FastQCFastQC Report
Fri 10 Feb 2017
ERR1633126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327990
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10520.32074148602091523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.2460440867099607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4100.1250038110918016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTA200.001840018737.036
TTAGAAC401.5954538E-632.3753
CTAGACT250.00549068829.64
ATCGCCG250.00549068829.632
GTATCAA4100.025.7195111
GTATTAG1300.022.769231
GGTATCA1400.022.4642871
GGGAGCG450.003820068220.5555535
TAGGGGT450.003820068220.5555534
GATAGAG450.003820068220.5555537
TTATGCG555.1323284E-420.1818184
TATGCGG555.1323284E-420.1818185
CGGGGAT555.1323284E-420.18181817
CTTATGC555.1323284E-420.1818183
AGGTCGC555.1323284E-420.18181835
TAATACT759.232701E-619.7333344
TTAGGAC500.00702442818.53
TAGAACT500.00702442818.54
CATAGGC500.00702442818.53
ATGCGGA609.217642E-418.56
Produced by FastQC (version 0.11.5)