FastQCFastQC Report
Fri 10 Feb 2017
ERR1633121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362215
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11660.32190825890700275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.22997391052275584No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5110.1410764325055561No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4800.13251797965296855No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4580.12644423891887416No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4420.12202697293044187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4270.11788578606628661No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4260.11760970694200959No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3880.10711870021948292No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3810.10518614634954379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2350.030.702131
AGGACCG250.005491436529.5999985
GTACACG400.001928648323.1254
TATTAGC400.001928648323.1252
TAATACA855.1561074E-821.7647064
TCGGCCT855.1561074E-821.76470623
ATTTCGG450.00382082620.55555729
GTATCAA4900.020.0102041
TCGCCAT1153.0304363E-919.30434813
AATTTCG500.00702581118.49999828
GATATAT500.00702581118.4999982
TTCGGTT500.00702581118.49999831
GGGATAA500.00702581118.4999981
TCTCGCC953.5926078E-617.52631612
GGCCGCA1301.3838871E-817.07692333
TTGGCCG1352.2046152E-816.44444531
GTCGCCA1352.2046152E-816.44444512
GCCGCAG1352.2046152E-816.44444534
ACTTTGC904.432445E-516.4444458
ATTACCC803.3745714E-416.18753
Produced by FastQC (version 0.11.5)