FastQCFastQC Report
Fri 10 Feb 2017
ERR1633122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400351
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92512.310722341145645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75031.874105472447927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35460.885722778262075No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25760.6434353854492683No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG25160.6284485364093009No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20720.5175458535135419No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.49956163466558096No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT17500.43711643033238334No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17490.4368666495150505No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC14440.36068350022854945No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13850.3459464320059148No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13480.3367045417646016No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13180.3292111172446178No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12210.30498237796333716No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12020.30023654243401415No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11800.2947413644526928No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11730.29299289873136325No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11590.28949596728870414No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11390.2845003509420484No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11330.2830016660380516No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11240.2807536386820565No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10470.2615205157474316No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10460.26127073493009884No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC10330.25802358430477257No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.25152928305412003No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC10070.25152928305412003No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT9920.24778257079412813No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9840.2457843242554658No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT9650.2410384887261428No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9320.23279572175416074No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9240.2307974752154984No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9180.22929879031150166No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8820.2203066808875212No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC8700.21730931107952772No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8500.21231369473287193No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8050.20107355795289636No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC7950.19857574977956843No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7910.19757662651023727No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7800.1948290375195766No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7490.18708583218226008No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7320.18283955828760262No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7290.18209021583560425No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7270.18159065420093867No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7260.18134087338360588No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7250.1810910925662731No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7200.17984218847960914No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6840.17085007905562868No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6830.1706002982382959No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6830.1706002982382959No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.1626073120836466No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6470.16160818881431543No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6460.16135840799698264No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6450.16110862717964985No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6450.16110862717964985No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6430.16060906554498428No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6410.1601095039103187No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.15686235328499243No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6270.15661257246765964No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC6230.15561344919832848No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6200.15486410674633008No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6040.15086761366900545No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6000.1498684903996743No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5920.14787024386101197No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5910.14762046304367918No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC5850.14612177813968244No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5850.14612177813968244No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5780.14437331241835288No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5720.14287462751435617No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5690.14212528506235778No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5600.13987725770636267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.13762923035036756No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5500.13737944953303477No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC5410.13513142217703966No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG5300.13238383318637897No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5270.13163449073438058No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5250.131134929099715No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5210.13013580583038384No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5100.12738821683972312No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5070.12663887438772478No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG5050.1261393127530592No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT4980.12439084703172966No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4970.12414106621439687No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4950.12364150457973128No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4940.12339172376239849No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4910.12264238131040012No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4800.11989479231973943No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4800.11989479231973943No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4750.11864588823307547No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4670.11664764169441316No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4540.11340049106908687No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC4540.11340049106908687No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4490.11215158698242292No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4480.11190180616509013No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC4390.10965377880909502No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4300.1074057514530999No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4300.1074057514530999No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4280.10690618981843432No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC4210.10515772409710479No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG4200.104907943279772No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA4150.10365903919310805No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA4140.10340925837577525No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA4130.10315947755844246No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT4130.10315947755844246No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4100.10241013510644409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCC507.3068804E-933.3000039
TTCCTTG453.999683E-628.77777718
GGTATCA12450.028.0843371
ACTAGTC604.3080945E-827.7499988
GTCCAAA358.8592595E-426.42857212
CGCTCTC3200.026.01562529
GAAATTC509.071777E-625.90000214
TCCTTGT509.071777E-625.90000219
CCGCTCT3250.025.61538528
GCCGCTC3250.025.61538527
GGCAGCT3200.025.437518
GCAGCTT3200.025.437519
GAATCAA951.0913936E-1125.315791
GCTCTCT3350.024.85074630
TTCGCCG3300.024.66666824
TATGCGG601.3337722E-624.6666663
GCCGGCA3400.024.48529415
CTCTCTT3500.024.31428731
CCGGCAG3350.024.29850816
GTATCAA34550.023.9348751
Produced by FastQC (version 0.11.5)