FastQCFastQC Report
Fri 10 Feb 2017
ERR1633127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367003
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13880.378198543336158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.28719111287918625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5260.1433230791028956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2450.030.204081
CGGCAAG453.99849E-628.77777718
GTATCAA6600.023.2651521
GACAGCG400.001928697123.1257
GCCCCGT502.6972484E-422.214
CGGTTTA502.6972484E-422.215
TAGAACA1001.2825694E-820.354
GGTACCG555.133929E-420.1818186
TAGGACC555.133929E-420.1818184
TAGTATG555.133929E-420.18181823
TGGCGGC656.88578E-519.92307715
CTAGAAC902.1441047E-618.53
CGGCCGT500.00702598318.513
GTCCTAC609.220494E-418.51
GTCTAAG609.220494E-418.51
CCATCGG500.00702598318.51
ATATAGG609.220494E-418.51
GTACTAC500.00702598318.51
CGCAAGG500.00702598318.512
GCCGTTA500.00702598318.515
Produced by FastQC (version 0.11.5)