FastQCFastQC Report
Fri 10 Feb 2017
ERR1633130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353416
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8080.2286257554836227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.17344998528646127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1500.027.1333331
CGAACCG358.857194E-426.42857231
ATCGGTC451.3206802E-424.66666814
TATCGGT451.3206802E-424.66666813
GTATCAA4250.023.070591
TTAGACT502.6969737E-422.24
GGCGAAC450.003820644720.55555729
AATAGCG555.133412E-420.1818186
TGTTAAC759.236026E-619.7333342
TATACTG1355.4569682E-1119.1851865
ACTATCG609.2195696E-418.511
GCGAACC500.007025479318.530
TAGGTAC609.2195696E-418.53
ATAGCGG500.007025479318.57
ATAGCAC609.2195696E-418.53
ATACTGC701.21616766E-418.56
TAGCACC609.2195696E-418.54
TCTAATA801.6115133E-518.52
TCTATAC902.1436463E-618.53
GAGTATT752.0621662E-417.26666627
Produced by FastQC (version 0.11.5)