##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219410
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.672622031812587	33.0	33.0	33.0	27.0	33.0
2	30.458351943849415	33.0	33.0	33.0	27.0	33.0
3	30.92279750239278	33.0	33.0	33.0	27.0	33.0
4	31.113814320222414	33.0	33.0	33.0	27.0	33.0
5	31.248183765553073	33.0	33.0	33.0	27.0	33.0
6	33.63305683423727	37.0	33.0	37.0	27.0	37.0
7	33.874900870516385	37.0	33.0	37.0	27.0	37.0
8	34.0334305637847	37.0	33.0	37.0	27.0	37.0
9	34.09153183537669	37.0	33.0	37.0	27.0	37.0
10	34.115445968734335	37.0	33.0	37.0	27.0	37.0
11	34.128212023153004	37.0	33.0	37.0	27.0	37.0
12	34.14853926439087	37.0	33.0	37.0	27.0	37.0
13	34.1549245704389	37.0	33.0	37.0	27.0	37.0
14	34.16370721480334	37.0	33.0	37.0	27.0	37.0
15	34.198227063488446	37.0	33.0	37.0	27.0	37.0
16	34.19541041885056	37.0	37.0	37.0	27.0	37.0
17	34.170639442140285	37.0	33.0	37.0	27.0	37.0
18	34.1855931817146	37.0	33.0	37.0	27.0	37.0
19	34.14144751834465	37.0	33.0	37.0	27.0	37.0
20	34.16474636525227	37.0	33.0	37.0	27.0	37.0
21	34.16330158151406	37.0	33.0	37.0	27.0	37.0
22	34.189658629962175	37.0	37.0	37.0	27.0	37.0
23	34.16297798641812	37.0	37.0	37.0	27.0	37.0
24	34.13462923294289	37.0	33.0	37.0	27.0	37.0
25	34.109607583975205	37.0	33.0	37.0	27.0	37.0
26	34.05704844811084	37.0	33.0	37.0	27.0	37.0
27	33.99186454582745	37.0	33.0	37.0	27.0	37.0
28	33.95885784604166	37.0	33.0	37.0	27.0	37.0
29	34.012447017000135	37.0	33.0	37.0	27.0	37.0
30	33.96171550977622	37.0	33.0	37.0	27.0	37.0
31	33.976359327286815	37.0	33.0	37.0	27.0	37.0
32	33.978474089603935	37.0	33.0	37.0	27.0	37.0
33	33.94930951187275	37.0	33.0	37.0	27.0	37.0
34	33.85692994849825	37.0	33.0	37.0	27.0	37.0
35	33.862590583838475	37.0	33.0	37.0	27.0	37.0
36	33.82042751014083	37.0	33.0	37.0	27.0	37.0
37	33.85599562462969	37.0	33.0	37.0	27.0	37.0
38	33.7265302401896	37.0	33.0	37.0	27.0	37.0
39	33.807852878173286	37.0	33.0	37.0	27.0	37.0
40	33.8345107333303	37.0	33.0	37.0	27.0	37.0
41	33.86951369582061	37.0	33.0	37.0	27.0	37.0
42	33.74566792762408	37.0	33.0	37.0	27.0	37.0
43	33.467494644729044	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	15.0
16	15.0
17	21.0
18	18.0
19	24.0
20	72.0
21	215.0
22	503.0
23	970.0
24	1707.0
25	2770.0
26	3971.0
27	5488.0
28	6972.0
29	8732.0
30	10790.0
31	12722.0
32	15455.0
33	19044.0
34	25851.0
35	41053.0
36	63000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.11485347067135	21.80073834374003	13.246889385169316	24.837518800419307
2	16.056697506950456	24.16981906020692	36.6241283441958	23.149355088646825
3	18.032450663142065	27.583519438494143	30.232441547787246	24.151588350576546
4	13.549974932774258	19.22200446652386	38.3232304817465	28.904790118955383
5	14.693951962080124	37.13777858803154	35.194384941433846	12.973884508454493
6	29.772116129620347	40.084772799781234	17.366118226151954	12.77699284444647
7	26.263616061255185	32.524041748325054	23.98249851875484	17.22984367166492
8	24.203545873023106	36.82329884690761	20.650836333804293	18.322318946264986
9	25.58634519848685	15.73082357230755	20.42751014083223	38.255321088373364
10	14.485666104553118	28.565698919830457	34.68483660726494	22.263798368351488
11	33.73911854518937	23.73364933230026	24.682102000820382	17.845130121689987
12	22.56004739984504	25.77776764960576	30.939793081445693	20.722391869103504
13	27.406225787338773	22.595141515883505	26.57718426689759	23.421448429880133
14	21.52272002187685	21.10295793263753	28.149582972517205	29.224739072968415
15	23.36493323002598	29.337313704935962	24.554031265667017	22.74372179937104
16	22.977074882639805	28.62586026161068	25.629643133858988	22.767421721890525
17	22.05232213663917	28.13226379836835	26.996490588396156	22.818923476596325
18	22.582835786883003	28.002369992251946	27.787703386354313	21.627090834510735
19	23.802926028895673	27.280889658629963	27.605852057791346	21.310332254683015
20	23.501207784513014	27.297297297297295	27.63912310286678	21.562371815322912
21	22.763775579964452	27.50740622578734	27.65598650927487	22.072831684973337
22	22.63069139966273	27.283168497333758	27.63638849642222	22.449751606581287
23	22.570985825623264	27.13139783966091	27.835103231393283	22.462513103322546
24	22.7295929994075	27.813682147577595	26.777722072831683	22.679002780183218
25	22.88774440545098	27.09129027847409	27.63274235449615	22.38822296157878
26	22.930130805341598	27.503304316120502	27.337860626224874	22.228704252313022
27	22.594229980401987	27.440864135636478	27.042523130212842	22.92238275374869
28	22.199079349163668	27.56665603208605	27.767193838020145	22.46707078073014
29	23.183081901463016	27.236680187776308	27.193382252404174	22.386855658356502
30	22.972972972972975	27.253087826443647	27.455448703340778	22.318490497242603
31	22.924661592452487	27.244428239369217	27.581696367531105	22.24921380064719
32	22.518116767695183	27.057563465657903	27.51150813545417	22.912811631192746
33	22.18130440727405	27.225285994257327	28.006927669659543	22.58648192880908
34	22.893213618340095	26.901690898318215	27.725718973611045	22.479376509730642
35	22.609726083587802	27.349710587484616	27.532929219269857	22.50763410965772
36	23.161205049906567	27.288181942482108	27.192926484663417	22.357686522947905
37	22.80889658629962	26.752199079349165	27.501025477416707	22.937878856934507
38	22.69130850918372	27.386627774486122	27.294562690852743	22.627501025477418
39	22.313021284353493	27.359281710040563	27.702930586573082	22.62476641903286
40	22.444282393692173	27.39528736156055	27.865639670024155	22.294790574723123
41	22.529966728954925	26.791850872795226	27.746228521945216	22.931953876304632
42	22.323503942390957	27.55981951597466	27.534752290232902	22.581924251401485
43	22.864044482931497	26.547559363748235	27.731188186500162	22.85720796682011
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	10.0
2	15.0
3	38.0
4	61.0
5	61.0
6	86.0
7	111.0
8	108.5
9	106.0
10	138.0
11	170.0
12	170.0
13	270.5
14	371.0
15	632.5
16	894.0
17	1031.5
18	1169.0
19	1169.0
20	1274.5
21	1380.0
22	1676.5
23	1973.0
24	2573.5
25	3174.0
26	3174.0
27	4078.5
28	4983.0
29	6002.5
30	7022.0
31	8194.0
32	9366.0
33	9366.0
34	11047.5
35	12729.0
36	13744.5
37	14760.0
38	15772.5
39	16785.0
40	16785.0
41	17376.5
42	17968.0
43	18115.0
44	18262.0
45	17856.5
46	17451.0
47	17451.0
48	16771.0
49	16091.0
50	15748.0
51	15405.0
52	14424.5
53	13444.0
54	13444.0
55	12799.5
56	12155.0
57	10767.5
58	9380.0
59	8539.0
60	7698.0
61	7698.0
62	6793.5
63	5889.0
64	4852.5
65	3816.0
66	3171.5
67	2527.0
68	2527.0
69	2109.5
70	1692.0
71	1396.0
72	1100.0
73	882.0
74	664.0
75	664.0
76	500.5
77	337.0
78	263.0
79	189.0
80	154.0
81	119.0
82	119.0
83	88.5
84	58.0
85	54.0
86	50.0
87	40.0
88	30.0
89	30.0
90	19.0
91	8.0
92	5.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	219410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.78778006685644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.91023740081971	70.7170555276067
2	5.424732252857353	8.439557588137008
3	1.4803003122965106	3.4544782537745964
4	0.6738412258358268	2.09666452301193
5	0.3767153216450101	1.4651924293968566
6	0.23852941638186695	1.1132804268592975
7	0.1652158685873627	0.89962429544599
8	0.11268002494756184	0.701210319883907
9	0.08209577301484121	0.5747443142127333
>10	0.4887706897346641	7.059577650951241
>50	0.03281719971552811	1.7021553112917698
>100	0.013478492740306186	1.5266518847124095
>500	5.860214234915733E-4	0.249807474715557
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.24976072193610135	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	441	0.2009935736748553	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	233	0.106193883596919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.557677407593091E-4	0.0
4	0.0	0.0	0.0	4.557677407593091E-4	0.0
5	0.0	0.0	0.0	4.557677407593091E-4	0.0
6	0.0	0.0	4.557677407593091E-4	4.557677407593091E-4	0.0
7	0.0	0.0	0.0022788387037965454	4.557677407593091E-4	0.0
8	0.0	0.0	0.0036461419260744726	4.557677407593091E-4	0.0
9	0.0	0.0	0.0050134451483524	9.115354815186182E-4	0.0
10	0.0	0.0	0.006836516111389636	9.115354815186182E-4	0.0
11	0.0	0.0	0.008659587074426872	9.115354815186182E-4	0.0
12	0.0	0.0	0.009115354815186182	9.115354815186182E-4	0.0
13	0.0	0.0	0.00957112255594549	9.115354815186182E-4	0.0
14	0.0	0.0	0.010482658037464109	9.115354815186182E-4	0.0
15	0.0	0.0	0.012761496741260654	9.115354815186182E-4	0.0
16	0.0	0.0	0.013217264482019963	9.115354815186182E-4	0.0
17	0.0	0.0	0.013217264482019963	9.115354815186182E-4	0.0
18	0.0	0.0	0.013217264482019963	9.115354815186182E-4	0.0
19	0.0	0.0	0.013217264482019963	9.115354815186182E-4	0.0
20	0.0	0.0	0.013673032222779272	0.0013673032222779272	0.0
21	0.0	0.0	0.013673032222779272	0.0027346064445558545	0.0
22	0.0	0.0	0.014128799963538581	0.0027346064445558545	0.0
23	0.0	0.0	0.014128799963538581	0.0036461419260744726	0.0
24	0.0	0.0	0.014128799963538581	0.004101909666833782	0.0
25	0.0	0.0	0.014128799963538581	0.004101909666833782	0.0
26	0.0	0.0	0.014128799963538581	0.004557677407593091	0.0
27	0.0	0.0	0.014128799963538581	0.0050134451483524	0.0
28	0.0	0.0	0.014128799963538581	0.007292283852148945	0.0
29	0.0	0.0	0.014128799963538581	0.016407638667335127	0.0
30	0.0	0.0	0.014128799963538581	0.03372681281618887	0.0
31	0.0	0.0	0.014128799963538581	0.07884781915136047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	90	0.0	30.833332	1
CGTGCAA	35	8.846447E-4	26.42857	11
AGTAGTG	35	8.846447E-4	26.42857	5
GTATCAA	330	0.0	23.545456	1
ACGGGAT	40	0.0019262375	23.125002	16
TAACCGT	40	0.0019262375	23.125002	7
ATACAAT	40	0.0019262375	23.125002	6
CCAACGG	60	3.708627E-5	21.583332	31
TAGTACC	45	0.0038160966	20.555555	4
CCGTGCA	55	5.1248877E-4	20.181818	10
TATACTG	75	9.209862E-6	19.733334	5
GTACCCT	50	0.0070171948	18.5	1
CAACGGA	70	1.2135015E-4	18.5	32
TAGAATA	80	1.6069767E-5	18.5	2
CCTCGAT	50	0.0070171948	18.5	35
CTAACCG	50	0.0070171948	18.5	6
ATAGTAC	50	0.0070171948	18.5	3
GCTCGGA	50	0.0070171948	18.5	11
ATCAACG	425	0.0	17.847057	3
TCAACGC	425	0.0	17.847057	4
>>END_MODULE