FastQCFastQC Report
Fri 10 Feb 2017
ERR1633129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384900
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11520.299298519095869No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8120.21096388672382435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.20135100025980776No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7400.19225772928033255No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7320.1901792673421668No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7180.18654195895037673No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6320.16419849311509482No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6180.16056118472330475No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6150.15978176149649262No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6110.15874253052740972No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5760.1496492595479345No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5560.14445310470252015No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5510.14315406599116653No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4970.12912444790854768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4950.12860483242400625No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4780.124188100805404No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4770.12392829306313329No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4660.12107040789815536No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4630.1202909846713432No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4610.11977136918680177No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4450.11561444531047024No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4210.10937905949597299No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4140.10756040530007795No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4080.10600155884645363No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC3960.10288386593920498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAA303.5950704E-430.83333426
CGATAAC250.005491859329.59999815
GTCTATA451.3209796E-424.6666661
GGTATCA2300.024.1304361
GTATCAA6750.024.1185191
CCGGCGT400.001928866523.12500223
GGCGTCA400.001928866523.12500225
ATCGCTA400.001928866523.12500210
ATCGCGC502.6975802E-422.1999998
GCCGAGT450.003821255920.55555512
CGCTATC450.003821255920.55555512
CGTCAAA450.003821255920.55555527
ATTAGAG2150.018.9302333
TATTAGA2350.018.8936162
GTAATAC1205.142283E-918.53
GACTGTC609.2216075E-418.57
TCTATAC609.2216075E-418.53
CGGCGTC500.00702659218.49999824
GCACCGC2000.018.49999810
TGTGACG500.00702659218.49999810
Produced by FastQC (version 0.11.5)