FastQCFastQC Report
Fri 10 Feb 2017
ERR1633124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420658
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27820.661344845456404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21720.5163339339796225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10270.2441413214535323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.14453546586538232No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4350.10340942047934426No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4280.10174536083944678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3800.026.7763161
CGAGTCG952.8012437E-1023.36842221
GTATCAA11000.022.8727281
CCGTCCG903.810783E-922.6111128
CCGCCGC903.810783E-922.6111132
TCCGCCG957.1122486E-921.42105331
CGTCCGC1059.767973E-1021.14285729
ACGGTGG705.089243E-621.1428578
GGCGAGT1151.3460522E-1020.91304419
GCCGTCC1001.2840246E-820.3527
CGGGATT555.135646E-420.18181817
ACACTCT555.135646E-420.1818186
TGTCCGG1202.3646862E-1020.04166814
TCGCCGT1052.2491804E-819.3809525
GAGTCGC1052.2491804E-819.3809522
ACCGCAA801.6127033E-518.58
ACATTGC500.007027649318.58
TAGTTCC500.007027649318.54
GTCCGCC1103.8342478E-818.530
CGCCGTC1103.8342478E-818.526
Produced by FastQC (version 0.11.5)