##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633131.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350325
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654811960322558	33.0	33.0	33.0	27.0	33.0
2	31.19141654178263	33.0	33.0	33.0	27.0	33.0
3	31.632130164846927	33.0	33.0	33.0	27.0	33.0
4	31.769328480696498	33.0	33.0	33.0	27.0	33.0
5	31.87836723042889	33.0	33.0	33.0	33.0	33.0
6	34.752509812317136	37.0	37.0	37.0	27.0	37.0
7	34.935108827517304	37.0	37.0	37.0	27.0	37.0
8	35.10268750446014	37.0	37.0	37.0	33.0	37.0
9	35.17852565474916	37.0	37.0	37.0	33.0	37.0
10	35.20598016127881	37.0	37.0	37.0	33.0	37.0
11	35.23398273032184	37.0	37.0	37.0	33.0	37.0
12	35.20052522657532	37.0	37.0	37.0	33.0	37.0
13	35.240642260757866	37.0	37.0	37.0	33.0	37.0
14	35.19659744522943	37.0	37.0	37.0	33.0	37.0
15	35.190879897238275	37.0	37.0	37.0	33.0	37.0
16	35.19845857418112	37.0	37.0	37.0	33.0	37.0
17	35.17553985584814	37.0	37.0	37.0	33.0	37.0
18	35.1748091058303	37.0	37.0	37.0	33.0	37.0
19	35.18635838150289	37.0	37.0	37.0	33.0	37.0
20	35.18092628273746	37.0	37.0	37.0	33.0	37.0
21	35.1964861200314	37.0	37.0	37.0	33.0	37.0
22	35.17941054734889	37.0	37.0	37.0	33.0	37.0
23	35.17860558053236	37.0	37.0	37.0	33.0	37.0
24	35.16116748733319	37.0	37.0	37.0	33.0	37.0
25	35.1563062870192	37.0	37.0	37.0	33.0	37.0
26	35.12250338970956	37.0	37.0	37.0	33.0	37.0
27	35.08240919146507	37.0	37.0	37.0	33.0	37.0
28	35.04406194248198	37.0	37.0	37.0	27.0	37.0
29	35.076329122957254	37.0	37.0	37.0	33.0	37.0
30	35.01660458146007	37.0	37.0	37.0	27.0	37.0
31	35.01731820452437	37.0	37.0	37.0	27.0	37.0
32	35.03374295297224	37.0	37.0	37.0	27.0	37.0
33	35.01847427388853	37.0	37.0	37.0	27.0	37.0
34	34.9564861200314	37.0	37.0	37.0	27.0	37.0
35	34.944933989866556	37.0	37.0	37.0	27.0	37.0
36	34.9110254763434	37.0	37.0	37.0	27.0	37.0
37	34.94310996931421	37.0	37.0	37.0	27.0	37.0
38	34.797099835866696	37.0	37.0	37.0	27.0	37.0
39	34.891623492471275	37.0	37.0	37.0	27.0	37.0
40	34.91268679083708	37.0	37.0	37.0	27.0	37.0
41	34.924158995218725	37.0	37.0	37.0	27.0	37.0
42	34.81343609505459	37.0	37.0	37.0	27.0	37.0
43	34.61120102761721	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	19.0
17	18.0
18	8.0
19	17.0
20	37.0
21	86.0
22	209.0
23	502.0
24	1033.0
25	1901.0
26	3125.0
27	4674.0
28	6424.0
29	8747.0
30	11143.0
31	14154.0
32	17921.0
33	23464.0
34	33144.0
35	59464.0
36	164225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59430528794691	20.954542210804252	13.709555412830943	24.741597088417898
2	15.885534860486691	23.02861628487833	37.91936059373439	23.166488260900593
3	17.498037536573182	27.17933347605795	30.194533647327482	25.12809534004139
4	12.619995718261615	18.27788482123742	39.41711268108185	29.68500677941911
5	13.891672018839648	37.1312352815243	35.366302718903874	13.610789980732177
6	29.218011846142865	39.971455077428104	17.544565760365373	13.265967316063657
7	26.456005138086063	32.32055948048241	23.742239349175765	17.481196032255763
8	24.25604795546992	35.786198529936485	20.77670734318133	19.181046171412262
9	25.922215085991578	15.55441375865268	20.385071005494897	38.13830014986085
10	15.12852351387997	27.981731249553988	33.965032469849426	22.92471276671662
11	33.75037465210875	23.908656247769926	24.173267680011417	18.167701420109896
12	23.044315992292873	26.01926782273603	30.001284521515736	20.935131663455365
13	27.711696282023834	22.461714122600444	26.33982730321844	23.48676229215728
14	21.977306786555342	20.956254906158566	27.819596089345605	29.246842217940483
15	23.944051951759082	28.59002354956112	24.705345036751588	22.76057946192821
16	22.957539427674302	28.454149718118888	25.397559409120102	23.190751445086704
17	22.32555484193249	27.484764147577252	26.527652893741525	23.662028116748736
18	22.766859344894026	27.403696567473062	27.725397844858346	22.104046242774565
19	24.73788624848355	26.43716548918861	27.34517947620067	21.479768786127167
20	23.95261542853065	26.35923785056733	27.55898094626418	22.129165774637833
21	22.788838935274388	27.275529865125243	27.64661385855991	22.289017341040463
22	22.704060515235852	26.860486690929854	27.603796474702065	22.831656319132236
23	22.67665738956683	26.74145436380504	28.134732034539354	22.447156212088775
24	22.91329479768786	27.447084849782343	26.45315064582887	23.186469706700922
25	23.059730250481696	26.86905016770142	27.72596874330978	22.3452508385071
26	22.98094626418326	27.235566973524584	27.48219510454578	22.30129165774638
27	22.97238278741169	27.085135231570685	26.971526439734532	22.970955541283093
28	22.589595375722542	26.852779561835437	27.91379433383287	22.64383072860915
29	23.875829586812245	26.797402412045955	26.939556126454008	22.38721187468779
30	23.450510240490974	26.573895668308	27.54556483265539	22.430029258545638
31	23.07514450867052	26.758581317348177	27.358595589809465	22.80767858417184
32	22.12003139941483	26.77513737243988	27.73082138014701	23.374009847998288
33	22.187968315135944	26.724612859487618	28.28659102262185	22.800827802754586
34	23.003782202240778	26.35409976450439	27.80161278812531	22.84050524512952
35	22.793120673660173	27.21130378933847	27.40712195818169	22.588453578819667
36	23.52101619924356	26.81852565474916	26.99407692856633	22.666381217440946
37	22.772853778634126	26.45086705202312	27.371440804966817	23.404838364375937
38	22.892171554984657	26.763433954185402	27.270391779062297	23.074002711767644
39	22.330407478769715	26.734889031613502	27.818739741668452	23.115963747948335
40	22.671804752729606	27.413687290373222	27.820166987797045	22.09434096910012
41	22.714622136587455	26.469135802469136	27.416256333404696	23.399985727538713
42	22.23306929279954	27.393705844572896	27.546992078783987	22.826232783843572
43	23.115392849496896	26.01184614286734	27.644330264754156	23.22843074288161
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	14.5
2	25.0
3	87.5
4	150.0
5	150.0
6	200.0
7	250.0
8	232.5
9	215.0
10	289.0
11	363.0
12	363.0
13	593.0
14	823.0
15	1398.0
16	1973.0
17	2234.5
18	2496.0
19	2496.0
20	2330.0
21	2164.0
22	2283.0
23	2402.0
24	3411.0
25	4420.0
26	4420.0
27	5687.5
28	6955.0
29	8443.0
30	9931.0
31	11805.0
32	13679.0
33	13679.0
34	16046.5
35	18414.0
36	20241.0
37	22068.0
38	23934.5
39	25801.0
40	25801.0
41	27271.0
42	28741.0
43	29049.0
44	29357.0
45	28781.0
46	28205.0
47	28205.0
48	27275.5
49	26346.0
50	25923.0
51	25500.0
52	24194.0
53	22888.0
54	22888.0
55	21894.0
56	20900.0
57	18094.5
58	15289.0
59	14188.5
60	13088.0
61	13088.0
62	11421.5
63	9755.0
64	8057.0
65	6359.0
66	5389.0
67	4419.0
68	4419.0
69	3695.0
70	2971.0
71	2409.0
72	1847.0
73	1489.0
74	1131.0
75	1131.0
76	857.0
77	583.0
78	464.5
79	346.0
80	273.0
81	200.0
82	200.0
83	165.5
84	131.0
85	107.5
86	84.0
87	56.0
88	28.0
89	28.0
90	24.5
91	21.0
92	11.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	350325.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.63555818026137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.22693909697253	57.02597455068745
2	6.522411198247063	8.431593769597727
3	1.8554042419365024	3.5977526648277163
4	0.8753199507698814	2.2630717441732076
5	0.5329050551738725	1.7222307849123117
6	0.34489188684387273	1.3375367770798354
7	0.24104430188597845	1.0906023099000153
8	0.18456570728886257	0.9543606009240294
9	0.13662516996139565	0.7947759709735097
>10	0.9263160974935262	11.782327996548284
>50	0.10372031700979989	4.654834550087457
>100	0.048521521691266	5.478306949786508
>500	4.4515157514922937E-4	0.15968804396699118
>1k	8.903031502984587E-4	0.7069432865349501
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.4087632912295725	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1025	0.2925854563619496	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.15842432027403125	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	440	0.1255976593163491	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	398	0.11360879183615213	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	377	0.10761435809605367	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	370	0.10561621351602084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.8544922571897526E-4	2.8544922571897526E-4	0.0	0.0	0.0
3	2.8544922571897526E-4	2.8544922571897526E-4	2.8544922571897526E-4	0.0	0.0
4	2.8544922571897526E-4	2.8544922571897526E-4	2.8544922571897526E-4	0.0	0.0
5	2.8544922571897526E-4	2.8544922571897526E-4	8.563476771569257E-4	0.0	0.0
6	2.8544922571897526E-4	2.8544922571897526E-4	0.002569043031470777	0.0	0.0
7	2.8544922571897526E-4	2.8544922571897526E-4	0.00542353528866053	0.0	0.0
8	2.8544922571897526E-4	2.8544922571897526E-4	0.0074216798686933564	0.0	0.0
9	2.8544922571897526E-4	2.8544922571897526E-4	0.009134375223007208	0.001141796902875901	0.0
10	8.563476771569257E-4	2.8544922571897526E-4	0.010561621351602084	0.001141796902875901	0.0
11	8.563476771569257E-4	2.8544922571897526E-4	0.014272461285948761	0.0017126953543138515	0.0
12	8.563476771569257E-4	2.8544922571897526E-4	0.015414258188824663	0.0019981445800328268	0.0
13	8.563476771569257E-4	2.8544922571897526E-4	0.01684150431741954	0.002283593805751802	0.0
14	8.563476771569257E-4	2.8544922571897526E-4	0.017697851994576463	0.002283593805751802	0.0
15	8.563476771569257E-4	2.8544922571897526E-4	0.019410547348890316	0.002283593805751802	0.0
16	8.563476771569257E-4	2.8544922571897526E-4	0.02026689502604724	0.002283593805751802	0.0
17	8.563476771569257E-4	2.8544922571897526E-4	0.020837793477485194	0.002283593805751802	0.0
18	8.563476771569257E-4	2.8544922571897526E-4	0.021408691928923144	0.002283593805751802	0.0
19	8.563476771569257E-4	2.8544922571897526E-4	0.021408691928923144	0.002283593805751802	0.0
20	8.563476771569257E-4	2.8544922571897526E-4	0.02226503960608007	0.002283593805751802	0.0
21	8.563476771569257E-4	2.8544922571897526E-4	0.022550488831799043	0.0028544922571897523	2.8544922571897526E-4
22	8.563476771569257E-4	2.8544922571897526E-4	0.022835938057518018	0.0028544922571897523	2.8544922571897526E-4
23	0.001141796902875901	2.8544922571897526E-4	0.022835938057518018	0.003425390708627703	2.8544922571897526E-4
24	0.001141796902875901	2.8544922571897526E-4	0.022835938057518018	0.0037108399343466782	2.8544922571897526E-4
25	0.001141796902875901	2.8544922571897526E-4	0.023121387283236993	0.0039962891600656535	2.8544922571897526E-4
26	0.001141796902875901	2.8544922571897526E-4	0.023121387283236993	0.005138086062941554	2.8544922571897526E-4
27	0.001141796902875901	2.8544922571897526E-4	0.023121387283236993	0.006850781417255406	2.8544922571897526E-4
28	0.001141796902875901	2.8544922571897526E-4	0.023121387283236993	0.01313066438307286	2.8544922571897526E-4
29	0.001141796902875901	5.708984514379505E-4	0.023121387283236993	0.028259473346178548	2.8544922571897526E-4
30	0.001141796902875901	5.708984514379505E-4	0.023121387283236993	0.04995361450082066	2.8544922571897526E-4
31	0.001141796902875901	5.708984514379505E-4	0.023121387283236993	0.11446513951330907	2.8544922571897526E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATGC	25	0.0054911915	29.6	3
GTATCAA	680	0.0	24.485294	1
TGCGGGT	40	0.0019285224	23.125	21
TATACCC	40	0.0019285224	23.125	4
GGTATCA	230	0.0	21.717392	1
GAGTTAG	45	0.0038205802	20.555555	1
CCGGTGC	45	0.0038205802	20.555555	8
GTATAGA	75	9.235648E-6	19.733334	1
GTTAGGT	115	3.0286174E-9	19.304348	1
TCTGCGG	50	0.0070253583	18.5	19
CGGGTAA	50	0.0070253583	18.5	23
AGCCGGT	50	0.0070253583	18.5	6
TACCCTC	130	6.91216E-10	18.5	5
TCACCTG	60	9.2193513E-4	18.5	37
GGTAACG	50	0.0070253583	18.5	25
TATATAG	60	9.2193513E-4	18.5	2
ACGGGCG	50	0.0070253583	18.5	16
GCCGCTC	50	0.0070253583	18.5	27
ACTGTCA	170	0.0	18.5	12
GTATATA	50	0.0070253583	18.5	1
>>END_MODULE