##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459509
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6765786959559	33.0	33.0	33.0	27.0	33.0
2	31.203110276403727	33.0	33.0	33.0	27.0	33.0
3	31.64194390098997	33.0	33.0	33.0	27.0	33.0
4	31.786463377213504	33.0	33.0	33.0	27.0	33.0
5	31.89688341251205	33.0	33.0	33.0	33.0	33.0
6	34.75457934447421	37.0	37.0	37.0	27.0	37.0
7	34.94989651998111	37.0	37.0	37.0	27.0	37.0
8	35.10457901803882	37.0	37.0	37.0	33.0	37.0
9	35.196091915501114	37.0	37.0	37.0	33.0	37.0
10	35.208572628610106	37.0	37.0	37.0	33.0	37.0
11	35.24932264656405	37.0	37.0	37.0	33.0	37.0
12	35.20893170754	37.0	37.0	37.0	33.0	37.0
13	35.23718795496933	37.0	37.0	37.0	33.0	37.0
14	35.21641795916946	37.0	37.0	37.0	33.0	37.0
15	35.22689000650695	37.0	37.0	37.0	33.0	37.0
16	35.21224176240291	37.0	37.0	37.0	33.0	37.0
17	35.191297667727945	37.0	37.0	37.0	33.0	37.0
18	35.199216990309225	37.0	37.0	37.0	33.0	37.0
19	35.20994800972342	37.0	37.0	37.0	33.0	37.0
20	35.20848993164443	37.0	37.0	37.0	33.0	37.0
21	35.20330831387416	37.0	37.0	37.0	33.0	37.0
22	35.20699485755448	37.0	37.0	37.0	33.0	37.0
23	35.19273180721161	37.0	37.0	37.0	33.0	37.0
24	35.181504606003365	37.0	37.0	37.0	33.0	37.0
25	35.183600321212424	37.0	37.0	37.0	33.0	37.0
26	35.14885671444955	37.0	37.0	37.0	33.0	37.0
27	35.108600702053714	37.0	37.0	37.0	33.0	37.0
28	35.069696132175864	37.0	37.0	37.0	33.0	37.0
29	35.09769340752847	37.0	37.0	37.0	33.0	37.0
30	35.0507607032724	37.0	37.0	37.0	27.0	37.0
31	35.035825196024454	37.0	37.0	37.0	27.0	37.0
32	35.06627291304414	37.0	37.0	37.0	27.0	37.0
33	35.04365964540412	37.0	37.0	37.0	27.0	37.0
34	34.9746729661443	37.0	37.0	37.0	27.0	37.0
35	34.976252913435864	37.0	37.0	37.0	27.0	37.0
36	34.955504679995386	37.0	37.0	37.0	27.0	37.0
37	34.98091223458083	37.0	37.0	37.0	27.0	37.0
38	34.831211140587016	37.0	37.0	37.0	27.0	37.0
39	34.91060458010616	37.0	37.0	37.0	27.0	37.0
40	34.92581864555428	37.0	37.0	37.0	27.0	37.0
41	34.936913096370255	37.0	37.0	37.0	27.0	37.0
42	34.85314977508602	37.0	37.0	37.0	27.0	37.0
43	34.634052869475894	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	12.0
16	25.0
17	15.0
18	23.0
19	24.0
20	42.0
21	111.0
22	283.0
23	661.0
24	1369.0
25	2471.0
26	4006.0
27	5811.0
28	8491.0
29	11259.0
30	14643.0
31	18495.0
32	23316.0
33	30238.0
34	42982.0
35	77117.0
36	218115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70192313969911	20.645732727759412	13.7710033971043	24.881340735437174
2	15.775316696734992	23.247422792589482	37.79882439734587	23.178436113329663
3	17.64209188503381	26.94724151213578	30.498423317062347	24.91224328576807
4	12.638925461742861	18.095837078272677	39.08454458998627	30.180692869998193
5	14.012347962716726	37.142689261799006	35.236959450195755	13.608003325288514
6	29.58201036323554	39.80727254526027	17.534368206063426	13.076348885440764
7	26.249757893751806	32.41459906117182	23.73207053615925	17.603572508917125
8	24.398651604212322	36.04412535989502	20.77913599080758	18.77808704508508
9	25.818645554276415	15.315695666461377	20.44073130232487	38.424927476937334
10	14.667830227481943	28.218598547580136	34.37473477124496	22.738836453692965
11	34.02697226822543	23.592573812482453	24.273518037731577	18.106935881560535
12	22.708369150549824	25.646940538705444	30.767188455503593	20.87750185524114
13	27.88933405004037	22.391291574267317	26.32483803364026	23.39453634205206
14	21.966490319014426	20.879242843992174	27.8762766344076	29.2779902025858
15	23.576034419347607	28.961783120678813	24.465462047533347	22.996720412440236
16	22.687694909131267	28.543728196836188	25.547921803490247	23.220655090542298
17	22.18933687914709	27.553540844684214	26.86367405208603	23.39344822408266
18	22.897701677225037	27.2397276223099	28.098470323758622	21.76410037670644
19	24.320742357603443	26.615583155063337	27.44168231743013	21.62199216990309
20	23.75078616523289	26.492625824521394	27.70304825367947	22.05353975656625
21	23.001508131505584	26.963345658082865	27.801631741706913	22.23351446870464
22	22.770391874805497	26.782718075162837	27.652559579899417	22.794330470132248
23	22.733178240252094	26.923520540402908	27.926330060999895	22.416971158345103
24	22.845907261881703	27.36616693035392	26.763349575307554	23.024576232456816
25	23.10009161953302	26.900017192263913	27.73460367479201	22.265287513411053
26	22.9353505589662	27.131568696151763	27.621221782380758	22.31185896250128
27	23.09225717015336	26.984455146689186	27.122428505208823	22.800859177948638
28	22.574965887501662	26.903499169765986	27.664528877562788	22.85700606516956
29	23.378214572511094	26.975097332152366	27.103930499729056	22.542757595607483
30	23.283548309173487	26.686310823074194	27.671057585379177	22.359083282373142
31	23.080287872489986	26.78532955828939	27.552235103120942	22.582147466099684
32	22.248095249494572	26.846917035357304	27.848420814391012	23.05656690075711
33	22.207399637439092	27.01426957905068	28.1391659358141	22.63916484769613
34	22.841337166410234	26.555083795964823	27.762894741996348	22.840684295628595
35	22.56974292124855	27.310020043132997	27.731339320883812	22.38889771473464
36	23.466787375220072	26.80905053002226	27.207736954009604	22.51642514074806
37	22.73165487509494	26.686528446668074	27.533519474047296	23.048297204189687
38	23.021311878548627	27.008828989203693	27.176834403678708	22.793024728568973
39	22.542757595607483	26.780106592036283	27.738520899481838	22.938614912874396
40	22.587805679540555	27.21883575729746	27.8336224100072	22.35973615315478
41	22.644605437543117	26.34224792115062	27.83949824704195	23.173648394264312
42	22.275515822323396	27.2003377518177	27.713929433373448	22.810216992485458
43	22.93448006459068	26.33245485942604	27.705006866024384	23.02805820995889
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	18.0
2	28.0
3	129.5
4	231.0
5	231.0
6	276.0
7	321.0
8	300.5
9	280.0
10	403.0
11	526.0
12	526.0
13	806.5
14	1087.0
15	1913.5
16	2740.0
17	3080.0
18	3420.0
19	3420.0
20	3222.0
21	3024.0
22	3463.5
23	3903.0
24	5231.5
25	6560.0
26	6560.0
27	8280.0
28	10000.0
29	11952.0
30	13904.0
31	16361.0
32	18818.0
33	18818.0
34	21274.5
35	23731.0
36	25810.0
37	27889.0
38	30273.0
39	32657.0
40	32657.0
41	34348.5
42	36040.0
43	36679.0
44	37318.0
45	37101.0
46	36884.0
47	36884.0
48	35683.5
49	34483.0
50	34112.0
51	33741.0
52	32192.0
53	30643.0
54	30643.0
55	28697.0
56	26751.0
57	23579.0
58	20407.0
59	18852.0
60	17297.0
61	17297.0
62	14992.0
63	12687.0
64	10585.5
65	8484.0
66	7200.0
67	5916.0
68	5916.0
69	4882.5
70	3849.0
71	3112.5
72	2376.0
73	1954.5
74	1533.0
75	1533.0
76	1195.5
77	858.0
78	665.5
79	473.0
80	377.5
81	282.0
82	282.0
83	219.5
84	157.0
85	134.5
86	112.0
87	85.0
88	58.0
89	58.0
90	41.5
91	25.0
92	16.0
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	459509.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.64960675567556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.81361406779747	58.52742845413361
2	6.926039289665605	9.232355900611422
3	1.9412778733117926	3.8815622057917545
4	0.9236258223802323	2.462371914041197
5	0.5119893615934112	1.7061944806645117
6	0.35372873366937957	1.4145528598348345
7	0.22798992283737207	1.0636807090957363
8	0.17506146285998708	0.9334222126153153
9	0.13194585032362793	0.7914725133401727
>10	0.8775303794163476	11.24219068919905
>50	0.07681835655631332	3.529458148295365
>100	0.0393940288667989	4.330702680637611
>500	3.2828357388999083E-4	0.14397020986508097
>1k	6.565671477799817E-4	0.7406370218743148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1908	0.41522581712218914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1477	0.32143004816010134	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	658	0.14319632477274655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.528707816386622E-4	0.0	0.0
2	0.0	0.0	8.704943755182162E-4	0.0	0.0
3	0.0	0.0	8.704943755182162E-4	0.0	0.0
4	0.0	0.0	8.704943755182162E-4	0.0	0.0
5	2.1762359387955405E-4	0.0	8.704943755182162E-4	0.0	0.0
6	2.1762359387955405E-4	0.0	0.0021762359387955403	0.0	0.0
7	2.1762359387955405E-4	0.0	0.005658213440868405	2.1762359387955405E-4	0.0
8	2.1762359387955405E-4	0.0	0.00696395500414573	2.1762359387955405E-4	0.0
9	4.352471877591081E-4	0.0	0.008269696567423053	6.528707816386622E-4	0.0
10	4.352471877591081E-4	0.0	0.008922567349061716	6.528707816386622E-4	0.0
11	6.528707816386622E-4	0.0	0.014145533602171013	8.704943755182162E-4	0.0
12	6.528707816386622E-4	0.0	0.016757016728725663	8.704943755182162E-4	0.0
13	6.528707816386622E-4	0.0	0.01828038188588254	8.704943755182162E-4	0.0
14	6.528707816386622E-4	0.0	0.018933252667521203	8.704943755182162E-4	0.0
15	6.528707816386622E-4	0.0	0.02045661782467808	8.704943755182162E-4	0.0
16	6.528707816386622E-4	0.0	0.022197606575714512	8.704943755182162E-4	0.0
17	6.528707816386622E-4	0.0	0.02306810095123273	8.704943755182162E-4	0.0
18	6.528707816386622E-4	0.0	0.023503348138991836	8.704943755182162E-4	0.0
19	6.528707816386622E-4	0.0	0.023938595326750946	8.704943755182162E-4	0.0
20	6.528707816386622E-4	0.0	0.0241562189206305	8.704943755182162E-4	0.0
21	6.528707816386622E-4	0.0	0.025026713296148716	8.704943755182162E-4	0.0
22	6.528707816386622E-4	2.1762359387955405E-4	0.025026713296148716	8.704943755182162E-4	0.0
23	6.528707816386622E-4	2.1762359387955405E-4	0.02524433689002827	0.0010881179693977702	0.0
24	6.528707816386622E-4	2.1762359387955405E-4	0.02567958407778738	0.0015233651571568783	0.0
25	6.528707816386622E-4	2.1762359387955405E-4	0.025897207671666932	0.0017409887510364324	0.0
26	6.528707816386622E-4	2.1762359387955405E-4	0.025897207671666932	0.0017409887510364324	0.0
27	6.528707816386622E-4	2.1762359387955405E-4	0.025897207671666932	0.0032643539081933107	0.0
28	6.528707816386622E-4	2.1762359387955405E-4	0.025897207671666932	0.01392791000829146	0.0
29	6.528707816386622E-4	2.1762359387955405E-4	0.025897207671666932	0.032425915488053556	0.0
30	6.528707816386622E-4	2.1762359387955405E-4	0.025897207671666932	0.05788787597196138	0.0
31	6.528707816386622E-4	2.1762359387955405E-4	0.025897207671666932	0.12600406085626178	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAA	25	0.005492953	29.6	10
GGACCGT	25	0.005492953	29.6	6
GCCGGCA	70	6.5701897E-9	26.428572	15
TTCGCCG	70	6.5701897E-9	26.428572	24
GTATCAA	840	0.0	24.446428	1
AGCTTCG	85	1.9681465E-9	23.941177	21
CGCTCTC	85	1.9681465E-9	23.941177	29
GGTATCA	300	0.0	23.433332	1
CCTTATA	70	5.090931E-6	21.142859	2
CCGCTCT	90	9.447285E-8	20.555555	28
CGCCGCT	90	9.447285E-8	20.555555	26
TGCTCGC	100	1.2847522E-8	20.35	10
CCGGCAG	95	1.6706508E-7	19.473684	16
GCCGCTC	95	1.6706508E-7	19.473684	27
GCTTCGC	105	2.2506356E-8	19.38095	22
TATACTG	125	4.0927262E-10	19.24	5
CTGCTCG	205	0.0	18.951218	9
GGCCGTT	50	0.007028614	18.5	14
TATATAC	60	9.2253165E-4	18.5	3
CGAGCCT	90	2.1465003E-6	18.5	29
>>END_MODULE