##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561183
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75333358280632	33.0	33.0	33.0	27.0	33.0
2	31.28613482589458	33.0	33.0	33.0	27.0	33.0
3	31.70640236785505	33.0	33.0	33.0	27.0	33.0
4	31.839262771680538	33.0	33.0	33.0	27.0	33.0
5	31.943020369469497	33.0	33.0	33.0	33.0	33.0
6	34.82962776848194	37.0	37.0	37.0	27.0	37.0
7	34.961272882464364	37.0	37.0	37.0	27.0	37.0
8	35.16646441535114	37.0	37.0	37.0	33.0	37.0
9	35.23101555107692	37.0	37.0	37.0	33.0	37.0
10	35.277962803577445	37.0	37.0	37.0	33.0	37.0
11	35.30892061947707	37.0	37.0	37.0	33.0	37.0
12	35.23657345286654	37.0	37.0	37.0	33.0	37.0
13	35.27089202630871	37.0	37.0	37.0	33.0	37.0
14	35.2263521881454	37.0	37.0	37.0	33.0	37.0
15	35.252561107517515	37.0	37.0	37.0	33.0	37.0
16	35.28056088655572	37.0	37.0	37.0	33.0	37.0
17	35.2603927774006	37.0	37.0	37.0	33.0	37.0
18	35.22957929944421	37.0	37.0	37.0	33.0	37.0
19	35.255020198402306	37.0	37.0	37.0	33.0	37.0
20	35.14550333848317	37.0	37.0	37.0	33.0	37.0
21	35.22978422368461	37.0	37.0	37.0	33.0	37.0
22	35.25409714834555	37.0	37.0	37.0	33.0	37.0
23	35.2323733969133	37.0	37.0	37.0	33.0	37.0
24	35.22882731657944	37.0	37.0	37.0	33.0	37.0
25	35.24253229338736	37.0	37.0	37.0	33.0	37.0
26	35.154479020212655	37.0	37.0	37.0	33.0	37.0
27	35.128672821521675	37.0	37.0	37.0	33.0	37.0
28	35.129369563939036	37.0	37.0	37.0	33.0	37.0
29	35.1596734042193	37.0	37.0	37.0	33.0	37.0
30	35.137867683090896	37.0	37.0	37.0	33.0	37.0
31	35.1214897814082	37.0	37.0	37.0	33.0	37.0
32	35.15275587464339	37.0	37.0	37.0	33.0	37.0
33	35.108379975872396	37.0	37.0	37.0	33.0	37.0
34	35.05597817467742	37.0	37.0	37.0	27.0	37.0
35	35.05604232487442	37.0	37.0	37.0	27.0	37.0
36	35.049670071972955	37.0	37.0	37.0	27.0	37.0
37	35.03979272358571	37.0	37.0	37.0	27.0	37.0
38	34.90869645017757	37.0	37.0	37.0	27.0	37.0
39	34.96569924605699	37.0	37.0	37.0	27.0	37.0
40	35.00158058957595	37.0	37.0	37.0	27.0	37.0
41	35.023539558397175	37.0	37.0	37.0	27.0	37.0
42	34.89443194109587	37.0	37.0	37.0	27.0	37.0
43	34.72749887291668	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	16.0
16	27.0
17	43.0
18	15.0
19	29.0
20	41.0
21	115.0
22	297.0
23	752.0
24	1539.0
25	2705.0
26	4450.0
27	6808.0
28	9686.0
29	13185.0
30	17200.0
31	22165.0
32	28101.0
33	36446.0
34	52708.0
35	95957.0
36	268897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48608029822714	20.975688857288976	13.897783788888828	24.640447055595054
2	15.904615784868751	23.484496144751354	38.01184283914517	22.599045231234733
3	17.69511906098367	27.06300796709808	30.73186465021214	24.51000832170611
4	12.712787094405925	18.21740858151441	39.50226574931885	29.567538574760814
5	13.849492946151257	37.22725029090333	35.11385768991577	13.809399073029654
6	28.8877246816101	40.341564159997716	17.61617867968203	13.154532478710154
7	25.98154256276473	32.639976620817094	23.73913678782144	17.63934402859673
8	24.30241115643204	35.80953093732347	20.94503931872491	18.94301858751958
9	25.947685514350933	15.398363813586654	20.556217846941195	38.09773282512122
10	15.019877651318733	28.208267178442682	34.12042061145829	22.651434558780288
11	33.95184814935591	23.874743176468282	24.39934923189049	17.774059442285314
12	22.939219470297566	26.01789434106165	30.376009251883968	20.666876936756815
13	28.281683515003124	22.28595663090293	26.212483271945157	23.219876582148782
14	22.161932916713443	21.12982752506758	27.94703332068149	28.76120623753749
15	23.662156551427966	28.85066012334657	24.53067894073769	22.95650438448777
16	22.630229354773753	28.517791878941452	25.57739632169898	23.27458244458581
17	22.22073726395846	27.73676323053264	26.605937813511815	23.436561691997085
18	23.092823553101216	27.08118385624654	27.82675170131668	21.999240889335564
19	24.64294178547818	26.513989197819605	27.453789583789955	21.389279432912257
20	24.17179422755144	26.422753362094003	27.37057252268868	22.034879887665877
21	23.112246807191237	27.043584713008055	27.626282335708673	22.217886144092034
22	22.755678628896455	26.902988864594974	27.53379913504151	22.807533371467063
23	22.849765584488484	26.78751851000476	28.032388721682587	22.330327183824174
24	22.822679945757447	27.232293209167064	26.78555836509659	23.1594684799789
25	23.08765589834332	27.09846877043674	27.7266061160085	22.08726921521144
26	23.081597268627167	27.11165519981895	27.50528793637726	22.301459595176617
27	22.953296874638042	27.145690443224403	27.18418056142114	22.716832120716415
28	22.574276127395162	26.971772131372475	27.7891525580782	22.66479918315416
29	23.541162152096554	26.884100195479903	27.074056056580474	22.500681595843066
30	23.285095949093254	26.902988864594974	27.42117990031772	22.39073528599405
31	23.45669772605371	26.68630375474667	27.21037522519392	22.6466232940057
32	22.434393058948686	26.650486561424703	27.671900253571476	23.243220126055135
33	22.339058738415098	26.88000171067192	28.10669603320129	22.67424351771169
34	22.939932250264174	26.470687814848276	27.56213213871411	23.02724779617344
35	22.79630708699301	27.16404452736451	27.571754668263292	22.467893717379177
36	23.530292257605808	26.714636758419267	27.041624568099888	22.713446415875037
37	23.039899640580703	26.5702988151815	27.315332075276693	23.074469468961105
38	22.840855834905906	26.973019496314038	27.27755473704656	22.9085699317335
39	22.670145032903704	26.6552978261993	27.82104946158383	22.853507679313164
40	22.662126258279386	27.11807021951841	27.814278051901077	22.405525470301132
41	22.877742198177778	26.264516209507416	27.62592594572537	23.23181564658944
42	22.44062988365649	27.172241496980487	27.578882467929354	22.808246151433668
43	22.912312026558183	26.17720066359815	27.795389382786006	23.11509792705766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	23.5
2	34.0
3	147.5
4	261.0
5	261.0
6	345.5
7	430.0
8	401.5
9	373.0
10	486.5
11	600.0
12	600.0
13	924.5
14	1249.0
15	2208.5
16	3168.0
17	3678.0
18	4188.0
19	4188.0
20	4050.5
21	3913.0
22	4517.5
23	5122.0
24	6746.0
25	8370.0
26	8370.0
27	10418.5
28	12467.0
29	15002.5
30	17538.0
31	20022.0
32	22506.0
33	22506.0
34	25783.0
35	29060.0
36	31474.0
37	33888.0
38	36223.0
39	38558.0
40	38558.0
41	40777.0
42	42996.0
43	44098.0
44	45200.0
45	44768.5
46	44337.0
47	44337.0
48	43542.5
49	42748.0
50	42149.0
51	41550.0
52	39719.0
53	37888.0
54	37888.0
55	35243.5
56	32599.0
57	29041.5
58	25484.0
59	23410.0
60	21336.0
61	21336.0
62	18592.5
63	15849.0
64	13240.0
65	10631.0
66	8958.0
67	7285.0
68	7285.0
69	6027.0
70	4769.0
71	3859.0
72	2949.0
73	2335.0
74	1721.0
75	1721.0
76	1312.0
77	903.0
78	683.0
79	463.0
80	388.5
81	314.0
82	314.0
83	243.5
84	173.0
85	152.0
86	131.0
87	104.5
88	78.0
89	78.0
90	56.5
91	35.0
92	20.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	561183.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.63196203427754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25863923847092	53.16289178673884
2	7.749111579095591	9.551859012844
3	2.0745006992562067	3.8356664500992226
4	0.969799625260488	2.3908261473964396
5	0.5644311195843426	1.7393498666593485
6	0.3891663372892021	1.4391050954896172
7	0.3036667009922172	1.3100902210638652
8	0.20904033509733752	1.030685279708141
9	0.18460683477006404	1.023991328863506
>10	1.1344686835903242	13.618366868087891
>50	0.11254766658844624	4.643713346590133
>100	0.048857896748935016	5.261727568392647
>500	5.816416279634998E-4	0.2659900433129374
>1k	5.816416279634998E-4	0.7257369847534256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2296	0.4091357008319924	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1753	0.3123758203651928	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	904	0.16108827245301444	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	580	0.10335309515790748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.7819499165156464E-4	0.0	0.0
2	0.0	0.0	1.7819499165156464E-4	0.0	0.0
3	0.0	0.0	1.7819499165156464E-4	0.0	0.0
4	0.0	0.0	1.7819499165156464E-4	0.0	0.0
5	0.0	0.0	3.563899833031293E-4	0.0	0.0
6	0.0	0.0	0.0012473649415609524	0.0	0.0
7	0.0	0.0	0.0026729248747734695	1.7819499165156464E-4	0.0
8	0.0	0.0	0.0033857048413797283	1.7819499165156464E-4	0.0
9	0.0	0.0	0.004811264774592245	1.7819499165156464E-4	0.0
10	8.909749582578232E-4	0.0	0.006058629716153198	1.7819499165156464E-4	0.0
11	8.909749582578232E-4	0.0	0.011760869449003267	1.7819499165156464E-4	0.0
12	8.909749582578232E-4	0.0	0.013899209348822042	3.563899833031293E-4	0.0
13	8.909749582578232E-4	0.0	0.01532476928203456	3.563899833031293E-4	0.0
14	8.909749582578232E-4	0.0	0.016215744240292384	3.563899833031293E-4	0.0
15	8.909749582578232E-4	0.0	0.01728491419020177	3.563899833031293E-4	0.0
16	8.909749582578232E-4	0.0	0.019423254090020547	3.563899833031293E-4	0.0
17	8.909749582578232E-4	0.0	0.01960144908167211	3.563899833031293E-4	0.0
18	8.909749582578232E-4	0.0	0.01995783906497524	3.563899833031293E-4	0.0
19	8.909749582578232E-4	0.0	0.02084881402323306	3.563899833031293E-4	0.0
20	8.909749582578232E-4	0.0	0.021739788981490885	3.563899833031293E-4	0.0
21	8.909749582578232E-4	0.0	0.022096178964794017	5.34584974954694E-4	0.0
22	8.909749582578232E-4	0.0	0.02227437395644558	0.0012473649415609524	0.0
23	8.909749582578232E-4	0.0	0.02263076393974871	0.001425559933212517	0.0
24	8.909749582578232E-4	0.0	0.022808958931400274	0.0016037549248640818	0.0
25	8.909749582578232E-4	0.0	0.022808958931400274	0.0017819499165156465	0.0
26	8.909749582578232E-4	0.0	0.022808958931400274	0.001960144908167211	0.0
27	8.909749582578232E-4	0.0	0.022808958931400274	0.0032075098497281636	0.0
28	8.909749582578232E-4	0.0	0.022808958931400274	0.012117259432306396	0.0
29	8.909749582578232E-4	0.0	0.022808958931400274	0.02726383372268939	0.0
30	8.909749582578232E-4	0.0	0.022808958931400274	0.05185474257060531	0.0
31	8.909749582578232E-4	0.0	0.022808958931400274	0.13239887879711254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	355	0.0	32.30986	1
GTCTAAT	65	2.9831426E-9	28.461536	1
GTATCAA	1240	0.0	25.810482	1
GACGATC	45	0.0038234054	20.555555	15
TTACTCG	55	5.138587E-4	20.181818	19
ACTCGCA	55	5.138587E-4	20.181818	21
ATTAGAC	50	0.007030506	18.499998	3
TACTCGC	50	0.007030506	18.499998	20
CGAACTA	105	4.7864523E-7	17.619047	29
TAACCGT	95	3.5997364E-6	17.526316	7
ACCGTGC	95	3.5997364E-6	17.526316	9
ATCCCGA	75	2.0648923E-4	17.266666	37
ATCAACG	1985	0.0	16.775818	3
ACGGGGT	155	3.9835868E-10	16.709677	16
CATCCCG	90	4.4394365E-5	16.444445	36
TCAACGC	2015	0.0	16.434242	4
CAACGCA	2030	0.0	16.312807	5
AACGCAG	2065	0.0	16.215496	6
TATCAAC	2010	0.0	16.106966	2
AACCGTG	105	9.325986E-6	15.857142	8
>>END_MODULE