##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633118.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390140
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7331240067668	33.0	33.0	33.0	27.0	33.0
2	31.24061362587789	33.0	33.0	33.0	27.0	33.0
3	31.683859640129185	33.0	33.0	33.0	27.0	33.0
4	31.806167016968267	33.0	33.0	33.0	27.0	33.0
5	31.91115753319321	33.0	33.0	33.0	33.0	33.0
6	34.81416414620393	37.0	37.0	37.0	27.0	37.0
7	34.94797508586661	37.0	37.0	37.0	27.0	37.0
8	35.15664376890347	37.0	37.0	37.0	33.0	37.0
9	35.22366330035372	37.0	37.0	37.0	33.0	37.0
10	35.25113036345927	37.0	37.0	37.0	33.0	37.0
11	35.30568513866817	37.0	37.0	37.0	33.0	37.0
12	35.21637360947352	37.0	37.0	37.0	33.0	37.0
13	35.264038550264004	37.0	37.0	37.0	33.0	37.0
14	35.2119854411237	37.0	37.0	37.0	33.0	37.0
15	35.27075152509356	37.0	37.0	37.0	33.0	37.0
16	35.23901676321321	37.0	37.0	37.0	33.0	37.0
17	35.24136463833496	37.0	37.0	37.0	33.0	37.0
18	35.22205874814169	37.0	37.0	37.0	33.0	37.0
19	35.259494027784896	37.0	37.0	37.0	33.0	37.0
20	35.14667811554826	37.0	37.0	37.0	33.0	37.0
21	35.22900497257395	37.0	37.0	37.0	33.0	37.0
22	35.23009945147896	37.0	37.0	37.0	33.0	37.0
23	35.23194494284103	37.0	37.0	37.0	33.0	37.0
24	35.236402317116934	37.0	37.0	37.0	33.0	37.0
25	35.228569231557906	37.0	37.0	37.0	33.0	37.0
26	35.150981698877324	37.0	37.0	37.0	33.0	37.0
27	35.10415748192956	37.0	37.0	37.0	33.0	37.0
28	35.11122417593684	37.0	37.0	37.0	33.0	37.0
29	35.13265238119649	37.0	37.0	37.0	33.0	37.0
30	35.105751781411804	37.0	37.0	37.0	33.0	37.0
31	35.10057671605065	37.0	37.0	37.0	33.0	37.0
32	35.13854770082534	37.0	37.0	37.0	33.0	37.0
33	35.10094837750551	37.0	37.0	37.0	33.0	37.0
34	35.05202491413339	37.0	37.0	37.0	27.0	37.0
35	35.02643922694418	37.0	37.0	37.0	27.0	37.0
36	35.01567129748295	37.0	37.0	37.0	27.0	37.0
37	35.03364176962116	37.0	37.0	37.0	27.0	37.0
38	34.87896139847234	37.0	37.0	37.0	27.0	37.0
39	34.94666273645358	37.0	37.0	37.0	27.0	37.0
40	34.98754549648844	37.0	37.0	37.0	27.0	37.0
41	34.999659096734504	37.0	37.0	37.0	27.0	37.0
42	34.869754447121544	37.0	37.0	37.0	27.0	37.0
43	34.692602655457016	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	18.0
17	28.0
18	22.0
19	17.0
20	35.0
21	77.0
22	210.0
23	495.0
24	1094.0
25	1908.0
26	3143.0
27	4821.0
28	6687.0
29	9142.0
30	12063.0
31	15412.0
32	19868.0
33	26088.0
34	37121.0
35	66709.0
36	185167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30440354744451	20.141231352847697	13.583585379607321	24.970779720100477
2	15.925565181729636	23.04275388322141	38.02301737837699	23.008663556671962
3	18.1352847695699	26.574819295637464	30.211975188393907	25.07792074639873
4	12.81078587173835	17.88127338903983	38.89834418413903	30.40959655508279
5	14.415338083764803	36.57917670579792	34.746244937714664	14.259240272722613
6	29.72599579638079	39.4930025119188	17.362228943456195	13.418772748244221
7	26.72168964986928	32.2563695083816	23.18731737325063	17.834623468498485
8	24.718818885528272	35.56210591069872	20.70051776285436	19.018557440918645
9	26.320551596862664	15.2158199620649	20.03972932793356	38.42389911313887
10	15.016148049418158	27.88486184446609	33.61178038652791	23.487209719587838
11	34.30896601220075	23.353667914082124	24.19541702978418	18.141949043932946
12	23.109140308607167	25.050494694212333	30.724355359614496	21.116009637566002
13	28.43876557133337	22.055672322755935	26.047060029732915	23.45850207617778
14	22.316091659404318	20.832265340646945	27.83898087865894	29.012662121289797
15	24.076229045983492	28.34085200184549	24.461988004306146	23.120930947864867
16	22.932793356231095	28.245245296560213	25.196339775465216	23.625621571743476
17	22.52832316604296	27.198441585072025	26.50433177833598	23.768903470549034
18	23.49515558517455	26.47049777003127	27.72440662326344	22.309940021530732
19	24.823140411134464	26.192905110985798	27.28661506126006	21.697339416619673
20	24.539908750704875	25.910186087045677	27.321730660788436	22.228174501461016
21	23.468754805967087	26.52765673860665	27.38734813144	22.61624032398626
22	23.153483364945917	26.473317270723328	27.369149536063976	23.004049828266776
23	23.086584303070694	26.28261649664223	27.637002101809603	22.99379709847747
24	23.268826575075614	26.500230686420263	26.98621007843338	23.244732660070746
25	23.373148100681806	26.43281898805557	27.628799917978164	22.56523299328446
26	23.418516429999485	26.62762085405239	27.217152816937514	22.736709899010613
27	23.433126569949252	26.64223099400215	27.19228994719844	22.732352488850157
28	23.08914748551802	26.43666376172656	27.36735530835085	23.10683344440457
29	23.628441072435535	26.44255908135541	26.929051109857998	22.999948736351055
30	23.43722766186497	26.4471728097606	27.381196493566414	22.734403034808018
31	23.53744809555544	26.21341057056441	27.14000102527298	23.109140308607167
32	22.541651714769056	26.303378274465576	27.604706002973288	23.550264007792073
33	22.786691956733478	26.322345824575795	27.96508945506741	22.925872763623314
34	23.019941559440202	26.225970164556312	27.51807043625365	23.236017839749834
35	23.024298969600657	26.79884144153381	27.185881991080123	22.99097759778541
36	23.33111190854565	26.577382478084786	27.2335571846004	22.85794842876916
37	23.471317988414413	26.22391961859845	27.11411288255498	23.190649510432152
38	23.17014405085354	26.674527092838467	26.959296662736453	23.19603219357154
39	22.8033526426411	26.2815912236633	27.57548572307377	23.33957041062183
40	23.034808017634695	26.66581227251756	27.49653970369611	22.802840006151637
41	23.18731737325063	26.060388578459015	27.48321115497001	23.26908289332035
42	22.81258009945148	26.68631773209617	27.46860101502025	23.0325011534321
43	23.177577279950786	26.0029732916389	27.368380581329777	23.451068847080535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	18.5
2	32.0
3	116.5
4	201.0
5	201.0
6	268.5
7	336.0
8	309.0
9	282.0
10	351.0
11	420.0
12	420.0
13	703.5
14	987.0
15	1740.5
16	2494.0
17	2798.0
18	3102.0
19	3102.0
20	2870.0
21	2638.0
22	2911.5
23	3185.0
24	4191.0
25	5197.0
26	5197.0
27	6543.0
28	7889.0
29	9435.5
30	10982.0
31	12942.5
32	14903.0
33	14903.0
34	16996.5
35	19090.0
36	20800.5
37	22511.0
38	24253.5
39	25996.0
40	25996.0
41	27068.0
42	28140.0
43	28757.0
44	29374.0
45	29619.5
46	29865.0
47	29865.0
48	29817.5
49	29770.0
50	29612.5
51	29455.0
52	28387.0
53	27319.0
54	27319.0
55	25658.5
56	23998.0
57	21685.0
58	19372.0
59	17793.5
60	16215.0
61	16215.0
62	14289.0
63	12363.0
64	10277.5
65	8192.0
66	7069.5
67	5947.0
68	5947.0
69	4972.5
70	3998.0
71	3226.5
72	2455.0
73	1993.0
74	1531.0
75	1531.0
76	1173.0
77	815.0
78	638.0
79	461.0
80	367.0
81	273.0
82	273.0
83	206.0
84	139.0
85	129.0
86	119.0
87	88.5
88	58.0
89	58.0
90	40.5
91	23.0
92	15.5
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	390140.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.39768322848535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.28625963441227	64.64118341668478
2	6.544813615023404	9.47658685779885
3	1.6779103904587915	3.644304748026592
4	0.7615430034671905	2.2053579651954793
5	0.3941721105132076	1.4268573797219364
6	0.21865334076510815	0.9497997180937418
7	0.16520362413677006	0.8372251753916146
8	0.1306097646470764	0.756467549397285
9	0.09859297374126375	0.6424112593326948
>10	0.6440967084913768	8.749093261298627
>50	0.04759730819663638	2.414211663302256
>100	0.029481914769792728	3.267209115401821
>500	3.5520379240714127E-4	0.17358253859867998
>1k	7.104075848142825E-4	0.8157093517555746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1831	0.46931870610550064	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1341	0.34372276618649716	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	675	0.17301481519454556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.563182447326601E-4	0.0	0.0
2	0.0	0.0	0.0010252729789306404	0.0	0.0
3	0.0	0.0	0.0015379094683959603	0.0	0.0
4	0.0	0.0	0.0020505459578612807	0.0	0.0
5	0.0	0.0	0.002306864202593941	0.0	0.0
6	0.0	0.0	0.002819500692059261	0.0	0.0
7	0.0	0.0	0.006151637873583841	2.563182447326601E-4	0.0
8	0.0	0.0	0.007945865586712461	2.563182447326601E-4	0.0
9	0.0	0.0	0.009996411544573742	5.126364894653202E-4	0.0
10	7.689547341979802E-4	0.0	0.011278002768237044	5.126364894653202E-4	0.0
11	7.689547341979802E-4	0.0	0.016404367662890246	0.0010252729789306404	0.0
12	7.689547341979802E-4	0.0	0.017685958886553544	0.0010252729789306404	0.0
13	7.689547341979802E-4	0.0	0.018967550110216846	0.0010252729789306404	0.0
14	7.689547341979802E-4	0.0	0.020505459578612807	0.0010252729789306404	0.0
15	7.689547341979802E-4	0.0	0.021787050802276105	0.0010252729789306404	0.0
16	7.689547341979802E-4	0.0	0.022812323781206748	0.0010252729789306404	0.0
17	7.689547341979802E-4	0.0	0.023324960270672067	0.0010252729789306404	0.0
18	7.689547341979802E-4	0.0	0.023837596760137386	0.0012815912236633004	0.0
19	7.689547341979802E-4	0.0	0.024093915004870046	0.0012815912236633004	0.0
20	7.689547341979802E-4	0.0	0.025119187983800688	0.0012815912236633004	0.0
21	7.689547341979802E-4	0.0	0.025888142717998667	0.0017942277131286204	0.0
22	7.689547341979802E-4	0.0	0.026400779207463986	0.002563182447326601	0.0
23	7.689547341979802E-4	0.0	0.026657097452196646	0.002563182447326601	0.0
24	7.689547341979802E-4	0.0	0.026657097452196646	0.002819500692059261	0.0
25	7.689547341979802E-4	0.0	0.026657097452196646	0.002819500692059261	0.0
26	7.689547341979802E-4	0.0	0.026657097452196646	0.0033321371815245807	0.0
27	7.689547341979802E-4	0.0	0.026657097452196646	0.004613728405187882	0.0
28	0.0010252729789306404	0.0	0.026657097452196646	0.014610139949761625	0.0
29	0.0010252729789306404	2.563182447326601E-4	0.026657097452196646	0.03434664479417645	0.0
30	0.0010252729789306404	2.563182447326601E-4	0.026657097452196646	0.055877377351719895	0.0
31	0.0010252729789306404	2.563182447326601E-4	0.026657097452196646	0.12175116624801353	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	310	0.0	26.258064	1
CGAGTCG	45	1.3210245E-4	24.666666	21
GTATCAA	930	0.0	24.666666	1
ACCGTAT	45	0.0038213474	20.555555	8
TCCGGCG	55	5.1347265E-4	20.181818	16
TCCGCCG	55	5.1347265E-4	20.181818	31
GTCCGGC	55	5.1347265E-4	20.181818	15
CGCCGTC	55	5.1347265E-4	20.181818	26
GGCGAGT	55	5.1347265E-4	20.181818	19
CGTCCGC	55	5.1347265E-4	20.181818	29
GCCGTCC	55	5.1347265E-4	20.181818	27
TGTCCGG	55	5.1347265E-4	20.181818	14
TCGCCGT	60	9.221913E-4	18.5	25
TTAGGAG	50	0.0070267553	18.5	3
GCACCGC	80	1.6122136E-5	18.5	10
AACGGCC	80	1.6122136E-5	18.5	37
CATACTA	50	0.0070267553	18.5	5
TTAACGG	80	1.6122136E-5	18.5	35
TGCTCAC	50	0.0070267553	18.5	10
TCATACT	60	9.221913E-4	18.5	4
>>END_MODULE