FastQCFastQC Report
Fri 10 Feb 2017
ERR1633117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385627
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24180.6270307836328888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.4792195567219101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8450.21912366094697724No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8410.2180863891791809No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7720.200193451184694No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7450.1931918667520687No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7270.18852414379698518No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6800.17633620052537816No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6660.17270574933809096No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6030.15636871899529856No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5960.15455349340165497No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5830.15118236015631684No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5820.15092304221436778No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5660.1467739551431824No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5650.1465146372012333No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5510.14288418601394612No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5390.1397723707105571No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5120.1327707862779318No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC5090.1319928324520845No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.1309555606842882No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4910.127325109497001No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4880.12654715567115374No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4880.12654715567115374No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4810.12473193007751014No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4790.12421329419361196No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4670.12110147889022294No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4560.11824898152878299No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4530.11747102770293574No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4510.11695239181903758No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4490.11643375593513938No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA4490.11643375593513938No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA4290.11124739709615769No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4260.11046944327031043No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT4190.10865421767666683No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4190.10865421767666683No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA4130.10709831002497232No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT4090.10606103825717597No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4090.10606103825717597No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG4060.10528308443132874No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3950.10243058706988879No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA3910.10139331530209243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA200.001840419637.016
AGAGACG200.001840419637.08
CGCATCT401.5964179E-632.37515
GCCGGCA658.731149E-1131.30769215
GCTGAGC303.5950833E-430.8333329
CCGCTCT601.2660166E-930.83333228
ATGCCCA303.5950833E-430.83333232
GCCCCCT250.0054918729.630
GTATTGA250.0054918729.61
AGCTTCG702.055458E-1029.07142821
CGCTCTC652.9776857E-928.46153829
CCGGAGC405.930052E-527.75000235
CGGCAGC754.6020432E-1027.13333317
GCCGCTC706.5629138E-926.4285727
GCTCTCT706.5629138E-926.4285730
CCGGCAG809.731593E-1025.43750216
CGCCGGC809.731593E-1025.43750214
AGGCCGG451.3209853E-424.66666832
TTCGCCG851.9663275E-923.94117724
GCTCGCA551.8984116E-523.54545412
Produced by FastQC (version 0.11.5)