FastQCFastQC Report
Fri 10 Feb 2017
ERR1633131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350325
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14320.4087632912295725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.2925854563619496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5550.15842432027403125No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4400.1255976593163491No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3980.11360879183615213No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3770.10761435809605367No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3700.10561621351602084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGC250.005491191529.63
GTATCAA6800.024.4852941
TGCGGGT400.001928522423.12521
TATACCC400.001928522423.1254
GGTATCA2300.021.7173921
GAGTTAG450.003820580220.5555551
CCGGTGC450.003820580220.5555558
GTATAGA759.235648E-619.7333341
GTTAGGT1153.0286174E-919.3043481
TCTGCGG500.007025358318.519
CGGGTAA500.007025358318.523
AGCCGGT500.007025358318.56
TACCCTC1306.91216E-1018.55
TCACCTG609.2193513E-418.537
GGTAACG500.007025358318.525
TATATAG609.2193513E-418.52
ACGGGCG500.007025358318.516
GCCGCTC500.007025358318.527
ACTGTCA1700.018.512
GTATATA500.007025358318.51
Produced by FastQC (version 0.11.5)