##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353416
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.465918351178214	33.0	33.0	33.0	27.0	33.0
2	31.0501024288657	33.0	33.0	33.0	27.0	33.0
3	31.48725864137447	33.0	33.0	33.0	27.0	33.0
4	31.635910654865654	33.0	33.0	33.0	27.0	33.0
5	31.74016456527152	33.0	33.0	33.0	27.0	33.0
6	34.55467777350205	37.0	37.0	37.0	27.0	37.0
7	34.72996129207506	37.0	37.0	37.0	27.0	37.0
8	34.899526337234306	37.0	37.0	37.0	27.0	37.0
9	34.9686488444213	37.0	37.0	37.0	27.0	37.0
10	35.01763926930303	37.0	37.0	37.0	27.0	37.0
11	35.05267446861489	37.0	37.0	37.0	27.0	37.0
12	35.01545770423524	37.0	37.0	37.0	27.0	37.0
13	35.03654050750391	37.0	37.0	37.0	27.0	37.0
14	35.012175453290176	37.0	37.0	37.0	27.0	37.0
15	35.039681282115126	37.0	37.0	37.0	27.0	37.0
16	35.03495314303823	37.0	37.0	37.0	27.0	37.0
17	35.00624759490233	37.0	37.0	37.0	27.0	37.0
18	35.001867487606674	37.0	37.0	37.0	27.0	37.0
19	34.999241686850624	37.0	37.0	37.0	27.0	37.0
20	34.99691015686896	37.0	37.0	37.0	27.0	37.0
21	34.98820936233787	37.0	37.0	37.0	27.0	37.0
22	34.97965853272065	37.0	37.0	37.0	27.0	37.0
23	34.99943409466464	37.0	37.0	37.0	27.0	37.0
24	34.97697897095774	37.0	37.0	37.0	27.0	37.0
25	34.95332695746656	37.0	37.0	37.0	27.0	37.0
26	34.92712271091292	37.0	37.0	37.0	27.0	37.0
27	34.848444892138446	37.0	37.0	37.0	27.0	37.0
28	34.83362666093216	37.0	37.0	37.0	27.0	37.0
29	34.863438553998684	37.0	37.0	37.0	27.0	37.0
30	34.81571858659483	37.0	37.0	37.0	27.0	37.0
31	34.83638261991534	37.0	37.0	37.0	27.0	37.0
32	34.8591235258166	37.0	37.0	37.0	27.0	37.0
33	34.82123333408787	37.0	37.0	37.0	27.0	37.0
34	34.751004481970256	37.0	37.0	37.0	27.0	37.0
35	34.75703986237182	37.0	37.0	37.0	27.0	37.0
36	34.72737793421916	37.0	37.0	37.0	27.0	37.0
37	34.756686171537226	37.0	37.0	37.0	27.0	37.0
38	34.627410756728615	37.0	37.0	37.0	27.0	37.0
39	34.692051293659596	37.0	37.0	37.0	27.0	37.0
40	34.7286653688571	37.0	37.0	37.0	27.0	37.0
41	34.735733526495686	37.0	37.0	37.0	27.0	37.0
42	34.66732406003124	37.0	37.0	37.0	27.0	37.0
43	34.425362745319966	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	15.0
17	20.0
18	14.0
19	21.0
20	35.0
21	103.0
22	314.0
23	742.0
24	1361.0
25	2378.0
26	3695.0
27	5470.0
28	7407.0
29	9761.0
30	12333.0
31	15314.0
32	19369.0
33	24885.0
34	34944.0
35	61373.0
36	153855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.91302034995586	21.02847635647509	13.786302827263055	25.272200466305993
2	15.669918735993843	23.094313783190348	37.537349752133466	23.698417728682347
3	18.045872286483917	26.636881182515786	29.98704076782036	25.330205763179936
4	13.255200670031916	18.159619258890373	38.437705140684066	30.147474930393642
5	14.42492699821174	36.92447427394345	35.05840143060869	13.592197297236119
6	30.16501799578967	39.20082848541096	17.626253480317814	13.007900038481562
7	26.633485750503656	31.876881635240057	23.79264096701904	17.69699164723725
8	24.638386490707834	36.28726486633316	20.779478008918666	18.29487063404034
9	25.708513479865086	15.6704846413292	20.306947053896824	38.31405482490889
10	14.62355977092152	28.51851642257283	34.275188446476676	22.582735360028973
11	33.99138692079589	23.644090816488216	24.345247526993685	18.019274735722206
12	22.523315299816645	25.439991398238902	30.85740317359712	21.17929012834733
13	28.212361636145506	21.81480181995156	26.3810919709351	23.591744572967833
14	21.880729791520476	20.668843515856665	27.615331507345452	29.835095185277403
15	23.28530683387283	29.156008782850805	23.792075061683683	23.766609321592682
16	23.289834076555675	28.197648097426264	25.211648595422957	23.300869230595104
17	22.42626253480318	27.87960929895647	26.785431333046606	22.908696833193744
18	23.23522421169387	27.49507662358241	27.198542227856127	22.0711569368676
19	24.004572515109672	27.04178644996265	26.98802544310388	21.965615591823802
20	23.717375557416755	26.90115897412681	26.989723159109946	22.391742309346494
21	23.372456255517577	27.02282862122824	26.906535074812687	22.698180048441497
22	23.009993888222375	26.875693234035808	27.160626570387304	22.953686307354506
23	22.89171967313308	26.650462910564322	27.36406953844761	23.09374787785499
24	22.84984267831677	27.13883921497612	26.738178237544425	23.273139869162687
25	23.27540349050411	26.797032392421393	27.136858546302374	22.790705570772122
26	23.056964031056886	27.02933653258483	27.087624782126447	22.82607465423184
27	22.986508816805124	27.099508794168912	26.89861240011771	23.015369988908255
28	23.120628381284376	26.795617629083008	27.209577381895556	22.874176607737056
29	23.276252348507143	26.950675690970417	26.895499920773254	22.877572039749193
30	23.283326165199085	26.9673698983634	26.84428548792358	22.905018448513932
31	23.522421169386785	26.564445299590282	27.049992077325307	22.863141453697626
32	22.778821558729657	26.899744210788416	27.071496480068813	23.24993775041311
33	22.996695112841522	26.734216900196934	27.252303234714898	23.016784752246643
34	23.351234805441745	26.388731692962402	27.114788238223507	23.145245263372345
35	22.971229372750525	27.004153745161506	27.246361228693665	22.7782556533943
36	23.58099237159608	26.87371256536207	26.5819883649863	22.96330669805555
37	23.180614346832062	26.679607035335128	26.877673902709553	23.262104715123254
38	23.123740860628832	27.064988568712227	26.905120311474295	22.906150259184642
39	22.774577268714488	26.885313624736856	27.28597460216855	23.05413450438011
40	23.025273332276978	26.716956787468593	27.429997510016523	22.827772370237906
41	22.96443850872626	26.41957353373928	27.486588043552075	23.12939991398239
42	22.87389365506938	26.843153677252868	27.11309052221744	23.169862145460307
43	23.12317495529348	26.373452248907803	27.407078343934625	23.09629445186409
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	15.0
2	22.0
3	66.5
4	111.0
5	111.0
6	121.0
7	131.0
8	127.0
9	123.0
10	169.5
11	216.0
12	216.0
13	356.0
14	496.0
15	879.5
16	1263.0
17	1476.5
18	1690.0
19	1690.0
20	1870.5
21	2051.0
22	2503.0
23	2955.0
24	3946.0
25	4937.0
26	4937.0
27	6392.0
28	7847.0
29	9540.5
30	11234.0
31	12987.5
32	14741.0
33	14741.0
34	16889.5
35	19038.0
36	20670.5
37	22303.0
38	23550.5
39	24798.0
40	24798.0
41	25884.0
42	26970.0
43	27235.0
44	27500.0
45	27594.0
46	27688.0
47	27688.0
48	27030.5
49	26373.0
50	25732.5
51	25092.0
52	23995.5
53	22899.0
54	22899.0
55	21522.0
56	20145.0
57	18367.0
58	16589.0
59	15381.0
60	14173.0
61	14173.0
62	12488.5
63	10804.0
64	9176.0
65	7548.0
66	6319.0
67	5090.0
68	5090.0
69	4222.0
70	3354.0
71	2762.0
72	2170.0
73	1740.5
74	1311.0
75	1311.0
76	1032.0
77	753.0
78	595.5
79	438.0
80	341.5
81	245.0
82	245.0
83	210.5
84	176.0
85	128.5
86	81.0
87	59.0
88	37.0
89	37.0
90	25.0
91	13.0
92	8.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	353416.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.2610774673812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8605086878152	71.22440740363201
2	6.32721079659197	10.030030902022535
3	1.6116454705938459	3.832222694840785
4	0.7139159337076022	2.2634298450718413
5	0.396024997165185	1.5694683989664573
6	0.23907534212414894	1.1369621527585698
7	0.1655205366246369	0.9183535253093498
8	0.12717110238813323	0.8063774878398475
9	0.08737731123701456	0.623305785136364
>10	0.4550780506050322	6.260638053104079
>50	0.014323279259038202	0.7251591718146566
>100	0.0014323279254292802	0.20633687583754096
>500	7.161639627146401E-4	0.40330770366595015
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.2286257554836227	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	613	0.17344998528646127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.8295266767775087E-4	0.0	0.0
2	0.0	0.0	2.8295266767775087E-4	0.0	0.0
3	0.0	0.0	2.8295266767775087E-4	0.0	0.0
4	0.0	0.0	2.8295266767775087E-4	0.0	0.0
5	0.0	0.0	5.659053353555017E-4	0.0	0.0
6	0.0	0.0	5.659053353555017E-4	0.0	0.0
7	0.0	0.0	0.0019806686737442562	0.0	0.0
8	0.0	0.0	0.0025465740090997576	0.0	0.0
9	0.0	0.0	0.0028295266767775088	2.8295266767775087E-4	0.0
10	0.0	0.0	0.0036783846798107613	2.8295266767775087E-4	0.0
11	0.0	0.0	0.005942006021232768	2.8295266767775087E-4	0.0
12	0.0	0.0	0.00650791135658827	2.8295266767775087E-4	0.0
13	0.0	0.0	0.007356769359621523	2.8295266767775087E-4	0.0
14	0.0	0.0	0.008205627362654774	5.659053353555017E-4	0.0
15	0.0	0.0	0.00962039070104353	5.659053353555017E-4	0.0
16	0.0	0.0	0.009903343368721281	5.659053353555017E-4	0.0
17	0.0	0.0	0.010752201371754533	5.659053353555017E-4	0.0
18	0.0	0.0	0.011035154039432284	5.659053353555017E-4	0.0
19	0.0	0.0	0.011601059374787786	5.659053353555017E-4	0.0
20	0.0	0.0	0.011601059374787786	5.659053353555017E-4	0.0
21	0.0	0.0	0.011884012042465536	8.488580030332525E-4	0.0
22	0.0	0.0	0.011884012042465536	0.0011318106707110035	0.0
23	0.0	0.0	0.011884012042465536	0.0014147633383887544	0.0
24	0.0	0.0	0.012166964710143287	0.0014147633383887544	0.0
25	0.0	0.0	0.012449917377821038	0.0014147633383887544	0.0
26	0.0	0.0	0.012449917377821038	0.0014147633383887544	0.0
27	0.0	0.0	0.012449917377821038	0.001697716006066505	0.0
28	0.0	0.0	0.012449917377821038	0.0070738166919437715	0.0
29	0.0	0.0	0.012449917377821038	0.017826018063698306	0.0
30	0.0	0.0	0.012449917377821038	0.03452022545668561	0.0
31	0.0	0.0	0.012449917377821038	0.0823392262942255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	150	0.0	27.133333	1
CGAACCG	35	8.857194E-4	26.428572	31
ATCGGTC	45	1.3206802E-4	24.666668	14
TATCGGT	45	1.3206802E-4	24.666668	13
GTATCAA	425	0.0	23.07059	1
TTAGACT	50	2.6969737E-4	22.2	4
GGCGAAC	45	0.0038206447	20.555557	29
AATAGCG	55	5.133412E-4	20.181818	6
TGTTAAC	75	9.236026E-6	19.733334	2
TATACTG	135	5.4569682E-11	19.185186	5
ACTATCG	60	9.2195696E-4	18.5	11
GCGAACC	50	0.0070254793	18.5	30
TAGGTAC	60	9.2195696E-4	18.5	3
ATAGCGG	50	0.0070254793	18.5	7
ATAGCAC	60	9.2195696E-4	18.5	3
ATACTGC	70	1.21616766E-4	18.5	6
TAGCACC	60	9.2195696E-4	18.5	4
TCTAATA	80	1.6115133E-5	18.5	2
TCTATAC	90	2.1436463E-6	18.5	3
GAGTATT	75	2.0621662E-4	17.266666	27
>>END_MODULE