FastQCFastQC Report
Fri 10 Feb 2017
ERR1633123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459509
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19080.41522581712218914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.32143004816010134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6580.14319632477274655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA250.00549295329.610
GGACCGT250.00549295329.66
GCCGGCA706.5701897E-926.42857215
TTCGCCG706.5701897E-926.42857224
GTATCAA8400.024.4464281
AGCTTCG851.9681465E-923.94117721
CGCTCTC851.9681465E-923.94117729
GGTATCA3000.023.4333321
CCTTATA705.090931E-621.1428592
CCGCTCT909.447285E-820.55555528
CGCCGCT909.447285E-820.55555526
TGCTCGC1001.2847522E-820.3510
CCGGCAG951.6706508E-719.47368416
GCCGCTC951.6706508E-719.47368427
GCTTCGC1052.2506356E-819.3809522
TATACTG1254.0927262E-1019.245
CTGCTCG2050.018.9512189
GGCCGTT500.00702861418.514
TATATAC609.2253165E-418.53
CGAGCCT902.1465003E-618.529
Produced by FastQC (version 0.11.5)