34.95054401287746	37.0	37.0	37.0	27.0	37.0
38	34.7997704712791	37.0	37.0	37.0	27.0	37.0
39	34.876942796673326	37.0	37.0	37.0	27.0	37.0
40	34.90408978448147	37.0	37.0	37.0	27.0	37.0
41	34.934846633081946	37.0	37.0	37.0	27.0	37.0
42	34.84277580707664	37.0	37.0	37.0	27.0	37.0
43	34.619712641965	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	17.0
17	22.0
18	6.0
19	12.0
20	29.0
21	78.0
22	201.0
23	507.0
24	1063.0
25	1888.0
26	3054.0
27	4526.0
28	6312.0
29	8186.0
30	10791.0
31	13434.0
32	17190.0
33	22290.0
34	31438.0
35	56900.0
36	157519.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.98181655587683	21.222464005723314	13.824783140072139	24.97093629832772
2	15.812442245208217	22.912331952186484	38.11488359614869	23.160342206456612
3	17.321370018183444	27.266819685813932	30.236384773601216	25.175425522401408
4	12.508421021253763	18.2281575103586	39.648552776701344	29.61486869168629
5	13.526991981399231	37.44418278832683	35.94837094226011	13.080454288013833
6	29.119742450889795	40.51301159567174	17.595612126270606	12.771633827167856
7	26.470325215369485	32.21212030882047	24.144632903091185	17.17292157271887
8	24.08113989328405	36.5049035681283	20.75774286821474	18.65621367037291
9	25.455927504694902	15.924225713178524	20.462336423525205	38.157510358601364
10	14.628133663218767	28.21325304796256	34.56434256416371	22.59427072465496
11	33.567532119116464	23.988136047932752	24.581333651295196	17.862998181655588
12	22.443139475959104	26.14183086416073	30.569052374280858	20.84597728559931
13	27.355054103198494	22.38083882314365	26.436343041106507	23.827764032551347
14	21.673771127075447	21.136614302322116	27.88952812472054	29.300086445881895
15	23.433988136047933	29.238381971562287	24.467463558589444	22.86016633380034
16	22.97642114048946	28.79989268787075	25.178108325632692	23.045577846007095
17	22.146242585029956	28.148865770411664	26.77497242674457	22.92991921781381
18	22.438071958744448	28.113095060661163	27.80844784928608	21.640385131308314
19	24.13181506543059	27.050704981071334	27.447461770053955	21.370018183444124
20	23.55203147822458	27.065609443467377	27.730348466330817	21.652010611977225
21	22.71589113780666	27.4367305571288	27.59054460905595	22.256833696008584
22	22.253256625033536	27.394699973171967	27.65433570811101	22.69770769368349
23	22.374578948937312	27.218529227650755	28.13485557575938	22.272036247652547
24	22.675649089337348	27.917548514025096	26.490595284228096	22.916207112409456
25	22.853906459594004	27.230750886815514	27.746147196470623	22.169195457119862
26	22.627954809670015	27.67609622320923	27.645094941425462	22.050854025695294
27	22.42376367484425	27.71961725340567	26.962172474438844	22.894446597311237
28	22.382031180135336	27.273377649268195	28.153933287626316	22.19065788297016
29	23.767251915223415	27.061734283244405	27.037290964914895	22.133722836617284
30	22.963603302828865	26.929084567919638	27.713357379199334	22.393954750052167
31	22.8738784392047	27.11509225862223	27.582794288610007	22.42823501356306
32	22.156675708707187	26.95531642173667	27.747041464214384	23.14096640534176
33	22.043997972993115	27.135064238232925	28.11041225742987	22.710525531344082
34	22.82439562404984	26.807464154767935	27.7300503770829	22.638089844099323
35	22.684889856022895	27.583688556353774	27.425403165707813	22.30601842191552
36	23.509404715771904	27.234327957790562	26.8512832742123	22.404984052225235
37	22.606492383819717	26.83757116880794	27.351775121471366	23.204161325900973
38	22.73824783140072	27.402450293617907	27.250126687930365	22.609175187051004
39	22.184994187259665	27.308254091274925	27.723194324380717	22.783557397084685
40	22.425850299579693	27.524666885265447	27.944972724833818	22.104510090321043
41	22.406772587712762	26.73413419977941	27.764628729841718	23.094464482666112
42	22.24192923361254	27.587861805824666	27.57206307568486	22.598145884877933
43	22.91739946940114	26.489104837988492	27.56908218320565	23.024413509404713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	16.0
2	23.0
3	61.5
4	100.0
5	100.0
6	127.0
7	154.0
8	155.0
9	156.0
10	198.0
11	240.0
12	240.0
13	378.0
14	516.0
15	949.0
16	1382.0
17	1648.5
18	1915.0
19	1915.0
20	1989.5
21	2064.0
22	2415.5
23	2767.0
24	3664.5
25	4562.0
26	4562.0
27	5917.0
28	7272.0
29	8941.0
30	10610.0
31	12604.0
32	14598.0
33	14598.0
34	16851.0
35	19104.0
36	20932.0
37	22760.0
38	24529.5
39	26299.0
40	26299.0
41	27313.5
42	28328.0
43	28480.0
44	28632.0
45	27744.5
46	26857.0
47	26857.0
48	25724.5
49	24592.0
50	23886.0
51	23180.0
52	21746.5
53	20313.0
54	20313.0
55	19556.5
56	18800.0
57	16180.5
58	13561.0
59	12586.0
60	11611.0
61	11611.0
62	10090.0
63	8569.0
64	7227.5
65	5886.0
66	4907.0
67	3928.0
68	3928.0
69	3208.5
70	2489.0
71	2111.5
72	1734.0
73	1377.5
74	1021.0
75	1021.0
76	805.0
77	589.0
78	469.5
79	350.0
80	278.0
81	206.0
82	206.0
83	162.0
84	118.0
85	111.5
86	105.0
87	77.5
88	50.0
89	50.0
90	32.5
91	15.0
92	10.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	335470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.96680153663065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.29408041782709	59.797389617365646
2	5.921114247928778	7.930361654335251
3	1.5850034107286182	3.184278265234382
4	0.7741302521673576	2.073641078415731
5	0.4745694136666351	1.5890197870184368
6	0.33420184736475805	1.3428257271390651
7	0.23818438433758243	1.116531247654161
8	0.18294461466072928	0.9800972561744343
9	0.15244008279063645	0.9187582293421287
>10	0.9177602896492334	12.068468965218878
>50	0.08610585523103548	3.9225405518750556
>100	0.03856824765556096	4.576347364632392
>500	8.969359919897896E-4	0.49974025559445473
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	960	0.2861656780039944	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	704	0.20985483053626253	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	397	0.1183414314245685	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	394	0.11744716368080603	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	385	0.1147643604495186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.980892479208275E-4	0.0	0.0
2	0.0	0.0	2.980892479208275E-4	0.0	0.0
3	0.0	0.0	2.980892479208275E-4	0.0	0.0
4	0.0	0.0	2.980892479208275E-4	0.0	0.0
5	0.0	0.0	2.980892479208275E-4	0.0	0.0
6	0.0	0.0	5.96178495841655E-4	0.0	0.0
7	0.0	0.0	0.00119235699168331	0.0	0.0
8	0.0	0.0	0.0020866247354457924	0.0	0.0
9	0.0	0.0	0.0020866247354457924	0.0	0.0
10	0.0	0.0	0.0026828032312874476	0.0	0.0
11	0.0	0.0	0.00357707097504993	0.0	0.0
12	0.0	0.0	0.00357707097504993	0.0	0.0
13	2.980892479208275E-4	0.0	0.004173249470891585	0.0	0.0
14	2.980892479208275E-4	0.0	0.004471338718812412	2.980892479208275E-4	0.0
15	2.980892479208275E-4	0.0	0.00476942796673324	2.980892479208275E-4	0.0
16	2.980892479208275E-4	0.0	0.00476942796673324	2.980892479208275E-4	0.0
17	2.980892479208275E-4	0.0	0.00476942796673324	2.980892479208275E-4	0.0
18	2.980892479208275E-4	0.0	0.005365606462574895	2.980892479208275E-4	0.0
19	2.980892479208275E-4	0.0	0.005365606462574895	5.96178495841655E-4	0.0
20	2.980892479208275E-4	0.0	0.005365606462574895	8.942677437624825E-4	0.0
21	2.980892479208275E-4	0.0	0.005365606462574895	0.0014904462396041374	0.0
22	2.980892479208275E-4	0.0	0.005663695710495722	0.001788535487524965	0.0
23	2.980892479208275E-4	0.0	0.005663695710495722	0.001788535487524965	0.0
24	2.980892479208275E-4	0.0	0.005663695710495722	0.0026828032312874476	0.0
25	2.980892479208275E-4	0.0	0.005663695710495722	0.002980892479208275	0.0
26	2.980892479208275E-4	0.0	0.00596178495841655	0.002980892479208275	0.0
27	2.980892479208275E-4	0.0	0.00596178495841655	0.004173249470891585	0.0
28	2.980892479208275E-4	0.0	0.00596178495841655	0.012817837660595582	0.0
29	2.980892479208275E-4	0.0	0.00596178495841655	0.020866247354457926	0.0
30	2.980892479208275E-4	0.0	0.00596178495841655	0.036366888246340955	0.0
31	2.980892479208275E-4	0.0	0.006259874206337377	0.07303186574060273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	160	0.0	24.281252	1
TATGACC	40	0.0019283524	23.125002	3
GTAAGAT	40	0.0019283524	23.125002	3
GTATCAA	470	0.0	22.43617	1
TAGAGCA	50	0.007024755	18.5	4
GAACTCG	50	0.007024755	18.5	29
ATACTCT	50	0.007024755	18.5	6
TATCGTG	50	0.007024755	18.5	37
CATGCTA	135	1.1423253E-9	17.814816	4
GTACATG	155	3.965397E-10	16.709677	1
TAGATTG	100	5.8528694E-6	16.650002	13
CGAACTA	125	1.6473496E-7	16.279999	29
TGATAGA	115	1.2357159E-6	16.086956	10
CATACTG	70	0.0025873655	15.857143	5
GTCCAAT	105	9.303631E-6	15.857142	20
GCGAACT	130	2.572815E-7	15.653846	28
ACTGTGC	95	7.035881E-5	15.578948	8
CCTATAC	120	1.9254803E-6	15.416666	3
TTTAAGA	85	5.350103E-4	15.235293	2
ATCAACG	695	0.0	15.172663	3
>>END_MODULE