##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633117.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	385627
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51008357817269	33.0	33.0	33.0	27.0	33.0
2	31.04432262263794	33.0	33.0	33.0	27.0	33.0
3	31.48252586048176	33.0	33.0	33.0	27.0	33.0
4	31.6370508289098	33.0	33.0	33.0	27.0	33.0
5	31.758217137285513	33.0	33.0	33.0	27.0	33.0
6	34.61112162789431	37.0	37.0	37.0	27.0	37.0
7	34.82080870893376	37.0	37.0	37.0	27.0	37.0
8	34.97503286854914	37.0	37.0	37.0	27.0	37.0
9	35.06992248986715	37.0	37.0	37.0	33.0	37.0
10	35.080440425592606	37.0	37.0	37.0	33.0	37.0
11	35.11958965528866	37.0	37.0	37.0	33.0	37.0
12	35.05397962279612	37.0	37.0	37.0	27.0	37.0
13	35.080458577848546	37.0	37.0	37.0	33.0	37.0
14	35.04894885472231	37.0	37.0	37.0	27.0	37.0
15	35.05230702207055	37.0	37.0	37.0	27.0	37.0
16	35.062168883402876	37.0	37.0	37.0	27.0	37.0
17	35.03647825489398	37.0	37.0	37.0	27.0	37.0
18	35.04995760151649	37.0	37.0	37.0	27.0	37.0
19	35.05225775166158	37.0	37.0	37.0	27.0	37.0
20	35.03871876191242	37.0	37.0	37.0	27.0	37.0
21	35.05139162973547	37.0	37.0	37.0	27.0	37.0
22	35.04497869703107	37.0	37.0	37.0	27.0	37.0
23	35.03693465447181	37.0	37.0	37.0	27.0	37.0
24	35.01795776747998	37.0	37.0	37.0	27.0	37.0
25	35.01776587220293	37.0	37.0	37.0	27.0	37.0
26	34.96271785948598	37.0	37.0	37.0	27.0	37.0
27	34.91178262932834	37.0	37.0	37.0	27.0	37.0
28	34.884222318457994	37.0	37.0	37.0	27.0	37.0
29	34.90917129765291	37.0	37.0	37.0	27.0	37.0
30	34.850140161347625	37.0	37.0	37.0	27.0	37.0
31	34.82750170501547	37.0	37.0	37.0	27.0	37.0
32	34.852235450318574	37.0	37.0	37.0	27.0	37.0
33	34.83393019679639	37.0	37.0	37.0	27.0	37.0
34	34.75613481421166	37.0	37.0	37.0	27.0	37.0
35	34.743244119317374	37.0	37.0	37.0	27.0	37.0
36	34.71183812336792	37.0	37.0	37.0	27.0	37.0
37	34.727747279106495	37.0	37.0	37.0	27.0	37.0
38	34.58068807422717	37.0	37.0	37.0	27.0	37.0
39	34.6472290581313	37.0	37.0	37.0	27.0	37.0
40	34.65404652682514	37.0	37.0	37.0	27.0	37.0
41	34.65887762008366	37.0	37.0	37.0	27.0	37.0
42	34.53751682325149	37.0	37.0	37.0	27.0	37.0
43	34.31020649487717	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	16.0
17	20.0
18	24.0
19	11.0
20	44.0
21	114.0
22	327.0
23	677.0
24	1346.0
25	2488.0
26	3923.0
27	5681.0
28	7910.0
29	10478.0
30	13623.0
31	16928.0
32	21342.0
33	27637.0
34	38041.0
35	66950.0
36	168037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.607444499477474	22.35579977543066	13.684726432537142	24.35202929255472
2	15.982542716147988	23.383736097316838	38.112476564141	22.52124462239418
3	16.52451721482158	28.996672950804793	30.664087317537415	23.814722516836216
4	11.441626234677551	18.947584064393833	41.88762716303579	27.723162537892836
5	12.17653328216126	38.92388240449968	36.56357049687911	12.336013816459946
6	26.573346783290592	43.08437946513081	17.637250503725102	12.705023247853495
7	25.21218690599984	33.27438172119691	24.90359855508043	16.609832817722825
8	23.400332445601578	36.40045951139314	20.706019028750582	19.493189014254707
9	25.66806784794633	14.86695692988302	21.344978437713127	38.11999678445752
10	15.188251859957939	27.662222821534797	34.792169635424905	22.357355683082357
11	32.63568163017631	24.98839552209778	24.80920682421096	17.56671602351495
12	22.2598521369095	27.76076363947545	29.615664878237258	20.36371934537779
13	26.9537662041299	23.304644125022367	26.307545892792778	23.43404377805496
14	20.97908082162297	22.097259787307426	29.124257378243744	27.799402012825862
15	23.902890617098908	29.204645940247957	25.870076524724666	21.022386917928465
16	21.46089355776437	29.20257139671237	26.285503867727105	23.051031177796162
17	21.12896659206954	28.16063190596094	27.053603611780296	23.656797890189225
18	21.501347156708427	28.080243343956724	29.088990138138666	21.32941936119618
19	24.073262504959455	26.77198432682359	29.064095615711555	20.0906575525054
20	22.92137220682162	26.872859006241782	29.259102708057267	20.946666078879332
21	21.506274197605457	28.316222671130394	28.992264545791656	21.18523858547249
22	21.411104512910143	28.009708863746575	28.81904016056967	21.760146462773612
23	21.46089355776437	27.862416272719493	29.72457841385588	20.952111755660262
24	21.564620734544	28.808667442891707	27.112987420486633	22.513724402077656
25	21.75158897068929	27.977812756886838	29.396022581406385	20.874575691017487
26	21.359240924520325	28.629738062946835	29.131777598560266	20.87924341397257
27	21.57032572926688	28.334893562950725	28.042382924432157	22.052397783350234
28	20.807412344052672	27.764394090662737	30.183311853163808	21.24488171212078
29	23.370251564335483	27.649775560321245	27.86345354448729	21.116519330855983
30	22.67424220814414	26.963360967982013	29.087434230486974	21.274962593386874
31	21.85531614746893	27.6907477951492	29.01534384262513	21.438592214756746
32	20.26388193772739	27.33963130175014	29.532164500929653	22.86432225959282
33	20.343492545905757	27.659111006231413	30.101108065565946	21.896288382296884
34	21.78919007227191	27.142290347926885	29.557836977182617	21.51068260261859
35	21.631524763566865	28.513044989069748	28.584098105163797	21.271332142199586
36	23.09096614085632	28.029676345276656	27.459436190930614	21.419921322936414
37	21.67560881369821	27.317329958742516	28.291846784587175	22.715214442972094
38	21.84624001950071	27.899758056360163	28.17748757218763	22.0765143519515
39	20.92721723323315	27.81029336638773	28.999525448166235	22.26296395221289
40	20.954186299195854	28.774437474554425	29.517124060296606	20.754252165953112
41	21.196129939034353	27.170296685657387	28.87479351808872	22.75877985721954
42	20.675419511600587	28.910320076135747	28.77677133603197	21.637489076231695
43	21.92610994562103	26.730493456111738	28.92925028589803	22.41414631236921
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	30.0
2	39.0
3	143.0
4	247.0
5	247.0
6	326.0
7	405.0
8	397.0
9	389.0
10	527.0
11	665.0
12	665.0
13	1081.0
14	1497.0
15	2419.5
16	3342.0
17	3670.5
18	3999.0
19	3999.0
20	3369.0
21	2739.0
22	2783.0
23	2827.0
24	3668.5
25	4510.0
26	4510.0
27	5899.0
28	7288.0
29	9159.5
30	11031.0
31	14162.0
32	17293.0
33	17293.0
34	20746.5
35	24200.0
36	26597.0
37	28994.0
38	30820.0
39	32646.0
40	32646.0
41	34348.5
42	36051.0
43	36215.0
44	36379.0
45	34860.5
46	33342.0
47	33342.0
48	31811.5
49	30281.0
50	29042.0
51	27803.0
52	25586.5
53	23370.0
54	23370.0
55	22106.5
56	20843.0
57	16258.0
58	11673.0
59	10587.0
60	9501.0
61	9501.0
62	7989.5
63	6478.0
64	4750.0
65	3022.0
66	2525.5
67	2029.0
68	2029.0
69	1606.0
70	1183.0
71	929.0
72	675.0
73	561.5
74	448.0
75	448.0
76	303.0
77	158.0
78	132.5
79	107.0
80	87.5
81	68.0
82	68.0
83	51.0
84	34.0
85	31.0
86	28.0
87	23.0
88	18.0
89	18.0
90	10.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	385627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.86492143542727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.39347080255875	33.18810533839898
2	5.743953899825447	4.464766340908642
3	2.012027310535578	2.3459185004969783
4	1.133287612200149	1.7618053604760702
5	0.7717693698473524	1.4997377962693281
6	0.6218216674911282	1.4500230152333444
7	0.474143307378401	1.2899279673276638
8	0.3766467273792731	1.1710676374804994
9	0.32398736123568767	1.1332569006446758
>10	2.532953689817602	20.108605657848834
>50	0.36317741408737503	9.917112146827856
>100	0.23945763566200554	17.62315447999066
>500	0.011972881783100277	2.9436983477380947
>1k	0.0013303201981222528	1.102820510358366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2418	0.6270307836328888	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1848	0.4792195567219101	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.21912366094697724	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	841	0.2180863891791809	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	772	0.200193451184694	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	745	0.1931918667520687	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	727	0.18852414379698518	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	680	0.17633620052537816	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	666	0.17270574933809096	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	603	0.15636871899529856	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	596	0.15455349340165497	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	583	0.15118236015631684	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	582	0.15092304221436778	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	566	0.1467739551431824	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	565	0.1465146372012333	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	551	0.14288418601394612	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	539	0.1397723707105571	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	512	0.1327707862779318	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	509	0.1319928324520845	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.1309555606842882	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	491	0.127325109497001	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	488	0.12654715567115374	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	488	0.12654715567115374	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	481	0.12473193007751014	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	479	0.12421329419361196	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	467	0.12110147889022294	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	456	0.11824898152878299	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	453	0.11747102770293574	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	451	0.11695239181903758	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	449	0.11643375593513938	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	449	0.11643375593513938	No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA	429	0.11124739709615769	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	426	0.11046944327031043	No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT	419	0.10865421767666683	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	419	0.10865421767666683	No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA	413	0.10709831002497232	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT	409	0.10606103825717597	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	409	0.10606103825717597	No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG	406	0.10528308443132874	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	395	0.10243058706988879	No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA	391	0.10139331530209243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0010372717677963421	0.0	0.0
2	0.0	0.0	0.001555907651694513	0.0	0.0
3	0.0	0.0	0.0018152255936435986	2.5931794194908553E-4	0.0
4	0.0	0.0	0.0018152255936435986	5.186358838981711E-4	0.0
5	0.0	0.0	0.0023338614775417696	5.186358838981711E-4	0.0
6	0.0	0.0	0.004408405013134453	5.186358838981711E-4	0.0
7	0.0	0.0	0.007779538258472565	5.186358838981711E-4	0.0
8	2.5931794194908553E-4	0.0	0.009594763852116163	5.186358838981711E-4	0.0
9	5.186358838981711E-4	0.0	0.013484532981352446	0.001555907651694513	0.0
10	0.001555907651694513	0.0	0.016337030342792387	0.001555907651694513	0.0
11	0.001555907651694513	0.0	0.028265655672450322	0.001555907651694513	0.0
12	0.001555907651694513	0.0	0.0321554248016866	0.001555907651694513	0.0
13	0.001555907651694513	0.0	0.0376011015826174	0.0018152255936435986	0.0
14	0.0018152255936435986	0.0	0.038897691292362827	0.0018152255936435986	0.0
15	0.0018152255936435986	0.0	0.04200950659575185	0.0018152255936435986	0.0
16	0.0018152255936435986	0.0	0.04538063984108996	0.0018152255936435986	0.0
17	0.0018152255936435986	0.0	0.045899275724988135	0.0018152255936435986	0.0
18	0.0018152255936435986	0.0	0.04745518337668265	0.0020745435355926842	0.0
19	0.0018152255936435986	0.0	0.04849245514447899	0.0020745435355926842	0.0
20	0.0018152255936435986	0.0	0.049529726912275335	0.002593179419490855	0.0
21	0.0018152255936435986	0.0	0.050566998680071674	0.002593179419490855	0.0
22	0.0018152255936435986	0.0	0.05134495250591893	0.0028524973614399408	0.0
23	0.0018152255936435986	0.0	0.05212290633176619	0.0036304511872871973	0.0
24	0.0018152255936435986	0.0	0.05238222427371527	0.0038897691292362827	0.0
25	0.0018152255936435986	0.0	0.05238222427371527	0.0041490870711853685	0.0
26	0.0018152255936435986	0.0	0.05238222427371527	0.004408405013134453	0.0
27	0.0018152255936435986	0.0	0.05238222427371527	0.0059643126648289664	0.0
28	0.0018152255936435986	0.0	0.05238222427371527	0.02074543535592684	0.0
29	0.0018152255936435986	0.0	0.05238222427371527	0.04641791160888631	0.0
30	0.0018152255936435986	0.0	0.05238222427371527	0.08687151055294365	0.0
31	0.0018152255936435986	0.0	0.05238222427371527	0.200193451184694	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCA	20	0.0018404196	37.0	16
AGAGACG	20	0.0018404196	37.0	8
CGCATCT	40	1.5964179E-6	32.375	15
GCCGGCA	65	8.731149E-11	31.307692	15
GCTGAGC	30	3.5950833E-4	30.833332	9
CCGCTCT	60	1.2660166E-9	30.833332	28
ATGCCCA	30	3.5950833E-4	30.833332	32
GCCCCCT	25	0.00549187	29.6	30
GTATTGA	25	0.00549187	29.6	1
AGCTTCG	70	2.055458E-10	29.071428	21
CGCTCTC	65	2.9776857E-9	28.461538	29
CCGGAGC	40	5.930052E-5	27.750002	35
CGGCAGC	75	4.6020432E-10	27.133333	17
GCCGCTC	70	6.5629138E-9	26.42857	27
GCTCTCT	70	6.5629138E-9	26.42857	30
CCGGCAG	80	9.731593E-10	25.437502	16
CGCCGGC	80	9.731593E-10	25.437502	14
AGGCCGG	45	1.3209853E-4	24.666668	32
TTCGCCG	85	1.9663275E-9	23.941177	24
GCTCGCA	55	1.8984116E-5	23.545454	12
>>END_MODULE