##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633124.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420658
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.766855735538133	33.0	33.0	33.0	27.0	33.0
2	31.286498770973093	33.0	33.0	33.0	27.0	33.0
3	31.71409791326921	33.0	33.0	33.0	27.0	33.0
4	31.839879426992947	33.0	33.0	33.0	27.0	33.0
5	31.954977677828545	33.0	33.0	33.0	33.0	33.0
6	34.855352804415936	37.0	37.0	37.0	27.0	37.0
7	35.022624079418435	37.0	37.0	37.0	33.0	37.0
8	35.19794702584998	37.0	37.0	37.0	33.0	37.0
9	35.283608061655784	37.0	37.0	37.0	33.0	37.0
10	35.304946060695386	37.0	37.0	37.0	33.0	37.0
11	35.31856282300586	37.0	37.0	37.0	33.0	37.0
12	35.2976978923496	37.0	37.0	37.0	33.0	37.0
13	35.31359441636674	37.0	37.0	37.0	33.0	37.0
14	35.28401219042548	37.0	37.0	37.0	33.0	37.0
15	35.30355062782593	37.0	37.0	37.0	33.0	37.0
16	35.309070551374276	37.0	37.0	37.0	33.0	37.0
17	35.29237765595805	37.0	37.0	37.0	33.0	37.0
18	35.288543187102114	37.0	37.0	37.0	33.0	37.0
19	35.28629670658825	37.0	37.0	37.0	33.0	37.0
20	35.29241093715085	37.0	37.0	37.0	33.0	37.0
21	35.2898815664982	37.0	37.0	37.0	33.0	37.0
22	35.2960124376572	37.0	37.0	37.0	33.0	37.0
23	35.28328000418392	37.0	37.0	37.0	33.0	37.0
24	35.2689857318772	37.0	37.0	37.0	33.0	37.0
25	35.26477090653215	37.0	37.0	37.0	33.0	37.0
26	35.24853443890286	37.0	37.0	37.0	33.0	37.0
27	35.185571176585256	37.0	37.0	37.0	33.0	37.0
28	35.14069624255333	37.0	37.0	37.0	33.0	37.0
29	35.1786035211502	37.0	37.0	37.0	33.0	37.0
30	35.143622610291494	37.0	37.0	37.0	33.0	37.0
31	35.1293212063006	37.0	37.0	37.0	33.0	37.0
32	35.15133433810839	37.0	37.0	37.0	33.0	37.0
33	35.124816359132595	37.0	37.0	37.0	33.0	37.0
34	35.05571271674377	37.0	37.0	37.0	33.0	37.0
35	35.04410946659757	37.0	37.0	37.0	27.0	37.0
36	35.00386537282068	37.0	37.0	37.0	27.0	37.0
37	35.02331585278302	37.0	37.0	37.0	27.0	37.0
38	34.86938320440833	37.0	37.0	37.0	27.0	37.0
39	34.92632732528563	37.0	37.0	37.0	27.0	37.0
40	34.95430729951647	37.0	37.0	37.0	27.0	37.0
41	34.945628039880376	37.0	37.0	37.0	27.0	37.0
42	34.842639388767125	37.0	37.0	37.0	27.0	37.0
43	34.62589562067048	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	26.0
17	35.0
18	14.0
19	15.0
20	24.0
21	86.0
22	253.0
23	511.0
24	1119.0
25	2001.0
26	3311.0
27	5142.0
28	7345.0
29	9785.0
30	12707.0
31	16368.0
32	20797.0
33	27226.0
34	38871.0
35	70483.0
36	204530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.634910069462606	21.04393592894941	13.974297410247754	24.34685659134023
2	15.464106233567412	23.676716002072943	38.36774767150512	22.49143009285453
3	17.645926144278725	27.540187040303525	31.070132982137512	23.74375383328024
4	12.364914015661178	18.29847524592424	39.768410442687404	29.56820029572717
5	13.766052232454868	37.226678204146836	35.270457236044486	13.736812327353812
6	28.647499869252457	40.56121599969572	17.60194742522429	13.189336705827536
7	25.37857356807668	33.19275991422961	23.91776692705238	17.51089959064133
8	24.25129202344898	35.73544304399298	21.09528405498053	18.91798087757751
9	26.006874943540836	15.201897978880705	20.69234389931964	38.09888317825882
10	14.669636616919208	28.46469103166943	34.20118005600749	22.664492295403868
11	33.51083302825573	23.949146337404734	24.614057024946632	17.925963609392902
12	22.527563959320872	26.10838258157458	30.917277218072638	20.44677624103191
13	27.82735618958869	22.73604685991946	26.669883848637134	22.766713101854712
14	21.77279405122451	21.27524021889516	28.73926087225252	28.212704857627813
15	23.491292213627222	28.959867635941787	25.20051918660765	22.348320963823344
16	21.9791374465718	28.85931088913084	26.086749806255916	23.07480185804145
17	21.67342591844206	27.756277070684497	27.295332550432892	23.274964460440547
18	22.65046664986759	26.998654488919737	28.853843264599742	21.497035596612925
19	23.985993372288178	26.698410585321092	28.428557165203088	20.887038877187646
20	23.73376947544086	26.287863299877813	28.369364186583873	21.60900303809746
21	22.606250208007456	27.175520256360276	28.413342905638313	21.804886629993963
22	22.265831150245567	27.053330734230656	28.341075172705622	22.339762942818155
23	22.269634715136764	27.08352153055451	28.7501961213147	21.896647632994025
24	22.401570872300063	27.24422214720747	27.932429669707933	22.42177731078453
25	22.562033766147323	27.176708870388772	28.806298703459817	21.45495866000409
26	22.553475745142133	27.345967508046915	28.323483685083843	21.777073061727105
27	22.567739113484116	27.070209053435335	28.401932210964727	21.96011962211583
28	22.189522129615984	27.055707962287656	28.37388091989217	22.380888988204195
29	22.7771729053055	27.119893119826553	28.09384345477799	22.009090520089956
30	22.82138934716563	27.016483699347216	28.482044796485507	21.68008215700165
31	22.572731292403805	26.995088646834247	28.24765011006566	22.18452995069629
32	21.664154729019774	27.041206871139977	28.60518520983792	22.68945319000233
33	21.635152546724417	27.15032163895611	28.94607971321121	22.268446101108264
34	22.08563726352524	26.778285448036172	28.58997095027314	22.546106338165444
35	22.033813691882717	27.37568285875937	28.525785792734244	22.06471765662367
36	22.82923419975372	27.02195132387831	28.25620813107085	21.892606345297132
37	22.448402265022892	26.967988246984486	28.279742688835114	22.30386679915751
38	22.402046317911463	27.21949897541471	27.970465318619876	22.407989388053952
39	21.890704562851532	26.962045176841997	28.50225123496998	22.644999025336496
40	22.01741081828944	27.528300900018543	28.62990838163068	21.824379900061334
41	22.225893718888027	26.818222879393712	28.36104388838439	22.594839513333874
42	21.85409525077379	27.397077911272337	28.422851817866295	22.325975020087576
43	22.259888080103078	26.689852564315903	28.502488957775675	22.54777039780534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	30.0
2	50.0
3	198.0
4	346.0
5	346.0
6	428.5
7	511.0
8	475.0
9	439.0
10	607.0
11	775.0
12	775.0
13	1253.5
14	1732.0
15	2844.0
16	3956.0
17	4385.5
18	4815.0
19	4815.0
20	4403.5
21	3992.0
22	4383.5
23	4775.0
24	5967.5
25	7160.0
26	7160.0
27	8701.0
28	10242.0
29	12099.0
30	13956.0
31	16008.5
32	18061.0
33	18061.0
34	20187.0
35	22313.0
36	23954.5
37	25596.0
38	27369.0
39	29142.0
40	29142.0
41	30352.5
42	31563.0
43	31983.5
44	32404.0
45	32364.0
46	32324.0
47	32324.0
48	31722.0
49	31120.0
50	30575.0
51	30030.0
52	28871.0
53	27712.0
54	27712.0
55	25507.5
56	23303.0
57	20691.0
58	18079.0
59	16649.5
60	15220.0
61	15220.0
62	12963.0
63	10706.0
64	8887.5
65	7069.0
66	6050.5
67	5032.0
68	5032.0
69	4135.0
70	3238.0
71	2619.0
72	2000.0
73	1700.0
74	1400.0
75	1400.0
76	1022.5
77	645.0
78	518.5
79	392.0
80	311.5
81	231.0
82	231.0
83	178.5
84	126.0
85	118.5
86	111.0
87	78.5
88	46.0
89	46.0
90	38.5
91	31.0
92	17.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	420658.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.15644414277358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.55743077960157	61.03916028500225
2	6.008727018862468	8.190669348605484
3	1.554432846156154	3.1783384635820333
4	0.750460115206891	2.0459477169391156
5	0.4419783090821465	1.5061834967637415
6	0.28138739125835854	1.1507018408868652
7	0.18705671474640498	0.8924384371100855
8	0.1495283481269717	0.8153056405501717
9	0.13049292183188302	0.8004540184075848
>10	0.8228580915710425	10.920584920877594
>50	0.07674786219069611	3.600695677269454
>100	0.03749781392887238	4.285723549784853
>500	3.5044685914834E-4	0.14522208762577
>1k	0.0010513405774450202	1.4285745165949513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2782	0.661344845456404	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2172	0.5163339339796225	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.2441413214535323	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.14453546586538232	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	435	0.10340942047934426	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	428	0.10174536083944678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.001426336834197852	0.0	0.0
2	0.0	0.0	0.001426336834197852	0.0	0.0
3	0.0	0.0	0.001664059639897494	0.0	0.0
4	0.0	0.0	0.003090396474095346	0.0	0.0
5	0.0	0.0	0.00475445611399284	0.0	0.0
6	0.0	0.0	0.007844852588088185	0.0	0.0
7	0.0	0.0	0.014501091147678162	0.0	0.0
8	0.0	0.0	0.017591487621773507	0.0	0.0
9	0.0	0.0	0.019493270067370644	2.3772280569964198E-4	0.0
10	4.7544561139928397E-4	0.0	0.021157329707268135	4.7544561139928397E-4	0.0
11	4.7544561139928397E-4	0.0	0.029477627906755607	4.7544561139928397E-4	0.0
12	4.7544561139928397E-4	0.0	0.03304346999225024	4.7544561139928397E-4	0.0
13	4.7544561139928397E-4	0.0	0.03494525243784737	4.7544561139928397E-4	0.0
14	4.7544561139928397E-4	0.0	0.03589614366064594	4.7544561139928397E-4	0.0
15	4.7544561139928397E-4	0.0	0.04112604538603806	4.7544561139928397E-4	2.3772280569964198E-4
16	9.508912227985679E-4	0.0	0.04421644186013341	4.7544561139928397E-4	2.3772280569964198E-4
17	9.508912227985679E-4	0.0	0.04564277869433126	7.13168417098926E-4	2.3772280569964198E-4
18	9.508912227985679E-4	0.0	0.04659366991712983	7.13168417098926E-4	2.3772280569964198E-4
19	9.508912227985679E-4	0.0	0.0475445611399284	7.13168417098926E-4	2.3772280569964198E-4
20	9.508912227985679E-4	0.0	0.04849545236272697	7.13168417098926E-4	2.3772280569964198E-4
21	9.508912227985679E-4	0.0	0.04992178919692482	9.508912227985679E-4	2.3772280569964198E-4
22	9.508912227985679E-4	0.0	0.051110403225423026	0.00237722805699642	2.3772280569964198E-4
23	9.508912227985679E-4	0.0	0.05158584883682231	0.002852673668395704	2.3772280569964198E-4
24	9.508912227985679E-4	0.0	0.05182357164252195	0.003090396474095346	2.3772280569964198E-4
25	9.508912227985679E-4	0.0	0.05182357164252195	0.0035658420854946297	2.3772280569964198E-4
26	9.508912227985679E-4	0.0	0.05182357164252195	0.004041287696893913	2.3772280569964198E-4
27	9.508912227985679E-4	0.0	0.05182357164252195	0.00475445611399284	2.3772280569964198E-4
28	9.508912227985679E-4	0.0	0.05182357164252195	0.014738813953377804	2.3772280569964198E-4
29	9.508912227985679E-4	0.0	0.05206129444822159	0.03732248049484379	2.3772280569964198E-4
30	9.508912227985679E-4	0.0	0.05206129444822159	0.06798872243009761	2.3772280569964198E-4
31	9.508912227985679E-4	0.0	0.05206129444822159	0.14691269392237874	2.3772280569964198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	380	0.0	26.776316	1
CGAGTCG	95	2.8012437E-10	23.368422	21
GTATCAA	1100	0.0	22.872728	1
CCGTCCG	90	3.810783E-9	22.61111	28
CCGCCGC	90	3.810783E-9	22.61111	32
TCCGCCG	95	7.1122486E-9	21.421053	31
CGTCCGC	105	9.767973E-10	21.142857	29
ACGGTGG	70	5.089243E-6	21.142857	8
GGCGAGT	115	1.3460522E-10	20.913044	19
GCCGTCC	100	1.2840246E-8	20.35	27
CGGGATT	55	5.135646E-4	20.181818	17
ACACTCT	55	5.135646E-4	20.181818	6
TGTCCGG	120	2.3646862E-10	20.041668	14
TCGCCGT	105	2.2491804E-8	19.38095	25
GAGTCGC	105	2.2491804E-8	19.38095	22
ACCGCAA	80	1.6127033E-5	18.5	8
ACATTGC	50	0.0070276493	18.5	8
TAGTTCC	50	0.0070276493	18.5	4
GTCCGCC	110	3.8342478E-8	18.5	30
CGCCGTC	110	3.8342478E-8	18.5	26
>>END_MODULE