FastQCFastQC Report
Fri 10 Feb 2017
ERR1633119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561183
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22960.4091357008319924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17530.3123758203651928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9040.16108827245301444No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5800.10335309515790748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.032.309861
GTCTAAT652.9831426E-928.4615361
GTATCAA12400.025.8104821
GACGATC450.003823405420.55555515
TTACTCG555.138587E-420.18181819
ACTCGCA555.138587E-420.18181821
ATTAGAC500.00703050618.4999983
TACTCGC500.00703050618.49999820
CGAACTA1054.7864523E-717.61904729
TAACCGT953.5997364E-617.5263167
ACCGTGC953.5997364E-617.5263169
ATCCCGA752.0648923E-417.26666637
ATCAACG19850.016.7758183
ACGGGGT1553.9835868E-1016.70967716
CATCCCG904.4394365E-516.44444536
TCAACGC20150.016.4342424
CAACGCA20300.016.3128075
AACGCAG20650.016.2154966
TATCAAC20100.016.1069662
AACCGTG1059.325986E-615.8571428
Produced by FastQC (version 0.11.5)