##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633127.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367003
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.709890654844784	33.0	33.0	33.0	27.0	33.0
2	31.26259730846887	33.0	33.0	33.0	27.0	33.0
3	31.682828205763986	33.0	33.0	33.0	27.0	33.0
4	31.80516508039444	33.0	33.0	33.0	27.0	33.0
5	31.900801900801902	33.0	33.0	33.0	33.0	33.0
6	34.778862843083026	37.0	37.0	37.0	27.0	37.0
7	34.92383713484631	37.0	37.0	37.0	27.0	37.0
8	35.120197927537376	37.0	37.0	37.0	33.0	37.0
9	35.15420854870396	37.0	37.0	37.0	33.0	37.0
10	35.22449407770509	37.0	37.0	37.0	33.0	37.0
11	35.25827309313548	37.0	37.0	37.0	33.0	37.0
12	35.18831726171176	37.0	37.0	37.0	33.0	37.0
13	35.23829777958218	37.0	37.0	37.0	33.0	37.0
14	35.173835091266284	37.0	37.0	37.0	33.0	37.0
15	35.20508006746539	37.0	37.0	37.0	33.0	37.0
16	35.20749966621526	37.0	37.0	37.0	33.0	37.0
17	35.1998811998812	37.0	37.0	37.0	33.0	37.0
18	35.181042116821935	37.0	37.0	37.0	33.0	37.0
19	35.20573128830009	37.0	37.0	37.0	33.0	37.0
20	35.094309855777745	37.0	37.0	37.0	33.0	37.0
21	35.18746985719463	37.0	37.0	37.0	33.0	37.0
22	35.20853508009471	37.0	37.0	37.0	33.0	37.0
23	35.184121110726615	37.0	37.0	37.0	33.0	37.0
24	35.16690054304733	37.0	37.0	37.0	33.0	37.0
25	35.17137734568927	37.0	37.0	37.0	33.0	37.0
26	35.097661327019125	37.0	37.0	37.0	33.0	37.0
27	35.07727184791405	37.0	37.0	37.0	33.0	37.0
28	35.06822015078896	37.0	37.0	37.0	27.0	37.0
29	35.094653177221986	37.0	37.0	37.0	33.0	37.0
30	35.063449072623385	37.0	37.0	37.0	27.0	37.0
31	35.057514516230114	37.0	37.0	37.0	27.0	37.0
32	35.095661343367766	37.0	37.0	37.0	33.0	37.0
33	35.04710860674163	37.0	37.0	37.0	27.0	37.0
34	34.98976575123364	37.0	37.0	37.0	27.0	37.0
35	34.98954504459092	37.0	37.0	37.0	27.0	37.0
36	34.958673362343085	37.0	37.0	37.0	27.0	37.0
37	34.98990743944872	37.0	37.0	37.0	27.0	37.0
38	34.83994953719724	37.0	37.0	37.0	27.0	37.0
39	34.89050770702147	37.0	37.0	37.0	27.0	37.0
40	34.94746091993798	37.0	37.0	37.0	27.0	37.0
41	34.964624267376564	37.0	37.0	37.0	27.0	37.0
42	34.83886507739719	37.0	37.0	37.0	27.0	37.0
43	34.65449873706754	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	8.0
16	24.0
17	28.0
18	15.0
19	19.0
20	25.0
21	94.0
22	226.0
23	571.0
24	1050.0
25	2075.0
26	3232.0
27	4743.0
28	6621.0
29	8869.0
30	11457.0
31	14667.0
32	18540.0
33	24242.0
34	34893.0
35	62487.0
36	173117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08659329760248	21.334702986079133	14.04102963735991	24.53767407895848
2	15.465541153614549	23.521333613076735	38.720664408737804	22.292460824570917
3	17.371520123813703	27.118034457483997	31.369498341975405	24.140947076726892
4	12.470197791298709	18.305844911349496	39.792590251305846	29.43136704604595
5	13.39852807742716	37.726122129791854	35.90243131527535	12.972918477505631
6	28.59567905439465	40.52773410571576	18.01729141178682	12.859295428102769
7	25.274452797388573	32.996732996732995	24.176914085170967	17.55190012070746
8	23.672831012280554	36.72149818938809	21.262768969190986	18.342901829140363
9	25.363825363825367	15.87098743062046	20.84478873469699	37.92039847085719
10	14.182717852442622	28.827012313250844	34.86320275311101	22.127067081195523
11	32.8204401598897	24.189992997332446	25.045299357225964	17.94426748555189
12	21.80745116524933	26.134118794669252	31.290752391669823	20.767677648411592
13	27.23792448563091	22.754037432936517	27.46190085639627	22.546137225036308
14	21.57557295171974	21.81753282670714	28.473064252880764	28.133829968692353
15	23.17882960084795	29.304665084481595	25.053746154663585	22.46275916000687
16	22.324340672964528	29.606569973542452	25.495704394786962	22.57338495870606
17	21.7889227063539	28.504399146600985	27.200322613166648	22.50635553387847
18	21.992735754203643	28.08369413874001	28.574153344795544	21.349416762260798
19	23.87991378817067	26.938471892600337	28.454535793985336	20.727078525243662
20	23.62814472906216	26.814767181739658	28.506034010621168	21.051054078577014
21	22.17992768451484	27.74309746786811	27.810399370032396	22.266575477584652
22	21.853227357814514	27.769800246864467	27.98151513747844	22.39545725784258
23	21.79219243439427	27.74336994520481	28.47170186619728	21.992735754203643
24	22.41616553543159	27.74827453726536	27.643370762636817	22.192189164666228
25	22.489461939003224	27.441465056143954	28.264619090307164	21.804453914545658
26	22.321615899597553	27.6014092527854	28.032468399440873	22.044506448176175
27	22.39545725784258	27.671708405653362	27.46762288046692	22.465211456037142
28	22.011536690435772	27.642553330626722	28.346089813979724	21.99982016495778
29	22.739868611428243	27.632199191832218	27.71884698490203	21.909085211837507
30	22.402541668596715	27.850181061190238	28.041732628888592	21.705544641324458
31	22.490824325686713	27.46053846971278	27.892142571041656	22.156494633558854
32	21.623528962978504	27.47361738187426	28.193774982765806	22.709078672381423
33	21.680476726348285	27.5469137854459	28.73219020008011	22.040419288125708
34	22.191371732656137	27.319122731966765	27.932741694209582	22.55676384116751
35	22.092462459434937	27.993776617629827	27.986147252202297	21.92761367073294
36	22.494911485737173	27.602771639468887	27.804404868625056	22.097912006168887
37	22.717525469819048	27.02784445903712	27.762443358773638	22.4921867123702
38	22.909894469527497	27.43220082669624	27.751544265305732	21.90636043847053
39	22.142870766723977	27.46053846971278	28.248270450105313	22.14832031345793
40	22.0526807682771	27.661081789522157	28.237643833974108	22.048593608226636
41	21.866578747312694	27.0632665128078	28.558894613940485	22.511260125939025
42	22.289191096530548	27.413944845137507	27.98042522813165	22.316438830200298
43	22.213714874265335	26.8098625896791	28.34282008593935	22.63360245011621
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	20.5
2	25.0
3	93.5
4	162.0
5	162.0
6	203.0
7	244.0
8	217.5
9	191.0
10	287.5
11	384.0
12	384.0
13	575.5
14	767.0
15	1502.5
16	2238.0
17	2614.5
18	2991.0
19	2991.0
20	3209.0
21	3427.0
22	4202.5
23	4978.0
24	6226.5
25	7475.0
26	7475.0
27	8905.5
28	10336.0
29	12385.0
30	14434.0
31	16009.0
32	17584.0
33	17584.0
34	19119.0
35	20654.0
36	22305.0
37	23956.0
38	25310.0
39	26664.0
40	26664.0
41	27370.5
42	28077.0
43	27589.0
44	27101.0
45	26811.5
46	26522.0
47	26522.0
48	25620.5
49	24719.0
50	25060.5
51	25402.0
52	25412.0
53	25422.0
54	25422.0
55	22757.5
56	20093.0
57	17922.5
58	15752.0
59	13705.5
60	11659.0
61	11659.0
62	10343.0
63	9027.0
64	7449.5
65	5872.0
66	4903.0
67	3934.0
68	3934.0
69	3246.0
70	2558.0
71	2216.5
72	1875.0
73	1504.0
74	1133.0
75	1133.0
76	860.0
77	587.0
78	434.0
79	281.0
80	235.5
81	190.0
82	190.0
83	153.5
84	117.0
85	103.5
86	90.0
87	66.5
88	43.0
89	43.0
90	30.5
91	18.0
92	11.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	367003.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.48925574407171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.87423911913584	58.857970304769424
2	5.538241801782039	7.253906674588251
3	1.4837772094324093	2.9151439540723234
4	0.6964213530447887	1.824324643807305
5	0.3995428914032994	1.3082883297918275
6	0.28574784542034376	1.1228048236230221
7	0.21879904447374982	1.0030290606069947
8	0.1649958138209363	0.8644362438416434
9	0.13353943265100132	0.7870858251119699
>10	1.0469695247309267	14.010382244125369
>50	0.11296023954452249	5.028779841841242
>100	0.04351061078741096	4.2106510084596005
>500	4.1837125757125925E-4	0.1441178052088593
>1k	8.367425151425185E-4	0.6690792401521566
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1388	0.378198543336158	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1054	0.28719111287918625	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	526	0.1433230791028956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.724773366975202E-4	0.0	0.0
2	0.0	0.0	2.724773366975202E-4	0.0	0.0
3	0.0	0.0	8.174320100925606E-4	0.0	0.0
4	0.0	0.0	8.174320100925606E-4	0.0	0.0
5	0.0	0.0	8.174320100925606E-4	0.0	0.0
6	0.0	0.0	0.0019073413568826414	0.0	0.0
7	0.0	0.0	0.002997250703672722	0.0	0.0
8	0.0	0.0	0.0032697280403702423	0.0	0.0
9	0.0	0.0	0.003814682713765283	2.724773366975202E-4	0.0
10	0.0	0.0	0.004087160050462803	2.724773366975202E-4	0.0
11	0.0	0.0	0.0065394560807404845	2.724773366975202E-4	0.0
12	0.0	0.0	0.007084410754135525	2.724773366975202E-4	0.0
13	0.0	0.0	0.007356888090833045	2.724773366975202E-4	0.0
14	0.0	0.0	0.007356888090833045	2.724773366975202E-4	0.0
15	0.0	0.0	0.008174320100925605	2.724773366975202E-4	0.0
16	0.0	0.0	0.008991752111018166	2.724773366975202E-4	0.0
17	0.0	0.0	0.009264229447715686	2.724773366975202E-4	0.0
18	0.0	0.0	0.009809184121110726	5.449546733950404E-4	0.0
19	0.0	0.0	0.009809184121110726	8.174320100925606E-4	0.0
20	0.0	0.0	0.009809184121110726	8.174320100925606E-4	0.0
21	0.0	0.0	0.009809184121110726	8.174320100925606E-4	0.0
22	0.0	0.0	0.010354138794505767	0.0010899093467900808	0.0
23	0.0	0.0	0.010354138794505767	0.0013623866834876009	0.0
24	0.0	0.0	0.010626616131203288	0.0013623866834876009	0.0
25	0.0	0.0	0.010626616131203288	0.0013623866834876009	0.0
26	0.0	0.0	0.010626616131203288	0.0013623866834876009	0.0
27	0.0	0.0	0.010626616131203288	0.0016348640201851211	0.0
28	0.0	0.0	0.010626616131203288	0.008991752111018166	0.0
29	0.0	0.0	0.010626616131203288	0.020163322915616493	0.0
30	0.0	0.0	0.010626616131203288	0.034877099097282586	0.0
31	0.0	0.0	0.010626616131203288	0.09482211317073702	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	245	0.0	30.20408	1
CGGCAAG	45	3.99849E-6	28.777777	18
GTATCAA	660	0.0	23.265152	1
GACAGCG	40	0.0019286971	23.125	7
GCCCCGT	50	2.6972484E-4	22.2	14
CGGTTTA	50	2.6972484E-4	22.2	15
TAGAACA	100	1.2825694E-8	20.35	4
GGTACCG	55	5.133929E-4	20.181818	6
TAGGACC	55	5.133929E-4	20.181818	4
TAGTATG	55	5.133929E-4	20.181818	23
TGGCGGC	65	6.88578E-5	19.923077	15
CTAGAAC	90	2.1441047E-6	18.5	3
CGGCCGT	50	0.007025983	18.5	13
GTCCTAC	60	9.220494E-4	18.5	1
GTCTAAG	60	9.220494E-4	18.5	1
CCATCGG	50	0.007025983	18.5	1
ATATAGG	60	9.220494E-4	18.5	1
GTACTAC	50	0.007025983	18.5	1
CGCAAGG	50	0.007025983	18.5	12
GCCGTTA	50	0.007025983	18.5	15
>>END_MODULE