FastQCFastQC Report
Fri 10 Feb 2017
ERR1633125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255536
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13990.547476676476113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.4422077515496838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6190.24223592761880908No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3370.13187965687809156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.11309561079456516No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2690.10526892492642916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGT200.001839258437.022
CGGTCTG200.001839258437.025
TCCGGTC200.001839258437.023
ACTATTC250.00548844529.63
CCGCTCT250.00548844529.628
TAGCCAG250.00548844529.65
CCGGTCT250.00548844529.624
ACTCCGG250.00548844529.621
GGTATCA2400.028.5208341
AGGACTG509.0550075E-625.9000025
TCTGCAC400.001927101323.1253
ATACTCT400.001927101323.1256
GTATCAA5750.020.9130441
TTAGCAC450.003817791120.5555553
CTGCACT450.003817791120.5555554
GTTATAC450.003817791120.5555553
CTGCTCG609.2100404E-418.59
AGGTACT500.007020282618.54
TAGGTAC609.2100404E-418.53
CTCCATA500.007020282618.532
Produced by FastQC (version 0.11.5)