##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633125.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255536
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.744634806837393	33.0	33.0	33.0	27.0	33.0
2	30.5399513180139	33.0	33.0	33.0	27.0	33.0
3	30.98048024544487	33.0	33.0	33.0	27.0	33.0
4	31.157058105315883	33.0	33.0	33.0	27.0	33.0
5	31.264287615052282	33.0	33.0	33.0	27.0	33.0
6	33.667522384321586	37.0	33.0	37.0	27.0	37.0
7	33.929532433786235	37.0	33.0	37.0	27.0	37.0
8	34.09039822177697	37.0	33.0	37.0	27.0	37.0
9	34.144292780664955	37.0	33.0	37.0	27.0	37.0
10	34.217742314194474	37.0	37.0	37.0	27.0	37.0
11	34.19824212635402	37.0	33.0	37.0	27.0	37.0
12	34.185543328532965	37.0	33.0	37.0	27.0	37.0
13	34.23629938638783	37.0	37.0	37.0	27.0	37.0
14	34.20689452758124	37.0	37.0	37.0	27.0	37.0
15	34.20757153590883	37.0	37.0	37.0	27.0	37.0
16	34.24648581804521	37.0	37.0	37.0	27.0	37.0
17	34.21275280195354	37.0	37.0	37.0	27.0	37.0
18	34.23173251518377	37.0	37.0	37.0	27.0	37.0
19	34.208792498904266	37.0	37.0	37.0	27.0	37.0
20	34.23277737774717	37.0	37.0	37.0	27.0	37.0
21	34.217131832696765	37.0	37.0	37.0	27.0	37.0
22	34.2267390895999	37.0	37.0	37.0	27.0	37.0
23	34.1869716987039	37.0	37.0	37.0	27.0	37.0
24	34.173861999874774	37.0	33.0	37.0	27.0	37.0
25	34.14018768392712	37.0	33.0	37.0	27.0	37.0
26	34.10287395905078	37.0	33.0	37.0	27.0	37.0
27	34.04223279694446	37.0	33.0	37.0	27.0	37.0
28	33.99567575605786	37.0	33.0	37.0	27.0	37.0
29	34.04089052031808	37.0	33.0	37.0	27.0	37.0
30	33.979533216454826	37.0	33.0	37.0	27.0	37.0
31	33.98835389142821	37.0	33.0	37.0	27.0	37.0
32	34.01308230542859	37.0	33.0	37.0	27.0	37.0
33	33.97196872456327	37.0	33.0	37.0	27.0	37.0
34	33.87715233861374	37.0	33.0	37.0	27.0	37.0
35	33.87031572850792	37.0	33.0	37.0	27.0	37.0
36	33.82495617055914	37.0	33.0	37.0	27.0	37.0
37	33.863095610794566	37.0	33.0	37.0	27.0	37.0
38	33.72518157911214	37.0	33.0	37.0	27.0	37.0
39	33.75431641725628	37.0	33.0	37.0	27.0	37.0
40	33.775057134806836	37.0	33.0	37.0	27.0	37.0
41	33.7966822678605	37.0	33.0	37.0	27.0	37.0
42	33.655277534280884	37.0	33.0	37.0	27.0	37.0
43	33.37077750297414	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	21.0
17	15.0
18	18.0
19	32.0
20	85.0
21	192.0
22	538.0
23	1084.0
24	2002.0
25	3157.0
26	4635.0
27	6208.0
28	8103.0
29	9884.0
30	12095.0
31	14783.0
32	18160.0
33	22534.0
34	30242.0
35	48509.0
36	73232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.44596456076639	21.384853797507983	13.771053784985284	24.39812785674034
2	16.29555131175255	24.376995804896374	36.505619560453326	22.821833322897753
3	18.54337549308121	27.555412935946404	30.221964811220335	23.67924675975205
4	13.591040010018157	19.228993175129926	38.535079206060985	28.64488760879093
5	14.920793939014466	37.007701458894246	34.41002441925991	13.661480182831381
6	28.84212009266796	40.5160133992862	17.529037004570785	13.112829503475048
7	25.87776282011145	32.951521507732764	23.314523198296914	17.85619247385887
8	24.35351574729197	35.85365662763759	20.852247824181326	18.94057980088911
9	26.152088159789617	15.360653684803708	20.316902510800826	38.17035564460585
10	14.924707281948532	28.644496274497527	33.70131801390019	22.729478429653746
11	34.00773276563772	24.011098240561015	24.10619247385887	17.874976519942397
12	22.538898628764635	25.945854987164235	30.938106568154783	20.577139815916347
13	28.444915784860058	22.33149145325903	26.292968505416063	22.930624256464842
14	21.9440705027863	21.346503036754115	28.473483188278752	28.23594327218083
15	23.858477866132365	28.804943334794313	24.704933942771273	22.631644856302046
16	22.461805772963498	28.581491453259034	25.77914657817294	23.177556195604534
17	22.227396531212822	27.84656565024106	26.965280821488946	22.960756997057167
18	22.969757685805522	26.95001878404608	28.216376557510486	21.86384697263791
19	24.21224406737211	26.9163640348131	27.863784359150962	21.007607538663827
20	23.894871955419198	26.353233986600717	27.885699079581745	21.866194978398347
21	22.967801014338487	26.975846847410935	28.15650241061925	21.89984972763133
22	22.67430029428339	26.972716173063677	27.9522259094609	22.400757623192035
23	22.682126980151523	26.838879844718555	28.183504476864318	22.295488698265604
24	22.50524387953165	27.19029804019786	27.758124099931123	22.546333980339366
25	22.91614488760879	26.815399787114146	28.150241061924742	22.118214263352325
26	22.980715046020915	27.215343434975892	27.758124099931123	22.045817419072065
27	22.909100870327467	27.06781040636153	27.873567716486132	22.14952100682487
28	22.52246258844155	27.028285642727447	28.098976269488446	22.35027549934256
29	23.246039696950724	26.87331726253835	27.420793939014466	22.459849101496463
30	23.122378060234176	26.806007764072383	27.853609667522385	22.21800450817106
31	23.045676538726443	26.713652870828376	27.83560829002567	22.40506230041951
32	22.17652307306994	27.120249201678043	28.066495523135682	22.636732202116335
33	22.355754179450255	26.905798008891114	28.385432972262226	22.35301483939641
34	22.48724250203494	26.933974078016405	27.984706655813664	22.594076764134996
35	22.438717049652496	27.32843904577046	28.050842151399415	22.182001753177634
36	22.94197295097364	27.102639158474734	27.637593137561833	22.317794752989794
37	23.16072882098804	26.67530211007451	27.612547742783793	22.551421326153655
38	22.817919979963683	26.938670089537286	27.60472105691566	22.63868887358337
39	22.533811282950346	26.737524262726193	28.074713543297225	22.653950911026236
40	22.62068749608666	27.08150710663077	28.024231419447748	22.273573977834825
41	22.63242752488886	26.79426773527018	28.018361405046647	22.554943334794313
42	22.47198046459207	27.288522947842964	27.74207939390145	22.497417193663516
43	22.68721432596581	26.503897689562333	27.930702523323525	22.87818546114833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	8.5
2	16.0
3	66.5
4	117.0
5	117.0
6	166.0
7	215.0
8	223.5
9	232.0
10	316.5
11	401.0
12	401.0
13	607.5
14	814.0
15	1489.5
16	2165.0
17	2348.0
18	2531.0
19	2531.0
20	2391.5
21	2252.0
22	2471.5
23	2691.0
24	3345.5
25	4000.0
26	4000.0
27	5009.5
28	6019.0
29	7124.5
30	8230.0
31	9370.5
32	10511.0
33	10511.0
34	11981.5
35	13452.0
36	14443.5
37	15435.0
38	16124.0
39	16813.0
40	16813.0
41	17737.5
42	18662.0
43	19078.5
44	19495.0
45	19495.0
46	19495.0
47	19495.0
48	19419.5
49	19344.0
50	19075.5
51	18807.0
52	17874.5
53	16942.0
54	16942.0
55	15829.5
56	14717.0
57	13343.5
58	11970.0
59	10738.0
60	9506.0
61	9506.0
62	8225.5
63	6945.0
64	5792.0
65	4639.0
66	4066.0
67	3493.0
68	3493.0
69	2864.0
70	2235.0
71	1787.5
72	1340.0
73	1136.0
74	932.0
75	932.0
76	700.0
77	468.0
78	376.5
79	285.0
80	217.0
81	149.0
82	149.0
83	125.5
84	102.0
85	81.5
86	61.0
87	48.0
88	35.0
89	35.0
90	25.0
91	15.0
92	9.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	255536.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.53724768287978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.03500147439092	68.76533454182395
2	5.371502657843226	8.114970533895017
3	1.3679187367026124	3.099864492730718
4	0.609631286269424	1.8419947786466426
5	0.35388941163436427	1.3365916069486787
6	0.23746033444736198	1.076226005880591
7	0.17940626149053873	0.9486298647049227
8	0.11143209608554172	0.6733819073468819
9	0.09091654757698187	0.6180827195515324
>10	0.5765003783737674	7.994591692225378
>50	0.050273899007563204	2.6023018440085885
>100	0.014512053321770819	1.6955873757185491
>500	5.182876186346721E-4	0.24233862516042068
>1k	0.0010365752372693442	0.9901040113581646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1399	0.547476676476113	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1130	0.4422077515496838	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.24223592761880908	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	337	0.13187965687809156	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	289	0.11309561079456516	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	269	0.10526892492642916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.9133429340679985E-4	0.0	0.0
2	0.0	0.0	3.9133429340679985E-4	0.0	0.0
3	0.0	0.0	3.9133429340679985E-4	0.0	0.0
4	0.0	0.0	7.826685868135997E-4	0.0	0.0
5	0.0	0.0	0.002348005760440799	0.0	0.0
6	0.0	0.0	0.003130674347254399	0.0	0.0
7	0.0	0.0	0.0070440172813223964	3.9133429340679985E-4	0.0
8	0.0	0.0	0.009783357335169995	3.9133429340679985E-4	0.0
9	0.0	0.0	0.012131363095610794	7.826685868135997E-4	0.0
10	3.9133429340679985E-4	0.0	0.012522697389017595	7.826685868135997E-4	0.0
11	3.9133429340679985E-4	0.0	0.01682737461649239	7.826685868135997E-4	0.0
12	3.9133429340679985E-4	0.0	0.017610043203305992	7.826685868135997E-4	0.0
13	3.9133429340679985E-4	0.0	0.019175380376933192	7.826685868135997E-4	0.0
14	3.9133429340679985E-4	0.0	0.02269738901759439	7.826685868135997E-4	0.0
15	3.9133429340679985E-4	0.0	0.02426272619122159	7.826685868135997E-4	0.0
16	3.9133429340679985E-4	0.0	0.02543672907144199	7.826685868135997E-4	0.0
17	3.9133429340679985E-4	0.0	0.02582806336484879	7.826685868135997E-4	0.0
18	3.9133429340679985E-4	0.0	0.026610731951662386	0.0011740028802203996	0.0
19	3.9133429340679985E-4	0.0	0.026610731951662386	0.0011740028802203996	0.0
20	3.9133429340679985E-4	0.0	0.02700206624506919	0.0011740028802203996	0.0
21	3.9133429340679985E-4	0.0	0.02700206624506919	0.0011740028802203996	0.0
22	3.9133429340679985E-4	0.0	0.028567403418696388	0.0015653371736271994	0.0
23	3.9133429340679985E-4	0.0	0.028567403418696388	0.0015653371736271994	0.0
24	3.9133429340679985E-4	0.0	0.028567403418696388	0.0015653371736271994	0.0
25	3.9133429340679985E-4	0.0	0.028567403418696388	0.0019566714670339993	0.0
26	3.9133429340679985E-4	0.0	0.028567403418696388	0.0019566714670339993	0.0
27	3.9133429340679985E-4	0.0	0.028567403418696388	0.002348005760440799	0.0
28	3.9133429340679985E-4	0.0	0.028567403418696388	0.008609354454949597	0.0
29	3.9133429340679985E-4	0.0	0.028567403418696388	0.028567403418696388	0.0
30	3.9133429340679985E-4	0.0	0.028567403418696388	0.05361279819673158	0.0
31	3.9133429340679985E-4	0.0	0.028567403418696388	0.10800826498027676	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGGT	20	0.0018392584	37.0	22
CGGTCTG	20	0.0018392584	37.0	25
TCCGGTC	20	0.0018392584	37.0	23
ACTATTC	25	0.005488445	29.6	3
CCGCTCT	25	0.005488445	29.6	28
TAGCCAG	25	0.005488445	29.6	5
CCGGTCT	25	0.005488445	29.6	24
ACTCCGG	25	0.005488445	29.6	21
GGTATCA	240	0.0	28.520834	1
AGGACTG	50	9.0550075E-6	25.900002	5
TCTGCAC	40	0.0019271013	23.125	3
ATACTCT	40	0.0019271013	23.125	6
GTATCAA	575	0.0	20.913044	1
TTAGCAC	45	0.0038177911	20.555555	3
CTGCACT	45	0.0038177911	20.555555	4
GTTATAC	45	0.0038177911	20.555555	3
CTGCTCG	60	9.2100404E-4	18.5	9
AGGTACT	50	0.0070202826	18.5	4
TAGGTAC	60	9.2100404E-4	18.5	3
CTCCATA	50	0.0070202826	18.5	32
>>END_MODULE