##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633129.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384900
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69812938425565	33.0	33.0	33.0	27.0	33.0
2	31.24303975058457	33.0	33.0	33.0	27.0	33.0
3	31.669062094050403	33.0	33.0	33.0	27.0	33.0
4	31.803593141075606	33.0	33.0	33.0	27.0	33.0
5	31.91102883865939	33.0	33.0	33.0	33.0	33.0
6	34.79452065471551	37.0	37.0	37.0	27.0	37.0
7	34.92660431280852	37.0	37.0	37.0	27.0	37.0
8	35.113551571836844	37.0	37.0	37.0	33.0	37.0
9	35.1688672382437	37.0	37.0	37.0	33.0	37.0
10	35.24244738893219	37.0	37.0	37.0	33.0	37.0
11	35.25847233047545	37.0	37.0	37.0	33.0	37.0
12	35.186599116653674	37.0	37.0	37.0	33.0	37.0
13	35.22313587944921	37.0	37.0	37.0	33.0	37.0
14	35.180166276955056	37.0	37.0	37.0	33.0	37.0
15	35.22114835022084	37.0	37.0	37.0	33.0	37.0
16	35.213497012210965	37.0	37.0	37.0	33.0	37.0
17	35.19835801506885	37.0	37.0	37.0	33.0	37.0
18	35.19693426864121	37.0	37.0	37.0	33.0	37.0
19	35.21734216679657	37.0	37.0	37.0	33.0	37.0
20	35.10929332294102	37.0	37.0	37.0	33.0	37.0
21	35.191073005975575	37.0	37.0	37.0	33.0	37.0
22	35.20791634190699	37.0	37.0	37.0	33.0	37.0
23	35.200898934788256	37.0	37.0	37.0	33.0	37.0
24	35.18406339308911	37.0	37.0	37.0	33.0	37.0
25	35.18314367368148	37.0	37.0	37.0	33.0	37.0
26	35.10517796830346	37.0	37.0	37.0	33.0	37.0
27	35.066596518576254	37.0	37.0	37.0	33.0	37.0
28	35.08527929332294	37.0	37.0	37.0	33.0	37.0
29	35.11357495453365	37.0	37.0	37.0	33.0	37.0
30	35.08111457521434	37.0	37.0	37.0	33.0	37.0
31	35.04719927253832	37.0	37.0	37.0	27.0	37.0
32	35.09848791893999	37.0	37.0	37.0	33.0	37.0
33	35.08465315666407	37.0	37.0	37.0	33.0	37.0
34	35.03015848272278	37.0	37.0	37.0	27.0	37.0
35	35.0187191478306	37.0	37.0	37.0	27.0	37.0
36	34.997799428422965	37.0	37.0	37.0	27.0	37.0
37	35.01864640166277	37.0	37.0	37.0	27.0	37.0
38	34.856965445570275	37.0	37.0	37.0	27.0	37.0
39	34.927622759158226	37.0	37.0	37.0	27.0	37.0
40	34.95926214601195	37.0	37.0	37.0	27.0	37.0
41	34.99682255131203	37.0	37.0	37.0	27.0	37.0
42	34.86646661470512	37.0	37.0	37.0	27.0	37.0
43	34.67485320862562	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	13.0
16	14.0
17	13.0
18	10.0
19	10.0
20	35.0
21	85.0
22	211.0
23	577.0
24	1078.0
25	2021.0
26	3176.0
27	4886.0
28	6961.0
29	9275.0
30	12208.0
31	15336.0
32	19755.0
33	25391.0
34	36309.0
35	65951.0
36	181585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.92751363990647	21.66692647440894	13.710574175110418	24.694985710574173
2	15.694466095089634	23.101844634970124	38.40322161600416	22.80046765393609
3	17.010132501948558	27.94466095089634	30.282151208106	24.763055339049103
4	12.18394388152767	18.162899454403743	40.3096908287867	29.34346583528189
5	13.282670823590543	37.4143933489218	36.599636269160825	12.703299558326838
6	28.796570537802026	41.25123408677578	17.25149389451806	12.700701480904131
7	26.064172512340868	32.427123928293064	24.49415432579891	17.014549233567163
8	23.49857105741751	36.75370226032736	20.991945959989607	18.755780722265524
9	25.531826448428163	15.434398545076643	20.79085476747207	38.24292023902312
10	14.66069108859444	28.3912704598597	34.97116134060795	21.976877110937906
11	33.252273317744866	24.254871395167577	24.394128345024683	18.098726942062875
12	22.591062613665887	26.46453624318005	30.182644842816313	20.76175630033775
13	26.535463756819954	23.045206547155107	26.6406858924396	23.778643803585346
14	21.576513380098728	21.154065991166537	27.8695765133801	29.39984411535464
15	23.29955832683814	29.212522733177448	25.304754481683556	22.18316445830086
16	22.821771888802285	28.937126526370488	25.71369186801767	22.52740971680956
17	21.943361912184983	28.123408677578592	26.58820472850091	23.345024681735516
18	22.077422707196675	28.4081579631073	28.200051961548457	21.314367368147572
19	24.01766692647441	26.798908807482462	27.491816056118473	21.691608209924656
20	23.21641984931151	27.00103923096908	28.236165237724087	21.546375681995322
21	21.88100805404001	28.054559625876852	27.989867498051442	22.074564822031697
22	22.025980774227072	27.835541699142635	27.53468433359314	22.603793193037152
23	22.10522213561964	27.72642244738893	28.393868537282412	21.774486879709016
24	22.67420109119252	28.389451805663807	26.068589243959472	22.867757859184206
25	22.83787996882307	27.504286827747464	28.060535203949076	21.597297999480382
26	22.62483762016108	27.85476747207067	27.684073785398805	21.836321122369444
27	22.522733177448686	27.781241881008057	26.763834762275916	22.93219017926734
28	22.044686931670565	27.288906209405038	28.545856066510783	22.120550792413614
29	23.7329176409457	27.219017926734217	27.37879968823071	21.66926474408937
30	22.818134580410497	27.002598077422707	27.909846713432064	22.269420628734736
31	22.478825669004937	27.4551831644583	27.80904130943102	22.25694985710574
32	21.587165497531828	27.183684073785397	28.06105481943362	23.168095609249157
33	21.828526890101323	27.270979475188362	28.570018186541958	22.330475448168354
34	22.78254091971941	26.798648999740195	28.546375681995322	21.872434398545078
35	22.422967004416734	27.68303455442972	27.54221875811899	22.351779683034554
36	23.088594440114317	27.6752403221616	27.096128864640168	22.14003637308392
37	22.159781761496493	26.769030917121327	27.749025720966486	23.32216160041569
38	22.427383736035335	27.449987009612887	27.492335671603012	22.630293582748767
39	21.865679397246037	27.5705378020265	28.319043907508444	22.24473889321902
40	22.132242140815798	28.088854247856588	28.120550792413614	21.658352818914004
41	22.349181605611847	26.89166017147311	27.644323200831384	23.114835022083657
42	21.835801506884906	27.910626136658873	27.82956612107041	22.424006235385814
43	22.983891919979214	26.468952974798647	27.815016887503248	22.73213821771889
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	11.5
2	18.0
3	73.0
4	128.0
5	128.0
6	169.0
7	210.0
8	188.5
9	167.0
10	229.0
11	291.0
12	291.0
13	437.5
14	584.0
15	1031.0
16	1478.0
17	1833.0
18	2188.0
19	2188.0
20	2221.5
21	2255.0
22	2656.5
23	3058.0
24	4007.0
25	4956.0
26	4956.0
27	6383.0
28	7810.0
29	9639.5
30	11469.0
31	13772.5
32	16076.0
33	16076.0
34	19092.5
35	22109.0
36	24565.0
37	27021.0
38	30056.5
39	33092.0
40	33092.0
41	34100.0
42	35108.0
43	35880.0
44	36652.0
45	34459.0
46	32266.0
47	32266.0
48	30235.0
49	28204.0
50	27716.0
51	27228.0
52	24535.5
53	21843.0
54	21843.0
55	21274.5
56	20706.0
57	17355.0
58	14004.0
59	12913.0
60	11822.0
61	11822.0
62	10379.0
63	8936.0
64	7293.5
65	5651.0
66	4646.5
67	3642.0
68	3642.0
69	2984.5
70	2327.0
71	1915.5
72	1504.0
73	1213.5
74	923.0
75	923.0
76	704.5
77	486.0
78	378.5
79	271.0
80	225.0
81	179.0
82	179.0
83	140.0
84	101.0
85	87.5
86	74.0
87	57.5
88	41.0
89	41.0
90	27.0
91	13.0
92	8.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	384900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.10048557369565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.19277658873851	52.12235920486904
2	6.31370999347012	7.462866527711576
3	1.80607535193869	3.202197908467795
4	0.8667542984001572	2.049023996341487
5	0.5281717242496643	1.560760268472562
6	0.3409828335309117	1.2091350620382908
7	0.2583705279810907	1.0688876563130205
8	0.20859157971638365	0.9862290918258014
9	0.15393511952884764	0.8187876279899798
>10	1.1126662093910813	12.668312139010652
>50	0.12936180026818658	5.372425971746464
>100	0.08284471455527577	9.099531761880069
>500	0.005316238367183472	2.0778582051643046
>1k	4.4301986393195604E-4	0.3016245781690119
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.299298519095869	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	812	0.21096388672382435	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.20135100025980776	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	740	0.19225772928033255	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	732	0.1901792673421668	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	718	0.18654195895037673	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	632	0.16419849311509482	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	618	0.16056118472330475	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	615	0.15978176149649262	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	611	0.15874253052740972	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	576	0.1496492595479345	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	556	0.14445310470252015	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	551	0.14315406599116653	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	497	0.12912444790854768	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	495	0.12860483242400625	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	478	0.124188100805404	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	477	0.12392829306313329	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	466	0.12107040789815536	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	463	0.1202909846713432	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	461	0.11977136918680177	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	445	0.11561444531047024	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	421	0.10937905949597299	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	414	0.10756040530007795	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	408	0.10600155884645363	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	396	0.10288386593920498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	5.196154845414394E-4	0.0	0.0
7	0.0	0.0	0.0012990387113535984	0.0	0.0
8	0.0	0.0	0.0018186541958950377	0.0	0.0
9	0.0	0.0	0.0020784619381657575	7.79423226812159E-4	0.0
10	0.0	0.0	0.0028578851649779164	7.79423226812159E-4	0.0
11	0.0	0.0	0.004156923876331515	7.79423226812159E-4	0.0
12	0.0	0.0	0.004676539360872954	7.79423226812159E-4	0.0
13	0.0	0.0	0.004936347103143674	7.79423226812159E-4	0.0
14	0.0	0.0	0.005196154845414394	7.79423226812159E-4	0.0
15	0.0	0.0	0.005455962587685113	7.79423226812159E-4	0.0
16	0.0	0.0	0.006235385814497272	0.0010392309690828788	0.0
17	0.0	0.0	0.006495193556767992	0.0010392309690828788	0.0
18	0.0	0.0	0.006755001299038711	0.0012990387113535984	0.0
19	0.0	0.0	0.006755001299038711	0.0012990387113535984	0.0
20	0.0	0.0	0.00753442452585087	0.0012990387113535984	0.0
21	0.0	0.0	0.00857365549493375	0.001558846453624318	0.0
22	0.0	0.0	0.00883346323720447	0.0018186541958950377	0.0
23	0.0	0.0	0.00883346323720447	0.0018186541958950377	0.0
24	0.0	0.0	0.00883346323720447	0.0020784619381657575	0.0
25	0.0	0.0	0.00883346323720447	0.0020784619381657575	0.0
26	0.0	0.0	0.00883346323720447	0.002598077422707197	0.0
27	0.0	0.0	0.00883346323720447	0.003117692907248636	0.0
28	0.0	0.0	0.009093270979475189	0.009093270979475189	0.0
29	0.0	0.0	0.009353078721745909	0.021823850350740453	0.0
30	0.0	0.0	0.009353078721745909	0.03949077682514939	0.0
31	0.0	0.0	0.009353078721745909	0.08781501688750325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAA	30	3.5950704E-4	30.833334	26
CGATAAC	25	0.0054918593	29.599998	15
GTCTATA	45	1.3209796E-4	24.666666	1
GGTATCA	230	0.0	24.130436	1
GTATCAA	675	0.0	24.118519	1
CCGGCGT	40	0.0019288665	23.125002	23
GGCGTCA	40	0.0019288665	23.125002	25
ATCGCTA	40	0.0019288665	23.125002	10
ATCGCGC	50	2.6975802E-4	22.199999	8
GCCGAGT	45	0.0038212559	20.555555	12
CGCTATC	45	0.0038212559	20.555555	12
CGTCAAA	45	0.0038212559	20.555555	27
ATTAGAG	215	0.0	18.930233	3
TATTAGA	235	0.0	18.893616	2
GTAATAC	120	5.142283E-9	18.5	3
GACTGTC	60	9.2216075E-4	18.5	7
TCTATAC	60	9.2216075E-4	18.5	3
CGGCGTC	50	0.007026592	18.499998	24
GCACCGC	200	0.0	18.499998	10
TGTGACG	50	0.007026592	18.499998	10
>>END_MODULE