FastQCFastQC Report
Fri 10 Feb 2017
ERR1633116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219410
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5480.24976072193610135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.2009935736748553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2330.106193883596919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA900.030.8333321
CGTGCAA358.846447E-426.4285711
AGTAGTG358.846447E-426.428575
GTATCAA3300.023.5454561
ACGGGAT400.001926237523.12500216
TAACCGT400.001926237523.1250027
ATACAAT400.001926237523.1250026
CCAACGG603.708627E-521.58333231
TAGTACC450.003816096620.5555554
CCGTGCA555.1248877E-420.18181810
TATACTG759.209862E-619.7333345
GTACCCT500.007017194818.51
CAACGGA701.2135015E-418.532
TAGAATA801.6069767E-518.52
CCTCGAT500.007017194818.535
CTAACCG500.007017194818.56
ATAGTAC500.007017194818.53
GCTCGGA500.007017194818.511
ATCAACG4250.017.8470573
TCAACGC4250.017.8470574
Produced by FastQC (version 0.11.5)