FastQCFastQC Report
Fri 10 Feb 2017
ERR1633120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400924
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83922.0931647893366323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66261.6526823038780416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32420.808632059941535No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18160.4529536770061159No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16680.4160389500254412No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16250.40531372529456955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.401073520168411No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14120.3521864493021121No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12310.30704073590007086No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12230.3050453452524668No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10310.25715596970996996No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10140.25291576458381143No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10130.25266634075286093No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8860.22098951422214685No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8780.2189941235745428No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.21649988526503777No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8570.2137562231245822No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8560.2135067992936317No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8440.21051371332222565No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8350.2082688988436711No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8220.20502638904131457No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7940.19804252177470044No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7890.19679540261994793No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7800.19455058814139337No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7600.1895621115223833No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7540.18806556853668027No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7370.1838253634105217No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7340.1830770919176702No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7060.17609322465105606No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7050.17584380082010553No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7040.17559437698915506No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6770.16885993355349144No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6760.16861050972254094No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6600.16461972842733286No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6580.16412088076543185No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6570.16387145693448135No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6390.15938182797737227No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6360.15863355648452077No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6240.1556404705131147No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6180.15414392752741168No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6070.15140026538695614No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.14441639812034202No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5750.14341870279653998No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5710.14242100747273798No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5590.13942792150133193No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5570.1389290738394309No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5300.1321946304037673No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5280.1316957827418663No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5240.13069808741806427No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5160.12870269677046023No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5120.12770500144665822No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5060.1262084584609552No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5050.12595903463000468No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5030.12546018696810368No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5020.12521076313715318No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4960.12371422015145016No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4910.12246710099669764No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4880.12171882950384612No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4870.1214694056728956No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4870.1214694056728956No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4810.11997286268719258No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4780.11922459119434108No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4760.11872574353244006No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4690.11697977671578655No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4590.11448553840628149No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4580.11423611457533098No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4560.11373726691342999No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4550.11348784308247946No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4500.11224072392772694No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4480.11174187626582595No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4410.10999590944917241No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4370.1089982141253704No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.10874879029441989No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4350.10849936646346939No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4310.10750167113966735No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4300.10725224730871687No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4250.10600512815396435No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4230.10550628049206333No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4230.10550628049206333No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.10525685666111283No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4220.10525685666111283No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4180.10425916133731082No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4150.10351088984445929No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4130.1030120421825583No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4080.10176492302780576No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4080.10176492302780576No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4070.10151549919685525No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4070.10151549919685525No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4060.10126607536590476No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4050.10101665153495426No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4010.10001895621115223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCCC401.5966289E-632.37518
AGGCGTA303.595335E-430.83333220
GAATCAA502.7133137E-729.61
AGAGTTG250.00549212729.628
TAGTTCC250.00549212729.64
GCACTAG250.00549212729.63
GCCCCCG453.999703E-628.77777720
CCGTCAA453.999703E-628.77777724
GGTATCA11200.028.2455351
CGGGTAT405.9306305E-527.75000216
GTAGTCG405.9306305E-527.75000224
CCGCTCT2400.026.97916628
CGGGCCC556.2446634E-726.9090917
CGCTCTC2350.026.76595529
TCCGCGC358.8592834E-426.4285731
CTAGTCC358.8592834E-426.428579
GGCAGCT2600.026.32692318
GCAGCTT2550.026.11764719
GCCGCTC2500.025.90000227
GCTCTCT2500.025.90000230
Produced by FastQC (version 0.11.5)