FastQCFastQC Report
Fri 10 Feb 2017
ERR1633128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277200
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10140.3658008658008658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.2813852813852814No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4120.14862914862914864No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3980.14357864357864358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3850.1388888888888889No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3540.1277056277056277No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC3540.1277056277056277No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3410.12301587301587302No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA3020.10894660894660896No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2870.10353535353535354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAA200.001839527737.026
CGTGCGT250.005489239529.614
CCCACCG250.005489239529.619
TGTACCG250.005489239529.65
GTGCGTA250.005489239529.615
GCCCGTA250.005489239529.626
GCACGGA358.852356E-426.4285721
CGGGAAC358.852356E-426.4285717
CGGAGTT358.852356E-426.4285724
GGTATCA1800.025.6944451
GTACCGG400.001927512223.1256
CGTATTA400.001927512223.1252
CACGGAG400.001927512223.12522
CCGTATT400.001927512223.1251
CGAGGGA400.001927512223.12536
GTATCAA5050.023.0792061
AGGACGA502.6949402E-422.232
TTAACGG502.6949402E-422.235
CATTCGG753.719615E-722.19999937
TCGCCAT855.1450115E-821.76470613
Produced by FastQC (version 0.11.5)