FastQCFastQC Report
Fri 10 Feb 2017
ERR1633118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390140
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18310.46931870610550064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.34372276618649716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6750.17301481519454556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.026.2580641
CGAGTCG451.3210245E-424.66666621
GTATCAA9300.024.6666661
ACCGTAT450.003821347420.5555558
TCCGGCG555.1347265E-420.18181816
TCCGCCG555.1347265E-420.18181831
GTCCGGC555.1347265E-420.18181815
CGCCGTC555.1347265E-420.18181826
GGCGAGT555.1347265E-420.18181819
CGTCCGC555.1347265E-420.18181829
GCCGTCC555.1347265E-420.18181827
TGTCCGG555.1347265E-420.18181814
TCGCCGT609.221913E-418.525
TTAGGAG500.007026755318.53
GCACCGC801.6122136E-518.510
AACGGCC801.6122136E-518.537
CATACTA500.007026755318.55
TTAACGG801.6122136E-518.535
TGCTCAC500.007026755318.510
TCATACT609.221913E-418.54
Produced by FastQC (version 0.11.5)